Recombinant Anti-beta I Tubulin antibody [EPR16778] (ab179511)

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Western blot - Anti-beta I Tubulin antibody [EPR16778] (ab179511)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16778] to beta I Tubulin
Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB
Reacts with: Mouse, Rat, Chicken, Hamster, Cow, Dog, Human, African green monkey

Alexa Fluor® 488 APC Carrier Free HRP PE

Product name

Anti-beta I Tubulin antibody [EPR16778]
See all beta I Tubulin primary antibodies
Description

Rabbit monoclonal [EPR16778] to beta I Tubulin
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WBmore details
Species reactivity

Reacts with: Mouse, Rat, Chicken, Hamster, Cow, Dog, Human, African green monkey
Predicted to work with: Monkey
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal brain, fetal heart and fetal kidney lysates; Mouse brain, Rat brain and Rat spleen lysates; K562, Jurkat, HeLa, 293T, C6, RAW 264.7, PC-12, NIH/3T3, UMNSAH/DF-1, BHK, MDCK, MDBK, and COS-1 whole cell lysates. IHC-P: Human tonsil tissue; Mouse cerebral cortex tissue; Rat spleen tissue. ICC/IF: K562 cells. Flow Cyt (intra): K562 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16778
Isotype

IgG
Research areas

Alternative Versions
- Alexa Fluor® 488 Anti-beta I Tubulin antibody [EPR16778] (ab201831)
- Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human beta I Tubulin protein (ab164310)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab179511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt (Intra)		1/80. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF		1/500.
IHC-P		1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB		1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

Notes
Flow Cyt (Intra) 1/80. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/500.
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

Function

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Involvement in disease

Defects in TUBB1 are a cause of macrothrombocytopenia autosomal dominant TUBB1-related (MAD-TUBB1) [MIM:613112]. It is a congenital blood disorder characterized by increased platelet size and decreased number of circulating platelets.
Sequence similarities

Belongs to the tubulin family.
Post-translational
modifications

Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Cellular localization

Cytoplasm > cytoskeleton.
Target information above from: UniProt accession Q9H4B7 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 103243432 African green monkey
- Entrez Gene: 396427 Chicken
- Entrez Gene: 541271 Cow
- Entrez Gene: 485950 Dog
- Entrez Gene: 101836899 Hamster
- Entrez Gene: 81027 Human
- Entrez Gene: 545486 Mouse
- Entrez Gene: 679312 Rat
- Omim: 612901 Human
- SwissProt: P09203 Chicken
- SwissProt: Q9H4B7 Human
- SwissProt: A2AQ07 Mouse
- Unigene: 303023 Human
see all
Alternative names
- 2810484G07Rik antibody
- beta 1 tubulin antibody
- Beta tubulin 1, class VI antibody
- Beta-tubulin antibody
- Class VI beta tubulin antibody
- dJ543J19.4 antibody
- M(beta)1 antibody
- TBB1_HUMAN antibody
- TUBB1 antibody
- Tubulin beta 1 class VI antibody
- Tubulin beta-1 chain antibody
- Tubulin, beta 1 antibody
- tubulin, beta1 antibody
see all

Western blot - Anti-beta I Tubulin antibody [EPR16778] (ab179511)

All lanes : Anti-beta I Tubulin antibody [EPR16778] (ab179511) at 1/20000 dilution

Lane 1 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysates
Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates
Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
Lane 4 : 293T (Human epithelial cells from embryonic kidney) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 50 kDa
Observed band size: 50 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-beta I Tubulin antibody [EPR16778] (ab179511)

All lanes : Anti-beta I Tubulin antibody [EPR16778] (ab179511) at 1/2000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 50 kDa
Observed band size: 50 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-beta I Tubulin antibody [EPR16778] (ab179511)

All lanes : Anti-beta I Tubulin antibody [EPR16778] (ab179511) at 1/2000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : Rat spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 50 kDa
Observed band size: 50 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-beta I Tubulin antibody [EPR16778] (ab179511)

All lanes : Anti-beta I Tubulin antibody [EPR16778] (ab179511) at 1/2000 dilution

Lane 1 : C6 (Rat glial tumor cells) whole cell lysates
Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
Lane 4 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 50 kDa
Observed band size: 50 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-beta I Tubulin antibody [EPR16778] (ab179511)

All lanes : Anti-beta I Tubulin antibody [EPR16778] (ab179511) at 1/1000 dilution

Lane 1 : UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) whole cell lysates
Lane 2 : BHK (Hamster kidney fibroblast cells) whole cell lysates
Lane 3 : MDCK (Canine kidney cell line) whole cell lysates
Lane 4 : MDBK (Bovine kidney cell line) whole cell lysates
Lane 5 : COS-1 (African green monkey kidney fibroblast-like cell line) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 50 kDa
Observed band size: 50 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-beta I Tubulin antibody [EPR16778] (ab179511)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling beta I Tubulin with ab179511 at 1/500 dilution followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/400 dilution (green). Cytoplasm and nuclear staining on K562 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
1. ab179511 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta I Tubulin antibody [EPR16778] (ab179511)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling beta I Tubulin with ab179511 at 1/500 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm staining on germinal center lymphocytes of Human tonsil is observed. Counter stained with Hematoxylin.

Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta I Tubulin antibody [EPR16778] (ab179511)

Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling beta I Tubulin with ab179511 at 1/500 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm staining on neuron cells of Mouse cerebral cortex tissue is observed. Counter stained with Hematoxylin.

Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta I Tubulin antibody [EPR16778] (ab179511)

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling beta I Tubulin with ab179511 at 1/500 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm staining on follicular center lymphocytes of Rat spleen tissue is observed. Counter stained with Hematoxylin.

Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-beta I Tubulin antibody [EPR16778] (ab179511)

Intracellular flow cytometric analysis of2% paraformaldehyde-fixed K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling beta I Tubulin with ab179511 at 1/80 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
Anti-beta I Tubulin antibody [EPR16778] (ab179511)

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

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Datasheet download

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Publishing research using ab179511? Please let us know so that we can cite the reference in this datasheet.

ab179511 has been referenced in 5 publications.

Guo S et al. TRIB2 modulates proteasome function to reduce ubiquitin stability and protect liver cancer cells against oxidative stress. Cell Death Dis 12:42 (2021). PubMed: 33414446
Lin Z et al. Epidermal stem cells maintain stemness via a biomimetic micro/nanofiber scaffold that promotes wound healing by activating the Notch signaling pathway. Stem Cell Res Ther 12:341 (2021). PubMed: 34112252
Ren X et al. PDGF-BB regulates the transformation of fibroblasts into cancer-associated fibroblasts via the lncRNA LURAP1L-AS1/LURAP1L/IKK/I?B/NF-?B signaling pathway. Oncol Lett 22:537 (2021). PubMed: 34079593
Ren L et al. Humanin analogue, HNG, inhibits platelet activation and thrombus formation by stabilizing platelet microtubules. J Cell Mol Med 24:4773-4783 (2020). PubMed: 32174022
Mendelson A et al. Mesenchymal stromal cells lower platelet activation and assist in platelet formation in vitro. JCI Insight 4:N/A (2019). PubMed: 31434805

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Involvement in disease

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (5)

Customer reviews and Q&As

Post-translational
modifications