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  1. Link

    products/primary-antibodies/beta-amyloid-antibody-de2b4-ab11132.pdf

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Neuroscience Neurology process Neurodegenerative disease Alzheimer's disease Amyloid
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Anti-beta Amyloid antibody [DE2B4] (ab11132)

  • Datasheet
  • SDS
Reviews (8)Q&A (4)References (30)

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Immunohistochemistry (Frozen sections) - Anti-beta Amyloid antibody [DE2B4] (ab11132)
  • Immunocytochemistry/ Immunofluorescence - Anti-beta Amyloid antibody [DE2B4] (ab11132)

Key features and details

  • Mouse monoclonal [DE2B4] to beta Amyloid
  • Suitable for: ICC/IF, IP, IHC-Fr, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-beta Amyloid antibody [DE2B4]
    See all beta Amyloid primary antibodies
  • Description

    Mouse monoclonal [DE2B4] to beta Amyloid
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, IP, IHC-Fr, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 1-17 of Human beta Amyloid.

  • Epitope

    ab11132 recognizes an epitope in the region 8-16 of the amyloid precursor protein.
  • Positive control

    • IHC-P: Alzheimer's disease human brain; Mouse brain tissue. IHC-Fr: Mouse brain cortex. WB: CHO-K1 cell lysate. ICC/IF: HEK cells.
  • General notes

    This product should be stored undiluted. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    DE2B4
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Amyloid
    • Neuroscience
    • Diseases

Associated products

  • Alternative Versions

    • HRP Anti-beta Amyloid antibody [DE2B4] (ab187908)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab11132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (1)
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
IHC-Fr (4)
Use at an assay dependent concentration.
IHC-P (3)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 87 kDa. PubMed: 19955183
Notes
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 87 kDa. PubMed: 19955183

Target

  • Function

    Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity (By similarity). Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu(2+)-mediated low-density lipoprotein oxidation. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Induces a AGER-dependent pathway that involves activation of p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons.
    Beta-amyloid peptides are lipophilic metal chelators with metal-reducing activity. Bind transient metals such as copper, zinc and iron. In vitro, can reduce Cu(2+) and Fe(3+) to Cu(+) and Fe(2+), respectively. Beta-amyloid 42 is a more effective reductant than beta-amyloid 40. Beta-amyloid peptides bind to lipoproteins and apolipoproteins E and J in the CSF and to HDL particles in plasma, inhibiting metal-catalyzed oxidation of lipoproteins. Beta-APP42 may activate mononuclear phagocytes in the brain and elicit inflammatory responses. Promotes both tau aggregation and TPK II-mediated phosphorylation. Interaction with overexpressed HADH2 leads to oxidative stress and neurotoxicity.
    Appicans elicit adhesion of neural cells to the extracellular matrix and may regulate neurite outgrowth in the brain.
    The gamma-CTF peptides as well as the caspase-cleaved peptides, including C31, are potent enhancers of neuronal apoptosis.
    N-APP binds TNFRSF21 triggering caspase activation and degeneration of both neuronal cell bodies (via caspase-3) and axons (via caspase-6).
  • Tissue specificity

    Expressed in all fetal tissues examined with highest levels in brain, kidney, heart and spleen. Weak expression in liver. In adult brain, highest expression found in the frontal lobe of the cortex and in the anterior perisylvian cortex-opercular gyri. Moderate expression in the cerebellar cortex, the posterior perisylvian cortex-opercular gyri and the temporal associated cortex. Weak expression found in the striate, extra-striate and motor cortices. Expressed in cerebrospinal fluid, and plasma. Isoform APP695 is the predominant form in neuronal tissue, isoform APP751 and isoform APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Appican is expressed in astrocytes.
  • Involvement in disease

    Defects in APP are the cause of Alzheimer disease type 1 (AD1) [MIM:104300]. AD1 is a familial early-onset form of Alzheimer disease. It can be associated with cerebral amyloid angiopathy. Alzheimer disease is a neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death.
    Defects in APP are the cause of amyloidosis cerebroarterial Dutch type (AMYLCAD) [MIM:605714]; also known as hereditary cerebral hemorrhage with amyloidosis Dutch type (HCHWAD). AMYLCAD is a hereditary localized amyloidosis due to amyloid-beta A4 peptide(s) deposition in the cerebral vessels. Beta-APP40 is the predominant form of cerebrovascular amyloid. Amyloid is not found outside the nervous system. The principal clinical characteristics are recurrent cerebral and cerebellar hemorrhages, recurrent strokes, cerebral ischemia, cerebral infarction, and progressive mental deterioration. Onset of the disease is in middle age (44 to 60 years). Patients develop cerebral hemorrhage because of the severe cerebral amyloid angiopathy. Parenchymal amyloid deposits are rare and largely in the form of pre-amyloid lesions or diffuse plaque-like structures. They are Congo red negative and lack the dense amyloid cores commonly present in Alzheimer disease.
    Defects in APP are the cause of amyloidosis cerebroarterial Italian type (AMYLCAIT) [MIM:605714]. AMYLCAIT is a hereditary localized amyloidosis due to amyloid-beta A4 peptide(s) deposition in the cerebral vessels, resulting in cerebral amyloid angiopathy. Amyloid is not found outside the nervous system. It is a condition very similar to AMYLCAD, but the clinical course is less severe. Patients manifest mild cognitive decline, recurrent strokes, and epilepsy in some cases. There are extensive amyloid deposits in leptomeningeal and cortical vessels and, to a lesser extent, in the neuropil of the cerebral cortex, in the absence of neurofibrillary tangles.
    Defects in APP are the cause of amyloidosis cerebroarterial Iowa type (AMYLCAIW) [MIM:605714]. AMYLCAIW is a hereditary amyloidosis due to amyloid-beta A4 peptide(s) deposition. Patients have progressive aphasic dementia, leukoencephalopathy, and occipital calcifications.
  • Sequence similarities

    Belongs to the APP family.
    Contains 1 BPTI/Kunitz inhibitor domain.
  • Domain

    The basolateral sorting signal (BaSS) is required for sorting of membrane proteins to the basolateral surface of epithelial cells.
    The NPXY sequence motif found in many tyrosine-phosphorylated proteins is required for the specific binding of the PID domain. However, additional amino acids either N- or C-terminal to the NPXY motif are often required for complete interaction. The PID domain-containing proteins which bind APP require the YENPTY motif for full interaction. These interactions are independent of phosphorylation on the terminal tyrosine residue. The NPXY site is also involved in clathrin-mediated endocytosis.
  • Post-translational
    modifications

    Proteolytically processed under normal cellular conditions. Cleavage either by alpha-secretase, beta-secretase or theta-secretase leads to generation and extracellular release of soluble APP peptides, S-APP-alpha and S-APP-beta, and the retention of corresponding membrane-anchored C-terminal fragments, C80, C83 and C99. Subsequent processing of C80 and C83 by gamma-secretase yields P3 peptides. This is the major secretory pathway and is non-amyloidogenic. Alternatively, presenilin/nicastrin-mediated gamma-secretase processing of C99 releases the amyloid beta proteins, amyloid-beta 40 (Abeta40) and amyloid-beta 42 (Abeta42), major components of amyloid plaques, and the cytotoxic C-terminal fragments, gamma-CTF(50), gamma-CTF(57) and gamma-CTF(59).
    Proteolytically cleaved by caspases during neuronal apoptosis. Cleavage at Asp-739 by either caspase-6, -8 or -9 results in the production of the neurotoxic C31 peptide and the increased production of beta-amyloid peptides.
    N- and O-glycosylated. O-linkage of chondroitin sulfate to the L-APP isoforms produces the APP proteoglycan core proteins, the appicans. The chondroitin sulfate chain of appicans contains 4-O-sulfated galactose in the linkage region and chondroitin sulfate E in the repeated disaccharide region.
    Phosphorylation in the C-terminal on tyrosine, threonine and serine residues is neuron-specific. Phosphorylation can affect APP processing, neuronal differentiation and interaction with other proteins. Phosphorylated on Thr-743 in neuronal cells by Cdc5 kinase and Mapk10, in dividing cells by Cdc2 kinase in a cell-cycle dependent manner with maximal levels at the G2/M phase and, in vitro, by GSK-3-beta. The Thr-743 phosphorylated form causes a conformational change which reduces binding of Fe65 family members. Phosphorylation on Tyr-757 is required for SHC binding. Phosphorylated in the extracellular domain by casein kinases on both soluble and membrane-bound APP. This phosphorylation is inhibited by heparin.
    Extracellular binding and reduction of copper, results in a corresponding oxidation of Cys-144 and Cys-158, and the formation of a disulfide bond. In vitro, the APP-Cu(+) complex in the presence of hydrogen peroxide results in an increased production of beta-amyloid-containing peptides.
    Trophic-factor deprivation triggers the cleavage of surface APP by beta-secretase to release sAPP-beta which is further cleaved to release an N-terminal fragment of APP (N-APP).
    Beta-amyloid peptides are degraded by IDE.
  • Cellular localization

    Membrane. Membrane > clathrin-coated pit. Cell surface protein that rapidly becomes internalized via clathrin-coated pits. During maturation, the immature APP (N-glycosylated in the endoplasmic reticulum) moves to the Golgi complex where complete maturation occurs (O-glycosylated and sulfated). After alpha-secretase cleavage, soluble APP is released into the extracellular space and the C-terminal is internalized to endosomes and lysosomes. Some APP accumulates in secretory transport vesicles leaving the late Golgi compartment and returns to the cell surface. Gamma-CTF(59) peptide is located to both the cytoplasm and nuclei of neurons. It can be translocated to the nucleus through association with APBB1 (Fe65). Beta-APP42 associates with FRPL1 at the cell surface and the complex is then rapidly internalized. APP sorts to the basolateral surface in epithelial cells. During neuronal differentiation, the Thr-743 phosphorylated form is located mainly in growth cones, moderately in neurites and sparingly in the cell body. Casein kinase phosphorylation can occur either at the cell surface or within a post-Golgi compartment.
  • Target information above from: UniProt accession P05067 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 351 Human
    • Omim: 104760 Human
    • SwissProt: P05067 Human
    • Unigene: 434980 Human
    • Alternative names

      • A4_HUMAN antibody
      • AAA antibody
      • ABETA antibody
      • ABPP antibody
      • AD1 antibody
      • AICD-50 antibody
      • AICD-57 antibody
      • AICD-59 antibody
      • AID(50) antibody
      • AID(57) antibody
      • AID(59) antibody
      • Alzheimer disease amyloid protein antibody
      • amyloid beta A4 protein antibody
      • Amyloid intracellular domain 50 antibody
      • Amyloid intracellular domain 57 antibody
      • Amyloid intracellular domain 59 antibody
      • amyloid of aging and alzheimer disease antibody
      • APP antibody
      • APPI antibody
      • beta-amyloid peptide antibody
      • Beta-APP40 antibody
      • Beta-APP42 antibody
      • C31 antibody
      • Cerebral vascular amyloid peptide antibody
      • CTFgamma antibody
      • CVAP antibody
      • Gamma-CTF(50) antibody
      • Gamma-CTF(57) antibody
      • Gamma-CTF(59) antibody
      • peptidase nexin-II antibody
      • PN-II antibody
      • PreA4 antibody
      • Protease nexin-II antibody
      • S-APP-alpha antibody
      • S-APP-beta antibody
      see all

    Images

    • Immunohistochemistry (Frozen sections) - Anti-beta Amyloid antibody [DE2B4] (ab11132)
      Immunohistochemistry (Frozen sections) - Anti-beta Amyloid antibody [DE2B4] (ab11132)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom

      ab11132 staining beta Amyloid in transgenic APPP23 mouse brain cortex by immunohistochemistry (frozen sections). Unfixed cryosections were fixed in 4% Formalin in PBS before treatment with 70% Formic acid to reveal antigenic sites. Fixed samples were permeabilized with 0.1% Tween 20, blocked with 1% BSA for 30 minutes at 1oC. Samples were incubated with primary antibody (1/200 in TBS/BSA/Tween 20/azide) for 2 hours. Anti-mouse IgG Alexa Fluor® 594 (1/1000) was used as the secondary antibody.

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-beta Amyloid antibody [DE2B4] (ab11132)
      Immunocytochemistry/ Immunofluorescence - Anti-beta Amyloid antibody [DE2B4] (ab11132)This image is courtesy of Randal Moldrich, CNRS UMR7637, ESPCI, France
      Immunofluorescence detection using Mouse monoclonal [DE2B4] to beta Amyloid (ab11132) on Human HEK cells. Cells were fixed using Methanol, no antigen retrieval step was necessary. Primary antibody ab11132 was incubated for 1hr at room temperature (1/400). The confocal image of transfected HEK cells shows A. anti-amyloid beta (ab11132), B. swAPP-GFP, C. Overlay. Significant colocalisation is seen as would be expected. (GFP is tagged to C-term of swAPP).

    Protocols

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (30)

    Publishing research using ab11132? Please let us know so that we can cite the reference in this datasheet.

    ab11132 has been referenced in 30 publications.

    • Zhang XM  et al. Therapeutic potential of dental pulp stem cell transplantation in a rat model of Alzheimer's disease. Neural Regen Res 16:893-898 (2021). PubMed: 33229725
    • Bengoa-Vergniory N  et al. Tau-proximity ligation assay reveals extensive previously undetected pathology prior to neurofibrillary tangles in preclinical Alzheimer's disease. Acta Neuropathol Commun 9:18 (2021). PubMed: 33509301
    • Oizumi H  et al. Fatty Acid-Binding Protein 3 Expression in the Brain and Skin in Human Synucleinopathies. Front Aging Neurosci 13:648982 (2021). PubMed: 33841128
    • Chao FL  et al. Fluoxetine Promotes Hippocampal Oligodendrocyte Maturation and Delays Learning and Memory Decline in APP/PS1 Mice. Front Aging Neurosci 12:627362 (2020). PubMed: 33519426
    • Harper DJ  et al. Retinal analysis of a mouse model of Alzheimer's disease with multicontrast optical coherence tomography. Neurophotonics 7:015006 (2020). PubMed: 32042855
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 12 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-beta Amyloid antibody [DE2B4]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (mouse spinal cord)
    Permeabilization
    No
    Specification
    mouse spinal cord
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Yanan Chen Chen

    Verified customer

    Submitted Aug 31 2016

    Immunohistochemistry (Frozen sections) abreview for Anti-beta Amyloid antibody [DE2B4]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Brain)
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Acetone
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mehmet Sedat Feyat

    Verified customer

    Submitted Oct 02 2015

    Immunohistochemistry (Frozen sections) abreview for Anti-beta Amyloid antibody [DE2B4]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (PDAPP (J20) transgenic hAPP-Swe mouse brain)
    Specification
    PDAPP (J20) transgenic hAPP-Swe mouse brain
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.2% Triton X-100
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Barney Bryson

    Verified customer

    Submitted Feb 11 2011

    Immunohistochemistry (Frozen sections) abreview for Anti-beta Amyloid antibody [DE2B4]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (transgenic APP23 mouse brain cortex)
    Specification
    transgenic APP23 mouse brain cortex
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.1% Tween 20
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Carl Hobbs

    Verified customer

    Submitted Apr 19 2010

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-beta Amyloid antibody [DE2B4]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (15 month TASTPM mouse brain)
    Specification
    15 month TASTPM mouse brain
    Fixative
    Formaldehyde
    Antigen retrieval step
    Other
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Carl Hobbs

    Verified customer

    Submitted Jun 29 2009

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-beta Amyloid antibody [DE2B4]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (brain)
    Specification
    brain
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate, 100C , 20 minutes
    Permeabilization
    No
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Ronald Houston

    Verified customer

    Submitted Apr 27 2009

    Question

    No bands in Western blot using these antibodies.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 25 2012

    Answer

    Thank you very much for your call today and for letting us know about the trouble with these antibodies.

    I'msending a free of charge vial of ab3341 on the order *** which should arrive tomorrow, andI'm also sending a vial of ab129097 on the order *** which we expect to arrive around Thursday of next week. Please keep me updated about how these replacement antibodies work for you.

    As we discussed, since ab11132 has not been tested in Western blot in our labs, we can not guarantee that it works in Western blot. So, I have created the discount code below, which can be redeemed for a free primary antibody after submitting an Abreview of ab11132 in Western blot:

    DISCOUNT CODE: ***
    Expiration date: November 26, 2012

    This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forab11132 in Western blotand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Jul 25 2012

    Question

    : Dear Technical Team! We are going to study expression of APP and beta-Amyloid in some human cell lines. Is there crossreactivity between antibodies ab11132 (Anti-beta Amyloid) and ab15272 (Anti-Amyloid Precursor Protein)? We want to recognize a tranzition - from APP to beta-Amyloid.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 12 2012

    Answer

    Thank you for contacting Abcam.

    We have not directly tested if these antibodies would cross react with each other, but based in the immunogen sequences for these antibodies (which are available on the webpage), then I do not believe they would. Also each antibody targets a slightly different part of the protein.

    If there is anything else I can help you with, please let me know.

    Read More

    Abcam Scientific Support

    Answered on Jul 12 2012

    Question

    I'm pretty new to using antibodies, so I have a really basic question. When diluting an antibody for IHC or ICC, what do you dilute it with. Specifically we are using the anti-COX II ab79393 and anti-amyloid beta ab11132. What buffers or solutions do you recommend for diluting these antibodies?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 07 2012

    Answer

    Thank you for contacting Abcam.


    For IHC and ICC, antibodies are typically diluted in PBS or in a 1-3% BSA in PBS solution. Some researchers will use TBS instead, but either is fine. I have included links below to our IHC and ICC protocols for your reference.


    https://www.abcam.com/index.html?pageconfig=resource&rid=11384


    https://www.abcam.com/index.html?pageconfig=resource&rid=11417


    I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

    Read More

    Abcam Scientific Support

    Answered on Jun 07 2012

    Question

    Dear Technical team,   Our user would like to order ab11132.   Before order he wants to some advice from you. He would like to test IHC in mouse tissue injected human cell. And then he wants to confirm human beta Amyloid. Unfortunately, ab11132 has react with human and mouse.   If he use to ab11132, what is he asked to do for low background by mouse?   Could you please give some advice for our user?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 22 2011

    Answer

    Thank you for contacting us. Beta amyloid is 97% identical in mouse and human, so almost any antibody that recognizes human beta amyloid will also recognize mouse beta amyloid. I do not know of a way to block reactivity of the antibody with mouse beta amyloid without also blocking reactivity with human beta amyloid. The best approach for your customer may be to identify the human cells in the mouse tissue with an antibody that recognizes an antigen only found in human cells. The choice of such an antibody depends on the type of cell that the customer intends to inject. If the cells are fibroblasts, a good choice is an anti-vimentin antibody, clone V9: https://www.abcam.com/Vimentin-antibody-V9-Biotin-ab79032.html They will want to use this biotinylated version, ab79032, followed by an avidin or streptavidin conjugate, to avoid using an anti-mouse IgG secondary. If they are then able to use an image analysis program, they can then highlight just the cells of human origin. I hope this information is helpful to your customer.

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    Abcam Scientific Support

    Answered on Apr 22 2011

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