Recombinant Anti-beta Actin antibody [EPR21242] (ab241153)
![Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [EPR21242] (ab241153)](https://www.abcam.com/ps/products/241/ab241153/Images/ab241153-320190-Ab241153IGX6179RAR8305nih3t3mjpg.jpg "Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [EPR21242] (ab241153)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21242] to beta Actin
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Mouse, Human
Overview
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Product name
Anti-beta Actin antibody [EPR21242]
See all beta Actin primary antibodies -
Description
Rabbit monoclonal [EPR21242] to beta Actin -
Host species
Rabbit -
Specificity
In-house testing showed ab241153 to react with gamma actin in western blot.
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Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: Methanol-fixed HeLa and NIH3T3 cells. WB: HAP1, MEF1 and NIH3T3 whole cell lysates
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General notes
This product was made using synthetic libraries and phage display technology.
This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR21242 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
- Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab241153 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa.
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| ICC/IF |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa. |
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ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
Involvement in disease
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. -
Sequence similarities
Belongs to the actin family. -
Post-translational
modificationsISGylated. -
Cellular localization
Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Omim: 102630 Human
- SwissProt: P60709 Human
- SwissProt: P60710 Mouse
- Unigene: 520640 Human
- Unigene: 708120 Human
- Unigene: 727576 Human
see all -
Alternative names
- A26C1A antibody
- A26C1B antibody
- ACTB antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [EPR21242] (ab241153)
ab213243 staining beta-Actin in NIH3T3 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab241153 at 0.5ugml then detected with an Alexa Fluor® 488 goat anti-rabbit secondary antibody (ab150081) at a 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue), and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Western blot - Anti-beta Actin antibody [EPR21242] (ab241153)](https://www.abcam.com/ps/products/241/ab241153/Images/ab241153-337173-ab241153AP4444149.jpg)
Western blot - Anti-beta Actin antibody [EPR21242] (ab241153)All lanes :
Lane 1 : HAP1 whole cell lysate at 20 µg
Lane 2 : HAP1 ACTNB whole cell lysate at 20 µg
Lane 3 : NIH3T3 whole cell lysate at 20 µg
Lane 4 : MEF whole cell lysate at 20 µg
Lane 5 :Recombinant Human gamma Actin protein (ab157841)
Performed under reducing conditions.
Predicted band size: 42 kDaab241153 was shown to react with ACTNB (beta actin) when tested against HAP1 wild-type and HAP1 ACTNB (beta actin) knockout cells. ACTNB wild-type and knockout samples were subjected to SDS-PAGE and incubated with ab231153. Incomplete reduction of signal was seen in the ACTNB (beta actin) knockout samples at beta actin's expected molecular weight. SDS-PAGE of gamma actin recombinant protein and incubation with ab241153 showed cross-reactivity to gamma actin.
ab241153 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at a 1ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [EPR21242] (ab241153)](https://www.abcam.com/ps/products/241/ab241153/Images/ab241153-320189-Ab241153IGX6179RAR8305nih3t3m.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [EPR21242] (ab241153)ab213243 staining beta-Actin in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab241153 at 0.01ugml then detected with an Alexa Fluor® 488 goat anti-rabbit secondary antibody (ab150081) at a 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue), and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Anti-beta Actin antibody [EPR21242] (ab241153)](https://www.abcam.com/ps/products/241/ab241153/Images/ab241153-4-benefits-of-recombinant-antibodies.png)
Anti-beta Actin antibody [EPR21242] (ab241153)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab241153 has not yet been referenced specifically in any publications.