Anti-beta Galactosidase antibody (ab616)

I am very pleased to hear you would like to accept our offer and testab616 and ab15580in ICE. These codes will give you: 1 free primary antibody per code before the expiration date. To redeem this offer, please submit an Abreview for ICE for each antibody and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

Merci de nous avoir contactés.

J'ai pu voir d'après nos archives que plusieurs utilisateurs ont rencontré des problèmes avec cet anticorps en IHC-P. L'application IHC-P a d'ailleurs été retirée de la liste des applications testées, mais malheureusement pas l'image que vous avez mentionnée; ceci est une erreur de notre part que je vais signaler à mes collègues responsables des fiches techniques.

De plus, nous n’offrons pas d’échantillon gratuit à des fins de tests préliminaires. Cependant, je peux vous recommander d’utiliser l’anticorps ab4761 qui a été testé en IHC-P et est couvert par notre Garantie Abpromise. Notre politique est que si un anticorps ne marche pas comme spécifié dans sa fiche technique (espèces et applications), nous offrons un remboursement ou un remplacement gratuit (https://www.abcam.com/abpromise).


J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

Thank you for submitting an Abreview for ab616. As you may have noticed, your review has now been published on our website.
Since you obtained poor results using the antibody in an untested species, we would like to follow up on this to see if we can possibly improve the results you are seeing with this antibody.
Based on the information in your review, I have a few more questions:
1) Did you use the antibody to detect human beta Galactosidase ?
2) If not, for which application or species was the discount code given?
In terms of suggestions:
- It might be helpful to compare the resuts obtained with human beta Galactosidase with E.coli beta Galactosidase.
If you decide to follow these suggestions, please let me know if they are helpful.
I wish you good luck with your research.

Thank you for contacting us yesterday.

I am sorry to hear you are experiencing difficulties with one of our products. As discussed over the phone, I'd like to try and help you to improve the results currently obtained. In order to do this, could you please fill out the questionnair I have attached to this email. If you could also include some images of the blots obtained that would be very helpful. I understand that you have already performed a lotof optimisation but if you could just briefly outline what you have tried to optimise that would be very helpful.

I think it would be worthwhile to try and use the beta Galactosidase protein (ab7981) which you have just to check that there is not a problem with the antibody itself thatare contributing to the poor results.

In answer to you questions in regards to how to use this protein as a control. We recommend reconstituting the protein in a minimum concentration of 1 mg/mL in 0.1 M Sodium/potassium phosphate buffer, pH 7.0. This can be performed as follows:

0.1 M Sodium phosphate buffer: Dissolve 1.42 gms sodium phosphate dibasic (MW 141.98) in reagent grade water to a final volume of 100 ml.

0.1 M Potassium phosphate buffer: Prepare by dissolving 1.36 gms potassium phosphate monobasic (MW 136.1) in reagent grade water to a final volume of 100 ml.

0.1 M Sodium/potassium phosphate, pH 7.0: Take 60 ml sodium phosphate buffer 0.1 M and adjust pH to 7.0 with 0.1 M potassium phosphate buffer. Buffer may be stored at 2 - 8°C for 2 - 3 weeks.

The lypolised powder the protein is provided as can be stored at2-8°C but once dissolved it is best to aliquot and freeze stocks at -20C or -80C. We do not have any reconstituted stability data andwould therefore recommend to reconstitute the amount needed for the assay fresh just prior to performing the assay.

I would recommend initially loading 0.1 ug in a well to see how well the antibody performs next to your samples. This value may need to be reduced if the signal observed is too strong compared to your lysates.

I hope this information has been of help. I look forward to receiving your reply.

Vielen Dank für Ihre Anfrage.

Leider bieten wir generell keine kostenlosen Proben zu Testzwecken an. Hier bei Abcam verfahren wir so, dass wir einen kostenlosen Ersatz oder eine Gutschrift verschicken, falls ein Antikörper nicht so funktioniert wie auf unserem Datenblatt beschrieben. Falls der Antikörper in einer bisher nicht getesteten Anwendung oder Spezies verwendet wird, können wir leider keinen Ersatz anbieten, wir möchten Sie jedoch einladen unser Abreview-System zu verwenden.

Das Abreview-System erlaubt es unseren Kunden, Rezensionen unserer Produkte zu veröffendlichen und dafür Punkte zu erhalten. Diese “Abpoints” können gegen Rabatte beim Kauf weiterer Produkte oder Geschenkgutscheine wie z.B. Amazon.com-Gutscheine eingetauscht werden. Sollten Sie sich dazu entscheiden, dieses Produkt zu kaufen, teilen Sie uns bitte Ihre Ergebnisse mittels eines „Abreview“ mit. Sie können die Rezension durch einen Link direkt auf unserem Produktdatenblatt abschicken.

Unter der folgenden URL können Sie mehr über unser Abreview-System zu erfahren:

https://www.abcam.com/abreviews

Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Merci pour ces précisions.

Je vous souhaite une bonne continuation dans vos expérimentations. N'hésitez pas à nous contacter de nouveau si vous avez des questions.