Validated using a knockout cell line

Anti-beta Actin antibody [AC-15] (ab6276)

Reviews (94)Q&A (47)References (1949)
Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [AC-15] (ab6276)
  • Western blot - Anti-beta Actin antibody [AC-15] (ab6276)
  • Western blot - Anti-beta Actin antibody [AC-15] (ab6276)
  • Western blot - Anti-beta Actin antibody [AC-15] (ab6276)
  • Western blot - Anti-beta Actin antibody [AC-15] (ab6276)
  • Western blot - Anti-beta Actin antibody [AC-15] (ab6276)

Key features and details

  • Mouse monoclonal [AC-15] to beta Actin
  • Suitable for: ICC/IF, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Cow, Dog, Human, African green monkey, Chinese hamster
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

FITC HRP

Overview

  • Product name

    Anti-beta Actin antibody [AC-15]
    See all beta Actin primary antibodies

  • Description

    Mouse monoclonal [AC-15] to beta Actin

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Dog, Human, African green monkey, Chinese hamster
    Does not react with: Drosophila melanogaster, Dictyostelium discoideum

  • Immunogen

    Synthetic peptide corresponding to beta Actin aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin. Slightly modified ß-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Al?a-Leu-Val-Ile-Asp-Asn-Gl y?-Ser-Gly-Lys, conjugated to KLH.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Epitope

    N-terminal of the beta isoform of actin.

  • Positive control

    • ICC/IF: SV40LT-SMC cells WB: HAP1, HeLa, Jurkat, A431, HEK-293, COS-7, NIH/3T3, PC-12, Rat2, CHO, MDBK and MDCK cell lysates.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (9)
Use a concentration of 5 µg/ml.
WB (76)
1/5000 - 1/16000. Predicted molecular weight: 42 kDa.
Notes
ICC/IF
Use a concentration of 5 µg/ml.
WB
1/5000 - 1/16000. Predicted molecular weight: 42 kDa.

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

  • Involvement in disease

    Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.

  • Sequence similarities

    Belongs to the actin family.

  • Post-translational
    modifications

    ISGylated.

  • Cellular localization

    Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • A26C1A antibody
    • A26C1B antibody
    • ACTB antibody
    • ACTB_HUMAN antibody
    • Actin beta antibody
    • Actin cytoplasmic 1 antibody
    • Actin, cytoplasmic 1, N-terminally processed antibody
    • Actx antibody
    • b actin antibody
    • b-actin antibody
    • Beta cytoskeletal actin antibody
    • Beta-actin antibody
    • BRWS1 antibody
    • E430023M04Rik antibody
    • Melanoma X actin antibody
    • MGC128179 antibody
    • PS1TP5 binding protein 1 antibody
    • PS1TP5BP1 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [AC-15] (ab6276)
    Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [AC-15] (ab6276)

    ab6276 staining beta Actin in SV40LT-SMC cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab6276 at a working concentration of 5μg/ml and ab190573, Rabbit monoclonal [EP1332Y] to alpha Tubulin (Alexa Fluor® 647, shown in red) at 1/250 overnight at +4°C, followed by a further incubation at room temperature for 1h with an anti-mouse AlexaFluor® 488 (ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.
    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Western blot - Anti-beta Actin antibody [AC-15] (ab6276)
    Western blot - Anti-beta Actin antibody [AC-15] (ab6276)
    All lanes : Anti-beta Actin antibody [AC-15] (ab6276) at 1/5000 dilution

    Lane 1 : Wild-type HAP1 cell lysate (20 µg)
    Lane 2 : Beta actin knockout HAP1 cell lysate (20 µg)

    Secondary
    All lanes : Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) at 1/10000 dilution

    Predicted band size: 42 kDa



    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Beta actin knockout HAP1 cell lysate (20 µg)

    Lanes 1 and 2: Merged signal (red and green). Green - beta actin, ab6276 observed at 42 kDa. Red - loading control, ab181602 observed at 37 kDa.

    Ab6276 was shown to specifically react with beta actin in wild-type HAP1 cells. No band was observed when beta actin knockout samples were used. Wild-type and beta actin knockout samples were subjected to SDS-PAGE. ab6276 (beta actin) and ab181602 (loading control to GAPDH) were diluted 1/5000 and 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-beta Actin antibody [AC-15] (ab6276)
    Western blot - Anti-beta Actin antibody [AC-15] (ab6276)This image is courtesy of an Abreview submitted by Dr Mark Elliott

    Western Blot of ab6276 (used as loading control) with whole tissue lysate of human grey matter from BA20 (temporal cortex).  Ab6276 was diluted 1/50000 and incubated with the sample for 16 hours at 4°C.  5 µg of lysate was loaded onto the gel, which was blocked with 5% milk for 1 hour at 20°C.  An Alexa Fluor® 680 conjugated goat anti-mouse antibody, diluted 1/5000, was used as the secondary.

    Bands below actin in image are synaptophysin (SYN).

    See Abreview

  • Western blot - Anti-beta Actin antibody [AC-15] (ab6276)
    Western blot - Anti-beta Actin antibody [AC-15] (ab6276)
    All lanes : Anti-beta Actin antibody [AC-15] (ab6276) at 1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 3 : COS-7 (african green monkey kidney fibroblast-like cell line) cell lysate
    Lane 4 : NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate
    Lane 5 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate
    Lane 6 : Rat2 (rat fibroblast cell line) cell lysate
    Lane 7 : CHO (chinese hamster ovary cell line) cell lysate
    Lane 8 : MDBK (bovine kidney cell line) cell lysate
    Lane 9 : MDCK (canine kidney cell line) cell lysate

    Secondary
    All lanes : Goat Anti-Mouse IgG-Peroxidase

    Developed using the ECL technique.

    Predicted band size: 42 kDa

  • Western blot - Anti-beta Actin antibody [AC-15] (ab6276)
    Western blot - Anti-beta Actin antibody [AC-15] (ab6276)
    All lanes : Anti-beta Actin antibody [AC-15] (ab6276) at 1/5000 dilution

    Lane 1 : HeLa nuclear
    Lane 2 : HeLa whole cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : Jurkat cell lysate
    Lane 5 : HEK293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Alexa Fluor anti mouse at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

  • Western blot - Anti-beta Actin antibody [AC-15] (ab6276)
    Western blot - Anti-beta Actin antibody [AC-15] (ab6276)This image is courtesy of David Grimm, Yale University, USA.

    MDCK cells induced with increasing amounts of doxycycline to control expression of the gene of interest. All cells were normalized for loading with an albumin protein standard assay. Anti-beta actin (ab6276) was used at a concentration of 1:5000 in a milk blocking solution. B-actin blotting confirms the albumin assay in showing that an equal amount of lysate was loaded in each lane.

References (1949)

Publishing research using ab6276? Please let us know so that we can cite the reference in this datasheet.

ab6276 has been referenced in 1949 publications.

  • Itai T  et al. De novo variants in CELF2 that disrupt the nuclear localization signal cause developmental and epileptic encephalopathy. Hum Mutat 42:66-76 (2021). PubMed: 33131106
  • Cooley MM  et al. Deficient Endoplasmic Reticulum Acetyl-CoA Import in Pancreatic Acinar Cells Leads to Chronic Pancreatitis. Cell Mol Gastroenterol Hepatol 11:725-738 (2021). PubMed: 33080365
  • Fløyel T  et al. SKAP2, a Candidate Gene for Type 1 Diabetes, Regulates ß-Cell Apoptosis and Glycemic Control in Newly Diagnosed Patients. Diabetes 70:464-476 (2021). PubMed: 33203694
  • Choi YS  et al. Chloroform extract of Citrus unshiu Markovich peel induces apoptosis and inhibits stemness in HeLa human cervical cancer cells. Mol Med Rep 23:N/A (2021). PubMed: 33236129
  • Fujihara K  et al. Genetic deletion of the 67-kDa isoform of glutamate decarboxylase alters conditioned fear behavior in rats. FEBS Open Bio 11:340-353 (2021). PubMed: 33325157
View all Publications for this product

Customer reviews and Q&As

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31-40 of 141 Abreviews or Q&A

Western blot abreview for Anti-beta Actin antibody [AC-15]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Guinea pig Cell lysate - whole cell (Brain)
Loading amount
30 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Apr 02 2012

Question


Merci de trouver en attaché le fichier word tel que demandé dans votre precedent email.

J'espère que ce formulaire réponse vous sera d'ue grande utilité.

Nous pensons qu'untube de remplacement serait mieux.

Merci de votre cooperation.

J'attends de vos nouvelles.

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Answer

Merci pour votre réponse.

Afin que je puisse vous envoyer le tube de remplacement, il me faux que je trouve la commande originale. Est-ce que vous pouvez me dire soit le numéro de commande soit la date approximative de la commande, avec le nom de la personne qui avait passé la commande? Ceci est un anticorps très populaire, il m'est donc difficile à trouver la commande sans votre aide.

Aussi, vous mentionnez que vous avez optimisé la dilution de l'anticorps. Est-ce que vous vous rappelez quels dilutions vous avez testés? Effectivement, cet anticorps est un ascite et il peux donc avoir des variations de concentrations entre les lots. Nous pouvons donc pas garantir, que tous les lots vont fonctionner à 1/20000.

Merci de votre coopération. Je me réjouis de votre réponse, afin de pouvoir vous envoyer le tube de remplacement.

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Question

Inquiry: Hi I would like to purchase numerous antibodies for my phd work. The products i require are: Ab54481, Ab105468, Ab6721, Ab6726, Ab97240. In your descriptions you say the positive control for Ab54481, Ab105468 is HepG2 whole cell lysate. Could you confirm whether this was achieved with the whole cell lysates that you have in your catalogue and if so which one? or was the control any HepG2 cell lysate? I am looking to order these products tomorrow so your help would be very much appreciated. Kind regards

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Answer

Thank you for contacting us.
The results were achieved by using cell lysates other than are present in catalogue. Any HepG2 cell lysates can be used. We also have these in catalogue as ready to use lysates so you can buy these from our website.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

please check reference: Oscar A. Bizzozero and Jianzheng Zheng Journal of Neuroscience Research 87:2881-2889 (2009) I'm talking about substitute of beta- actin (43kDa) ab-26276 they use in this artical for nitrosylated actin. Hope I'll have correct information this time. Thank you,

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Answer

Thank you for this additional information.

It appears that the information in the referenced article is incorrect. Ab26276 is thediscontinuedAnti-SorLA antibody which does not appear by name in this article. However they do identify an anti beta-actin antibody with this code in the”Pull-Down of S-Nitrosylated Proteins” section:

“β-actin (Ab-26276; Genetex Inc, San Antonio, TX)”

I did look for this at the Genetex Inc. website and did not find this product number. All the Genetex products begin with the prefix GTX-. As the reference number begins with the Abcam AB- prefix it seems likely that this group did use an Abcam product. It is also likely that they used ab6276which is ourAnti-beta Actin antibody [AC-15] and has a very similar code. We currently carry this product which has been tested in a large number of applications and species and has over 200 specific references. The link to this product is below:

https://www.abcam.com/beta-Actin-antibody-AC-15-ab6276.html

While I am fairly confident that ab6276 is the product which you have been looking for, as there are two mistakes, product number and company, I would suggest contacting the authors of the paper to ensure that this is correct.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.


Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

Thank you for your answer. I will order the antibody with reference number AB49856 (mouse anti-beta-actin). Today I will send that information for my secretary services and probably tomorrow they order to you. Can you estimate how long it takes to have the product in my lab?

Thanks in advance,

Yours sincerely,

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Answer

Thank you for your answer and interest in our antibody.

The ab49856 is in stock and will arrive within 48 hours at your place when we receive the order.

Please do not hesitate to contact us, should you need any other information.

I wish you a great day!

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Question

Dear Sir/Madam

I am a phD student from xx and I am interested in order an antibody anti-beta actin. I realized that you have two similar antibodies against that protein, so I would like to know principal differences between them: ref. #ab6276 and #49846.

I am interested in use it for immunocytochemistry in neuronal cells (cell cultures). So, I would like to ask if it was already tested in this kind of cells.

Thank you in advance.

Yours sincerely,

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Answer

Thank you very much for your inquiry.

I can confirm that this is the same clone. So the antibody itself is the same. The main difference between these two products are, that ab6276 is unpurified Ascite whereas ab49846 is purified. Both versions have however been tested in ICC. In order to find whether they have been tested in ICC on neuronal cells, I can recommend to look through the different publications we have for the ab6276: https://www.abcam.com/beta-Actin-antibody-AC-15-ab6276-references.html

I can also confirm, that we do guarantee all our products to work as stated on the datasheet. We do therefore guarantee ab6276 and ab49846 to work in ICC.

I hope this information helps. Please do not hesitate to contact us again, should you have any other question.

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Question

I have a problem with the antibody ab6276. I try to develop an indirect ELISA with this antibody, but do not get any result. This is the procedure, I've performed: - Coating of ELISA plate (Costar high bind) with actin (rabbit skeletal muscle, Cytoskeleton, inc. # AKL95)in carbonate/ bicarbonate buffer pH 9.6. Concentration range: 0.625 - 10 µg/mL), coating for 1h at room temperature or over night at 4 ˚C - Blocking: 5 % milk in PBST, 1h at room temperature - Detection antibody (ab6276): dilution in blocking buffer (see above), incubation for 1 h at room temp, dilution: 1:500 - 1:4000 - Secondary antibody (goat anti-mouse IgG HRP, santa cruz biotechnology, # sc-2005), dilution in blocking buffer (see above), incubation for 1 h at room temp, dilution: 1:2000 - 1:8000 - Detection with TMB substrate. No color development, also not after 2 hours. Between all steps, the plate was washed 3x with TBST (200 µL wash buffer/ well) In order to find the problem, I did the following tests: Antigen adsorption study with BCA assay. Antigen did adsorb on surface with a concentration of about 1 µg/mL. Adsorption of secondary antibody on plate, detection with secondary antibody: positive, ABs react with each other. Test of secondary AB with substrate: positive. Increasing the secondary antibody concentration to 1:1000, color development was detected, but the negative control (no antigen) showed the same intensity like the samples. SDS PAGE/ Western blot with actin from the same source: positive. Do you have any suggestions? My thought is, that the protein is maybe folded in a way, that the antibody can't bind to the epitope since it is able to bind to the denatured protein in the western blot.

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Answer

Thank you for contacting us.

I would not recommend adding SDS or a reducing agent to the sample prior to coating, as this may interfere with the coating efficiency. It is possible that by boiling the protein for 5-10 minutes, the heat denaturation may be enough to expose the epitope that is being recognized by this antibody in WB.

This antibody is designed for detection of beta actin, not skeletal muscle actin. The homology between rabbit skeletal muscle actin (http://www.uniprot.org/uniprot/P68135)and the immunogen for this antibody is only 64%. Using an antibody directed against a more conserved region of actin, such as ab11003, may give better results. This antibody shows 100% homology with the above mentioned rabbit sequence.

I hope this helps, please let me know if you need any additional information or assistance.

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Question

no signal with the new vial

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Answer

Je suis désolée d'apprendre que le nouveautubede l'ab6276 ne vous donne pas de bons résultats.

Comme discuté au téléphone, nous allons vous remplacer ce tube en accord avec notre garantie. Je vous serais toute fois reconnaissante, si vous pouvez prendre 5 minutes pour nous remplir le questionnaire pour notre suivi de qualité. Je vous ai mis notre questionnaire en document attaché.

Veuillez me confirmer s'il vous plaît aussi le numéro de commande ou la date de commande avec le nom de la personne qui avait passé la commande. Ceci va me permettre à identifier le tube et arranger le remplacement. Veuillez aussi me confirmer si vous souhaitez un tube de remplacement ou un avoir/remboursement.

Je vous remercie par avance de nous envoyer votre protocoleetles autres détails.

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Question

I have a question about the epitope, ab6276 is directed against. In the data sheet is written, that the immunogen is a synthetic peptide (DDDIAALVIDNGSGL), which corresponds to the amino acids 1-15 of Xenopus laevis beta-actin. When I was looking on this amino acid sequence, I did not find the epitope, but I found EDDIAALVVDNGSGM, which is equal except 3 amino acids. But if I try to find this sequence in the human actin, I just find a 44.4% identity in 9 residues overlap 80.0% identity in 5 residues overlap 18.2% identity in 11 residues overlap 75.0% identity in 4 residues overlap and so on. My question is: Where should the epitope in human beta actin be located? Where is it meant to bind to? Or does it bind to any of these epitopes? I ordered this antibody, since it was directed against aa 1-15, and I wanted to have an antibody which is directed against an epitope at the C-terminal end for a sandwich ELISA. The capture antibody I use, is ab34731, which recognizes an epitope at the N-terminal end. If ab6276 reacts with an epitope in the middle of the protein, it might be useless for me.

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Answer

Thank you for contacting us.

The antibody ab6276 is raised against the peptide specified on the datasheet. You are correct, it does not correspond exactly to the X. laevis sequence, and we will very likely remove this note about X. laevis. Thank you for bringing this to our attention.

Reactivity with human beta actin has been demonstrated many times, though the exact location of the epitope that the binding site of the antibody recognizes on the immunogen sequence is not known. Alignment with the human sequence given at the UniProt entry at the following URL shows 87% identity (13/15):

http://www.uniprot.org/uniprot/P60709

Please do not hesitate to contact us if you have any more questions.

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Question

Please suggest secondary antibodies for ab15571 and ab6276.
Using on rat samples, ICC-IF in double staining.

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Answer

Thank you for your telephone enquiry yesterday.

As requested, I am forwarding a list of suitable secondary antibodies for use with ab15571 and ab6276. These have been tested and guaranteed in ICC-IF. I have selected some FITC and PE conjugated antibodies. I can recommend to review the online datasheets for further information.

For the double staining, you will need to select an FITC conjugated secondary for one of the primaries, and a PE conjugated secondary for the other. I have provided a selection of both below, so you can make your own choice.

Secondaries for use with ab6276 mouse IgG1:

FITC conjugated:

ab97239 Goat anti-Mouse IgG1 heavy chain (FITC) secondary antibody
Tested in: Flow Cyt, ICC/IF, IHC-P
https://www.abcam.com/Goat-Mouse-IgG1-heavy-chain-FITC-ab97239.html (or use the following: https://www.abcam.com/Goat-Mouse-IgG1-heavy-chain-FITC-ab97239.html).

ab98692 Goat polyclonal Secondary Antibody to Mouse IgG1 - heavy chain (FITC), pre-adsorbed
Tested in: Flow Cyt, ICC/IF, IHC-P
https://www.abcam.com/Goat-Mouse-IgG1-heavy-chain-FITC-preadsorbed-ab98692.html (or use the following: https://www.abcam.com/Goat-Mouse-IgG1-heavy-chain-FITC-preadsorbed-ab98692.html).

PE conjugated:

ab99605 Rat monoclonal [SB77e] Secondary Antibody to Mouse IgG1 (PE), pre-adsorbed
Tested in: ELISA, Flow Cyt, ICC/IF
https://www.abcam.com/Rat-monoclonal-SB77e-Mouse-IgG1-HL-PE-preadsorbed-ab99605.html (or use the following: https://www.abcam.com/Rat-monoclonal-SB77e-Mouse-IgG1-HL-PE-preadsorbed-ab99605.html).


Secondaries for use with ab15571 Rabbit polyclonal:

FITC conjugated:

ab97050 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC)
Tested in: Flow Cyt, ICC/IF, IHC-P
https://www.abcam.com/Goat-Rabbit-IgG-HL-FITC-ab97050.html (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-HL-FITC-ab97050.html).

ab97199 Goat polyclonal Secondary Antibody to Rabbit IgG - Fc (FITC)
Tested in: Flow Cyt, ICC/IF, IHC-P
https://www.abcam.com/Goat-Rabbit-IgG-Fc-FITC-ab97199.html (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-Fc-FITC-ab97199.html).

PE conjugated:

ab7007 Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (PE), pre-adsorbed
Tested in: Flow Cyt, ICC/IF, IHC-P, IM
https://www.abcam.com/Donkey-Fab2-Rabbit-IgG-HL-PE-preadsorbed-ab7007.html (or use the following: https://www.abcam.com/Donkey-Fab2-Rabbit-IgG-HL-PE-preadsorbed-ab7007.html).

ab50677
Goat anti-Rabbit IgG H&L (PE) secondary antibody
Tested in: ICC-IF.
https://www.abcam.com/Goat-Rabbit-IgG-HL-PE-preadsorbed-ab50677.html (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-HL-PE-preadsorbed-ab50677.html).

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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31-40 of 141 Abreviews or Q&A

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