Anti-beta II Tubulin antibody (ab151318)
Key features and details
- Rabbit polyclonal to beta II Tubulin
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-beta II Tubulin antibody
See all beta II Tubulin primary antibodies -
Description
Rabbit polyclonal to beta II Tubulin -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Monkey, Zebrafish, Xenopus tropicalis
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Immunogen
A recombinant protein fragment corresponding to a region within amino acids 243 and 445 of Human beta II Tubulin (UniProt ID: P68371).
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Positive control
- A431; NIH 3T3; Jurkat; 293T and Raji cell lysates; A431 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151318 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/100 - 1/1000.
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| WB |
1/5000 - 1/20000. Predicted molecular weight: 50 kDa.
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| ICC/IF |
1/100 - 1/1000.
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| Notes |
|---|
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IHC-P
1/100 - 1/1000. |
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WB
1/5000 - 1/20000. Predicted molecular weight: 50 kDa. |
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ICC/IF
1/100 - 1/1000. |
Target
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Function
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the tubulin family. -
Domain
The highly acidic C-terminal region may bind cations such as calcium. -
Post-translational
modificationsSome glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 10383 Human
- Entrez Gene: 347733 Human
- Entrez Gene: 7280 Human
- Entrez Gene: 22151 Mouse
- Entrez Gene: 227613 Mouse
- Entrez Gene: 73710 Mouse
- Entrez Gene: 291081 Rat
- Entrez Gene: 296554 Rat
see all -
Alternative names
- bA506K6.1 antibody
- beta 2 tubulin antibody
- Beta2 antibody
see all
Images
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Western blot - Anti-beta II Tubulin antibody (ab151318)Anti-beta II Tubulin antibody (ab151318) at 1/10000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 50 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta II Tubulin antibody (ab151318)Paraffin-embedded human breast cancer tissue stained beta II tubuin using ab151318 at 1/250 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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Immunocytochemistry/ Immunofluorescence - Anti-beta II Tubulin antibody (ab151318)Immunofluorescence analysis of 4% paraformaldehyde fixed (at RT for 15 min) HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling beta II tubulin at cytoskeleton (green) using ab151318 at 1/500 dilution. Blue: Fluoroshield with DAPI. Scale bar= 10 μm.
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Western blot - Anti-beta II Tubulin antibody (ab151318)All lanes : Anti-beta II Tubulin antibody (ab151318) at 1/10000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 50 kDa10% SDS-PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (19)
ab151318 has been referenced in 19 publications.
- Yen JH et al. Activation of dynamin-related protein 1 - dependent mitochondria fragmentation and suppression of osteosarcoma by cryptotanshinone. J Exp Clin Cancer Res 38:42 (2019). PubMed: 30691497
- Xiao D et al. Licorisoflavan A Exerts Antidepressant-Like Effect in Mice: Involvement of BDNF-TrkB Pathway and AMPA Receptors. Neurochem Res 44:2044-2056 (2019). PubMed: 31278631
- Li EY et al. LncRNA MIAT overexpression reduced neuron apoptosis in a neonatal rat model of hypoxic-ischemic injury through miR-211/GDNF. Cell Cycle 18:156-166 (2019). PubMed: 30563429
- Liu B et al. MiR-361-5p inhibits cell proliferation and induces cell apoptosis in retinoblastoma by negatively regulating CLDN8. Childs Nerv Syst 35:1303-1311 (2019). PubMed: 31161266
- Jiang F et al. Potential Roles of miR-374a-5p in Mediating Neuroprotective Effects and Related Molecular Mechanism. J Mol Neurosci 69:123-132 (2019). PubMed: 31127537