Recombinant Anti-beta glucuronidase (GUSB) antibody [EPR10616] (ab166904)
![Western blot - Anti-beta glucuronidase (GUSB) antibody [EPR10616] (ab166904)](https://www.abcam.com/ps/products/166/ab166904/Images/ab166904-165267-anti-beta-glucuronidase-gusb-antibody-epr10616-western-blot.jpg "Western blot - Anti-beta glucuronidase (GUSB) antibody [EPR10616] (ab166904)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10616] to beta glucuronidase (GUSB)
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-beta glucuronidase (GUSB) antibody [EPR10616]
See all beta glucuronidase (GUSB) primary antibodies -
Description
Rabbit monoclonal [EPR10616] to beta glucuronidase (GUSB) -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal liver lysate, K562, HepG2 and 293T cell lysates, paraffin-embedded Human liver and skin tissue, HepG2 cells, K562 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10616 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab166904 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 78 kDa.
|
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/250.
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| Notes |
|---|
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/5000. Predicted molecular weight: 78 kDa. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/250. |
Target
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Function
Plays an important role in the degradation of dermatan and keratan sulfates. -
Involvement in disease
Defects in GUSB are the cause of mucopolysaccharidosis type 7 (MPS7) [MIM:253220]; also known as Sly syndrome. MPS7 is an autosomal recessive lysosomal storage disease characterized by inability to degrade glucuronic acid-containing glycosaminoglycans. The phenotype is highly variable, ranging from severe lethal hydrops fetalis to mild forms with survival into adulthood. Most patients with the intermediate phenotype show hepatomegaly, skeletal anomalies, coarse facies, and variable degrees of mental impairment.
Note=Mucopolysaccharidosis type 7 is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders. -
Sequence similarities
Belongs to the glycosyl hydrolase 2 family. -
Post-translational
modificationsN-linked glycosylated with 3 to 4 oligosaccharide chains. -
Cellular localization
Lysosome. - Information by UniProt
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Database links
- Entrez Gene: 2990 Human
- Omim: 611499 Human
- SwissProt: P08236 Human
- Unigene: 255230 Human
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Alternative names
- Ac2-223 antibody
- asd antibody
- Beta G1 antibody
see all
Images
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Western blot - Anti-beta glucuronidase (GUSB) antibody [EPR10616] (ab166904)All lanes : Anti-beta glucuronidase (GUSB) antibody [EPR10616] (ab166904) at 1/1000 dilution
Lane 1 : Human fetal liver tissue lysate
Lane 2 : K562 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP IgG antibody at 1/2000 dilution
Predicted band size: 78 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta glucuronidase (GUSB) antibody [EPR10616] (ab166904)](https://www.abcam.com/ps/products/166/ab166904/Images/ab166904-165266-anti-beta-glucuronidase-gusb-antibody-epr10616-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta glucuronidase (GUSB) antibody [EPR10616] (ab166904)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling beta glucuronidase (GUSB) with ab166904 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta glucuronidase (GUSB) antibody [EPR10616] (ab166904)](https://www.abcam.com/ps/products/166/ab166904/Images/ab166904-165265-anti-beta-glucuronidase-gusb-antibody-epr10616-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta glucuronidase (GUSB) antibody [EPR10616] (ab166904)Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling beta glucuronidase (GUSB) with ab166904 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-beta glucuronidase (GUSB) antibody [EPR10616] (ab166904)](https://www.abcam.com/ps/products/166/ab166904/Images/ab166904-165264-anti-beta-glucuronidase-gusb-antibody-epr10616-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-beta glucuronidase (GUSB) antibody [EPR10616] (ab166904)Immunofluorescent analysis of HepG2 cells labeling beta glucuronidase (GUSB) with ab166904 at 1/100 dilution. -
![Flow Cytometry (Intracellular) - Anti-beta glucuronidase (GUSB) antibody [EPR10616] (ab166904)](https://www.abcam.com/ps/products/166/ab166904/Images/ab166904-165263-anti-beta-glucuronidase-gusb-antibody-epr10616-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-beta glucuronidase (GUSB) antibody [EPR10616] (ab166904)Intracellular flow cytometric analysis of permeabilized K562 cells labeling beta glucuronidase (GUSB) with ab166904 at 1/100 dilution (red) or a rabbit IgG negative (green).
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![Anti-beta glucuronidase (GUSB) antibody [EPR10616] (ab166904)](https://www.abcam.com/ps/products/166/ab166904/Images/ab166904-6-benefits-of-recombinant-antibodies.png)
Anti-beta glucuronidase (GUSB) antibody [EPR10616] (ab166904)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab166904 has not yet been referenced specifically in any publications.