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RecombinantRabMAb

Recombinant Anti-beta IV Tubulin antibody [EPR16775] - BSA and Azide free (ab250103)

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Western blot - Anti-beta IV Tubulin antibody [EPR16775] - BSA and Azide free (ab250103)
  • Western blot - Anti-beta IV Tubulin antibody [EPR16775] - BSA and Azide free (ab250103)
  • Western blot - Anti-beta IV Tubulin antibody [EPR16775] - BSA and Azide free (ab250103)
  • Immunocytochemistry/ Immunofluorescence - Anti-beta IV Tubulin antibody [EPR16775] - BSA and Azide free (ab250103)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16775] - BSA and Azide free (ab250103)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16775] - BSA and Azide free (ab250103)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16775] - BSA and Azide free (ab250103)
  • Flow Cytometry (Intracellular) - Anti-beta IV Tubulin antibody [EPR16775] - BSA and Azide free (ab250103)
  • Anti-beta IV Tubulin antibody [EPR16775] - BSA and Azide free (ab250103)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16775] to beta IV Tubulin - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alkaline Phosphatase APC HRP PE Unconjugated

Overview

  • Product name

    Anti-beta IV Tubulin antibody [EPR16775] - BSA and Azide free
    See all beta IV Tubulin primary antibodies

  • Description

    Rabbit monoclonal [EPR16775] to beta IV Tubulin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250103 is the carrier-free version of ab179504.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab250103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

  • Sequence similarities

    Belongs to the tubulin family.

  • Domain

    The highly acidic C-terminal region may bind cations such as calcium.

  • Post-translational
    modifications

    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.

  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt

  • Database links

    • Alternative names

      • Beta 4 antibody
      • Beta 4 tubulin antibody
      • beta 5 antibody
      • beta four tubulin antibody
      • Dystonia 4 torsion (autosomal dominant) antibody
      • MC1R antibody
      • TBB4_HUMAN antibody
      • TUB B4 antibody
      • TUBB 4 antibody
      • tubb4 antibody
      • TUBB4A antibody
      • TUBB5 antibody
      • Tubulin 5 beta antibody
      • Tubulin beta 3 antibody
      • Tubulin beta 4 antibody
      • Tubulin beta 4 chain antibody
      • Tubulin beta 4A class IVa antibody
      • Tubulin beta 5 antibody
      • Tubulin beta IV antibody
      • Tubulin beta-4 chain antibody
      see all

    Images

    • Western blot - Anti-beta IV Tubulin antibody [EPR16775] - BSA and Azide free (ab250103)
      Western blot - Anti-beta IV Tubulin antibody [EPR16775] - BSA and Azide free (ab250103)
      All lanes : Anti-beta IV Tubulin antibody [EPR16775] (ab179504) at 1/5000 dilution

      Lane 1 : Human fetal brain lysates
      Lane 2 : Human cerebellum lysates
      Lane 3 : Human fetal kidney lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

      Predicted band size: 50 kDa
      Observed band size: 50 kDa



      This data was developed using ab179504, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-beta IV Tubulin antibody [EPR16775] - BSA and Azide free (ab250103)
      Western blot - Anti-beta IV Tubulin antibody [EPR16775] - BSA and Azide free (ab250103)
      All lanes : Anti-beta IV Tubulin antibody [EPR16775] (ab179504) at 1/5000 dilution

      Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
      Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates
      Lane 3 : Neuro-2a (Mouse neuroblastoma cells) whole cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

      Predicted band size: 50 kDa
      Observed band size: 50 kDa



      This data was developed using ab179504, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-beta IV Tubulin antibody [EPR16775] - BSA and Azide free (ab250103)
      Western blot - Anti-beta IV Tubulin antibody [EPR16775] - BSA and Azide free (ab250103)
      All lanes : Anti-beta IV Tubulin antibody [EPR16775] (ab179504) at 1/5000 dilution

      Lane 1 : Mouse brain lysates
      Lane 2 : Mouse heart lysates
      Lane 3 : Mouse kidney lysates
      Lane 4 : Mouse spleen lysates
      Lane 5 : Rat brain lysates
      Lane 6 : Rat heart lysates
      Lane 7 : Rat kidney lysates
      Lane 8 : Rat spleen lysates
      Lane 9 : C6 (Rat glial tumor cells) whole cell lysates
      Lane 10 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
      Lane 11 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
      Lane 12 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

      Predicted band size: 50 kDa
      Observed band size: 50 kDa



      This data was developed using ab179504, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-beta IV Tubulin antibody [EPR16775] - BSA and Azide free (ab250103)
      Immunocytochemistry/ Immunofluorescence - Anti-beta IV Tubulin antibody [EPR16775] - BSA and Azide free (ab250103)
      This data was developed using ab179504, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling beta IV Tubulin with ab179504 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody (ab150077) at 1/200 dilution (green). Cytoplasm staining is observed.Tubulin is detected with Mouse anti-Tubulin antibody (ab7291) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/400 dilution (red). The nuclear counter stain is DAPI (blue). The negative controls:--ve control 1 - ab179504 at 1/100 dilution followed by Goat anti mouse IgG (Alexa Fluor®594) at 1/400 dilution.
      -ve control 2 - Mouse anti-Tubulin antibody (ab7291) at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 dilution.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16775] - BSA and Azide free (ab250103)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16775] - BSA and Azide free (ab250103)
      This data was developed using ab179504, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cerebral cortex issue labeling beta IV Tubulin with ab179504 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Cytoplasm staining is observed on neurons of cerebral cortex. Negative control used PBS instead of ab179504, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16775] - BSA and Azide free (ab250103)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16775] - BSA and Azide free (ab250103)
      This data was developed using ab179504, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Mouse hippocampus tissue labeling beta IV Tubulin with ab179504 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Cytoplasm staining is observed on neurons of mouse hippocampus. Negative control used PBS instead of ab179504, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16775] - BSA and Azide free (ab250103)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16775] - BSA and Azide free (ab250103)
      This data was developed using ab179504, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat thalamus tissue labeling beta IV Tubulin with ab179504 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Cytoplasm staining is observed on neurons of rat thalamus. Negative control used PBS instead of ab179504, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    • Flow Cytometry (Intracellular) - Anti-beta IV Tubulin antibody [EPR16775] - BSA and Azide free (ab250103)
      Flow Cytometry (Intracellular) - Anti-beta IV Tubulin antibody [EPR16775] - BSA and Azide free (ab250103)

      This data was developed using ab179504, the same antibody clone in a different buffer formulation. 

      Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling beta IV Tubulin with ab179504 at 1/20 dilution (red line). Secondary antibody used is a goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is rabbit monoclonal IgG (black line). 

       

       

    • Anti-beta IV Tubulin antibody [EPR16775] - BSA and Azide free (ab250103)
      Anti-beta IV Tubulin antibody [EPR16775] - BSA and Azide free (ab250103)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab250103? Please let us know so that we can cite the reference in this datasheet.

    ab250103 has not yet been referenced specifically in any publications.

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