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RecombinantRabMAb

Recombinant Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

Reviews (1) Submit a question References (67)
Western blot - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
  • Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
  • Flow Cytometry (Intracellular) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
  • Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
  • Flow Cytometry (Intracellular) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
  • Flow Cytometry (Intracellular) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
  • Western blot - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
  • Flow Cytometry (Intracellular) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
  • Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP124] to beta Actin - Cytoskeleton Marker
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free

Overview

  • Product name

    Anti-beta Actin antibody [SP124] - Cytoskeleton Marker
    See all beta Actin primary antibodies

  • Description

    Rabbit monoclonal [SP124] to beta Actin - Cytoskeleton Marker

  • Host species

    Rabbit

  • Specificity

    The immunogen used for this product shares 71% homology with Gamma actin/actin cytoplasmic 2 and 64% homology with cardiac, smooth, and skeletal muscle actin. Cross-reactivity with these proteins has not been confirmed experimentally.

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Chicken, Cow, Dog, Pig

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human Placenta, Human placenta, Mouse placenta, and Rat placenta tissue; WB: Raji, HeLa, A549, HEK-293, NIH/3T3 and Raw 264.7 cell lystate; Flow Cyt: HeLa, NIH/3T3, and C6 cells; ICC/IF: NIH/3T3, and C6 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab115777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/200. Predicted molecular weight: 42 kDa.
IHC-P
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
ICC/IF
1/50.
Flow Cyt
1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Notes
WB
1/200. Predicted molecular weight: 42 kDa.
IHC-P
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
ICC/IF
1/50.
Flow Cyt
1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

Images

  • Western blot - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
    Western blot - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
    All lanes : Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777) at 1/200 dilution

    Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
    Lane 4 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
    Lane 5 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 6 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 10 seconds


    Blocking/Diluting buffer and concentration 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat placenta tissue sections labeling beta Actin with ab115777 at 1/100 dilution. Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with citrate sodium buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse placenta tissue sections labeling beta Actin with ab115777 at 1/200 dilution. Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with citrate sodium buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human placenta tissue sections labeling beta Actin with ab115777 at 1/200 dilution. Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with citrate sodium buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
    Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

    Immunocytochemistry/ Immunofluorescence analysis of C6 (rat glial tumor glial cell) cells labeling beta Actin with purified ab115777 at 1/50 dilutioin. Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry (Intracellular) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
    Flow Cytometry (Intracellular) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

    Flow Cytometry analysis of C6 (rat glial tumor glial cell) cells labeling beta Actin with purified ab115777 at 1/200 - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) was used as the secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
    Staining of Human beta Actin in a Formalin/PFA-fixed paraffin-embedded section of Human Placenta using ab115777 at a dilution of 1/200.
  • Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
    Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

    Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 (mouse embryonic fibroblast) cells labeling beta Actin with purified ab115777 at 1/50 dilution. Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry (Intracellular) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
    Flow Cytometry (Intracellular) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

    Flow Cytometry analysis of NIH/3T3 (mouse embryonic fibroblast) cells labeling beta Actin with purified ab115777 at 1/200 - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) was used secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.

  • Flow Cytometry (Intracellular) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
    Flow Cytometry (Intracellular) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

    Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling beta Actin with purified ab115777 at 1/200 - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081)  was used as the secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.

  • Western blot - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
    Western blot - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
    Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777) at 1/200 dilution + Raji cell lystate

    Predicted band size: 42 kDa

  • Flow Cytometry (Intracellular) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
    Flow Cytometry (Intracellular) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

    Flow cytometric analysis of rabbit anti-beta Actin (SP124) antibody ab115777 (1/200) in HeLa cells (green) compared to negative control of rabbit IgG (blue)

  • Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
    Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

References (67)

Publishing research using ab115777? Please let us know so that we can cite the reference in this datasheet.

ab115777 has been referenced in 67 publications.

  • Yang X  et al. Rs41291957 polymorphism in the promoter region of microRNA-143 serves as a prognostic biomarker for patients with intracranial hemorrhage. Mol Med Rep 23:N/A (2021). PubMed: 33649782
  • Wang G  et al. CTRP12 ameliorates atherosclerosis by promoting cholesterol efflux and inhibiting inflammatory response via the miR-155-5p/LXRa pathway. Cell Death Dis 12:254 (2021). PubMed: 33692340
  • Feng Y  et al. Dl-3-n-Butylphthalide Alleviates Demyelination and Improves Cognitive Function by Promoting Mitochondrial Dynamics in White Matter Lesions. Front Aging Neurosci 13:632374 (2021). PubMed: 33762923
  • Li N  et al. Upregulation of deubiquitinase USP7 by transcription factor FOXO6 promotes EC progression via targeting the JMJD3/CLU axis. Mol Ther Oncolytics 20:583-595 (2021). PubMed: 33768140
  • Luo P  et al. S-Allylmercaptocysteine improves alcoholic liver disease partly through a direct modulation of insulin receptor signaling. Acta Pharm Sin B 11:668-679 (2021). PubMed: 33777674
View all Publications for this product

Customer reviews and Q&As

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Western blot abreview for Anti-beta Actin antibody [SP124] - Cytoskeleton Marker

 Good
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (cardiac muscle)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
20 µg
Specification
cardiac muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Nov 17 2021

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com