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RecombinantRabMAb

Recombinant Anti-beta glucuronidase (GUSB) antibody [EPR10616] - BSA and Azide free (ab249385)

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Western blot - Anti-beta glucuronidase (GUSB) antibody [EPR10616] - BSA and Azide free (ab249385)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta glucuronidase (GUSB) antibody [EPR10616] - BSA and Azide free (ab249385)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta glucuronidase (GUSB) antibody [EPR10616] - BSA and Azide free (ab249385)
  • Immunocytochemistry/ Immunofluorescence - Anti-beta glucuronidase (GUSB) antibody [EPR10616] - BSA and Azide free (ab249385)
  • Flow Cytometry (Intracellular) - Anti-beta glucuronidase (GUSB) antibody [EPR10616] - BSA and Azide free (ab249385)
  • Anti-beta glucuronidase (GUSB) antibody [EPR10616] - BSA and Azide free (ab249385)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10616] to beta glucuronidase (GUSB) - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-beta glucuronidase (GUSB) antibody [EPR10616] - BSA and Azide free
    See all beta glucuronidase (GUSB) primary antibodies

  • Description

    Rabbit monoclonal [EPR10616] to beta glucuronidase (GUSB) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab249385 is the carrier-free version of ab166904.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab249385 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 78 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 78 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
Application notes
Is unsuitable for IP.

Target

  • Function

    Plays an important role in the degradation of dermatan and keratan sulfates.

  • Involvement in disease

    Defects in GUSB are the cause of mucopolysaccharidosis type 7 (MPS7) [MIM:253220]; also known as Sly syndrome. MPS7 is an autosomal recessive lysosomal storage disease characterized by inability to degrade glucuronic acid-containing glycosaminoglycans. The phenotype is highly variable, ranging from severe lethal hydrops fetalis to mild forms with survival into adulthood. Most patients with the intermediate phenotype show hepatomegaly, skeletal anomalies, coarse facies, and variable degrees of mental impairment.
    Note=Mucopolysaccharidosis type 7 is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders.

  • Sequence similarities

    Belongs to the glycosyl hydrolase 2 family.

  • Post-translational
    modifications

    N-linked glycosylated with 3 to 4 oligosaccharide chains.

  • Cellular localization

    Lysosome.
  • Information by UniProt

  • Database links

    • Alternative names

      • Ac2-223 antibody
      • asd antibody
      • Beta G1 antibody
      • Beta glucuronidase antibody
      • Beta-G1 antibody
      • Beta-glucuronidase antibody
      • BG antibody
      • BGLR antibody
      • BGLR_HUMAN antibody
      • FLJ39445 antibody
      • Glucuronidase beta antibody
      • Gur antibody
      • Gus antibody
      • Gus-r antibody
      • Gus-s antibody
      • Gus-t antibody
      • Gus-u antibody
      • GUSB antibody
      • Gut antibody
      • MPS7 antibody
      see all

    Images

    • Western blot - Anti-beta glucuronidase (GUSB) antibody [EPR10616] - BSA and Azide free (ab249385)
      Western blot - Anti-beta glucuronidase (GUSB) antibody [EPR10616] - BSA and Azide free (ab249385)
      All lanes : Anti-beta glucuronidase (GUSB) antibody [EPR10616] (ab166904) at 1/1000 dilution

      Lane 1 : Human fetal liver tissue lysate
      Lane 2 : K562 cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP IgG antibody at 1/2000 dilution

      Predicted band size: 78 kDa



      This data was developed using ab166904, the same antibody clone in a different buffer formulation.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta glucuronidase (GUSB) antibody [EPR10616] - BSA and Azide free (ab249385)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta glucuronidase (GUSB) antibody [EPR10616] - BSA and Azide free (ab249385)
      This data was developed using ab166904, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling beta glucuronidase (GUSB) with ab166904 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta glucuronidase (GUSB) antibody [EPR10616] - BSA and Azide free (ab249385)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta glucuronidase (GUSB) antibody [EPR10616] - BSA and Azide free (ab249385)
      This data was developed using ab166904, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling beta glucuronidase (GUSB) with ab166904 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    • Immunocytochemistry/ Immunofluorescence - Anti-beta glucuronidase (GUSB) antibody [EPR10616] - BSA and Azide free (ab249385)
      Immunocytochemistry/ Immunofluorescence - Anti-beta glucuronidase (GUSB) antibody [EPR10616] - BSA and Azide free (ab249385)
      This data was developed using ab166904, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HepG2 cells labeling beta glucuronidase (GUSB) with ab166904 at 1/100 dilution.
    • Flow Cytometry (Intracellular) - Anti-beta glucuronidase (GUSB) antibody [EPR10616] - BSA and Azide free (ab249385)
      Flow Cytometry (Intracellular) - Anti-beta glucuronidase (GUSB) antibody [EPR10616] - BSA and Azide free (ab249385)

      This data was developed using ab166904, the same antibody clone in a different buffer formulation. 

      Intracellular flow cytometric analysis of permeabilized K562 cells labeling beta glucuronidase (GUSB) with ab166904 at 1/100 dilution (red) or a rabbit IgG negative (green).

       

    • Anti-beta glucuronidase (GUSB) antibody [EPR10616] - BSA and Azide free (ab249385)
      Anti-beta glucuronidase (GUSB) antibody [EPR10616] - BSA and Azide free (ab249385)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab249385? Please let us know so that we can cite the reference in this datasheet.

    ab249385 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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