Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
RecombinantRabMAb

Recombinant Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

Reviews (1) Submit a question References (105)
Western blot - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
  • Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
  • Flow Cytometry (Intracellular) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
  • Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
  • Flow Cytometry (Intracellular) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
  • Flow Cytometry (Intracellular) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
  • Western blot - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
  • Flow Cytometry (Intracellular) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
  • Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP124] to beta Actin - Cytoskeleton Marker
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free

Overview

  • Product name

    Anti-beta Actin antibody [SP124] - Cytoskeleton Marker
    See all beta Actin primary antibodies

  • Description

    Rabbit monoclonal [SP124] to beta Actin - Cytoskeleton Marker

  • Host species

    Rabbit

  • Specificity

    The immunogen used for this product shares 71% homology with Gamma actin/actin cytoplasmic 2 and 64% homology with cardiac, smooth, and skeletal muscle actin. Cross-reactivity with these proteins has not been confirmed experimentally.

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Chicken, Cow, Dog, Pig

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human Placenta, Human placenta, Mouse placenta, and Rat placenta tissue; WB: Raji, HeLa, A549, HEK-293, NIH/3T3 and Raw 264.7 cell lystate; Flow Cyt: HeLa, NIH/3T3, and C6 cells; ICC/IF: NIH/3T3, and C6 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab115777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/200. Predicted molecular weight: 42 kDa.
IHC-P
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
ICC/IF
1/50.
Flow Cyt (Intra)
1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Notes
WB
1/200. Predicted molecular weight: 42 kDa.
IHC-P
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
ICC/IF
1/50.
Flow Cyt (Intra)
1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

Images

  • Western blot - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
    Western blot - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
    All lanes : Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777) at 1/200 dilution

    Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
    Lane 4 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
    Lane 5 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 6 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 10 seconds


    Blocking/Diluting buffer and concentration 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat placenta tissue sections labeling beta Actin with ab115777 at 1/100 dilution. Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with citrate sodium buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse placenta tissue sections labeling beta Actin with ab115777 at 1/200 dilution. Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with citrate sodium buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human placenta tissue sections labeling beta Actin with ab115777 at 1/200 dilution. Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with citrate sodium buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
    Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

    Immunocytochemistry/ Immunofluorescence analysis of C6 (rat glial tumor glial cell) cells labeling beta Actin with purified ab115777 at 1/50 dilutioin. Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry (Intracellular) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
    Flow Cytometry (Intracellular) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

    Flow Cytometry analysis of C6 (rat glial tumor glial cell) cells labeling beta Actin with purified ab115777 at 1/200 - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) was used as the secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
    Staining of Human beta Actin in a Formalin/PFA-fixed paraffin-embedded section of Human Placenta using ab115777 at a dilution of 1/200.
  • Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
    Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

    Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 (mouse embryonic fibroblast) cells labeling beta Actin with purified ab115777 at 1/50 dilution. Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry (Intracellular) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
    Flow Cytometry (Intracellular) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

    Flow Cytometry analysis of NIH/3T3 (mouse embryonic fibroblast) cells labeling beta Actin with purified ab115777 at 1/200 - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) was used secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.

  • Flow Cytometry (Intracellular) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
    Flow Cytometry (Intracellular) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

    Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling beta Actin with purified ab115777 at 1/200 - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081)  was used as the secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.

  • Western blot - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
    Western blot - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
    Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777) at 1/200 dilution + Raji cell lystate

    Predicted band size: 42 kDa

  • Flow Cytometry (Intracellular) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
    Flow Cytometry (Intracellular) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

    Flow cytometric analysis of rabbit anti-beta Actin (SP124) antibody ab115777 (1/200) in HeLa cells (green) compared to negative control of rabbit IgG (blue)

  • Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
    Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)

References (105)

Publishing research using ab115777? Please let us know so that we can cite the reference in this datasheet.

ab115777 has been referenced in 105 publications.

  • Wu R  et al. Hsa_circ_0003602 Contributes to the Progression of Colorectal Cancer by Mediating the miR-149-5p/SLC38A1 Axis. Gut Liver 17:267-279 (2023). PubMed: 36148577
  • Ma H & Ma Y Dapagliflozin Inhibits Ventricular Remodeling in Heart Failure Rats by Activating Autophagy through AMPK/mTOR Pathway. Comput Math Methods Med 2022:6260202 (2022). PubMed: 36193200
  • Jiang M  et al. Curcumin Reprograms TAMs from a Protumor Phenotype towards an Antitumor Phenotype via Inhibiting MAO-A/STAT6 Pathway. Cells 11:N/A (2022). PubMed: 36359867
  • Wang M  et al. Isorhamnetin inhibits progression of ovarian cancer by targeting ESR1. Ann Transl Med 10:1216 (2022). PubMed: 36544694
  • Huang Z  et al. circFIG 4 drives the carcinogenesis and metastasis of esophagus cancer via the miR-493-5p/E2F3 axis. Thorac Cancer 13:783-794 (2022). PubMed: 35083866
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Western blot abreview for Anti-beta Actin antibody [SP124] - Cytoskeleton Marker

 Good
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (cardiac muscle)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
20 µg
Specification
cardiac muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 17 2021

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com