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RecombinantRabMAb

Recombinant Anti-beta IV Tubulin antibody [EPR16776] (ab179509)

Reviews (1) Submit a question References (5)
Immunocytochemistry/ Immunofluorescence - Anti-beta IV Tubulin antibody [EPR16776] (ab179509)
  • Western blot - Anti-beta IV Tubulin antibody [EPR16776] (ab179509)
  • Western blot - Anti-beta IV Tubulin antibody [EPR16776] (ab179509)
  • Western blot - Anti-beta IV Tubulin antibody [EPR16776] (ab179509)
  • Immunocytochemistry/ Immunofluorescence - Anti-beta IV Tubulin antibody [EPR16776] (ab179509)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16776] (ab179509)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16776] (ab179509)
  • Flow Cytometry (Intracellular) - Anti-beta IV Tubulin antibody [EPR16776] (ab179509)
  • Flow Cytometry (Intracellular) - Anti-beta IV Tubulin antibody [EPR16776] (ab179509)
  • Anti-beta IV Tubulin antibody [EPR16776] (ab179509)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16776] to beta IV Tubulin
  • Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free PE

Overview

  • Product name

    Anti-beta IV Tubulin antibody [EPR16776]
    See all beta IV Tubulin primary antibodies

  • Description

    Rabbit monoclonal [EPR16776] to beta IV Tubulin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal liver lysate, Human fetal heart lysate, Human fetal kidney lysate, Human cerebellum lysate, HeLa whole cell lysate, Jurkat whole cell lysate, Neuro-2a whole cell lysate, Mouse brain lysate, Mouse spleen lysate , Rat brain lysate, Rat spleen lysate, C6 whole cell lysate, Raw264.7 whole cell lysate, PC-12 whole cell lysate, NIH/3T3 whole cell lysate. IHCP: Mouse cerebrum and Mouse testis tissues.IF/ICC and Flow Cyt (intra): HeLa and C6 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab179509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/150.
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC application is recommended for mouse only.
WB
1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
ICC/IF (1)
1/500.
Notes
Flow Cyt (Intra)
1/150.
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC application is recommended for mouse only.
WB
1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
ICC/IF
1/500.

Target

  • Function

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

  • Sequence similarities

    Belongs to the tubulin family.

  • Domain

    The highly acidic C-terminal region may bind cations such as calcium.

  • Post-translational
    modifications

    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.

  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt

  • Database links

    • Alternative names

      • Beta 4 antibody
      • Beta 4 tubulin antibody
      • beta 5 antibody
      • beta four tubulin antibody
      • Dystonia 4 torsion (autosomal dominant) antibody
      • MC1R antibody
      • TBB4_HUMAN antibody
      • TUB B4 antibody
      • TUBB 4 antibody
      • tubb4 antibody
      • TUBB4A antibody
      • TUBB5 antibody
      • Tubulin 5 beta antibody
      • Tubulin beta 3 antibody
      • Tubulin beta 4 antibody
      • Tubulin beta 4 chain antibody
      • Tubulin beta 4A class IVa antibody
      • Tubulin beta 5 antibody
      • Tubulin beta IV antibody
      • Tubulin beta-4 chain antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-beta IV Tubulin antibody [EPR16776] (ab179509)
      Immunocytochemistry/ Immunofluorescence - Anti-beta IV Tubulin antibody [EPR16776] (ab179509)

      Immunofluorescent analysis of 100% methanol fixed C6 (Rat glial tumor cell line ) labeling beta IV Tubulin with ab179509 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution(green).

      Cytoplasm staining on C6 cell line is observed.

      The nuclear counterstain is DAPI (blue).

      Tubulin is detected with Anti-alpha Tubulin antibody [EPR16776] -Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).
      The negative controls are as follows:-
      -ve control 1 : ab179509 at 1/500 dilution followed by ab150120 at 1/1000 dilution.
      -ve control 2.: ab7291  at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

    • Western blot - Anti-beta IV Tubulin antibody [EPR16776] (ab179509)
      Western blot - Anti-beta IV Tubulin antibody [EPR16776] (ab179509)
      All lanes : Anti-beta IV Tubulin antibody [EPR16776] (ab179509) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : Human fetal heart lysate
      Lane 3 : Human fetal kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

      Developed using the ECL technique.

      Predicted band size: 50 kDa
      Observed band size: 50 kDa


      Exposure time: 30 seconds


      Blocking and diluting buffer was 5% NFDM /TBST

    • Western blot - Anti-beta IV Tubulin antibody [EPR16776] (ab179509)
      Western blot - Anti-beta IV Tubulin antibody [EPR16776] (ab179509)
      All lanes : Anti-beta IV Tubulin antibody [EPR16776] (ab179509) at 1/1000 dilution

      Lane 1 : Human cerebellum lysate
      Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
      Lane 4 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

      Developed using the ECL technique.

      Predicted band size: 50 kDa
      Observed band size: 50 kDa


      Exposure time: 30 seconds


      Blocking and diluting buffer was 5% NFDM /TBST.

      Exposure time - Lane 1: 1 second; Lanes 2,3 and 4: 30 seconds

    • Western blot - Anti-beta IV Tubulin antibody [EPR16776] (ab179509)
      Western blot - Anti-beta IV Tubulin antibody [EPR16776] (ab179509)
      All lanes : Anti-beta IV Tubulin antibody [EPR16776] (ab179509) at 1/1000 dilution

      Lane 1 : Mouse brain lysate
      Lane 2 : Mouse spleen lysate
      Lane 3 : Rat brain lysate
      Lane 4 : Rat spleen lysate
      Lane 5 : C6 (Rat glial tumor cell line) whole cell lysate
      Lane 6 : Raw264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
      Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
      Lane 8 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Predicted band size: 50 kDa
      Observed band size: 50 kDa


      Exposure time: 30 seconds


      Blocking and diluting buffer was 5% NFDM /TBST.
      Exposure time - Lane 1: 1 second; Lane 2: 30 seconds; Lanes 3 and 4: 3 minutes; Lanes 5, 6, 7 and 8: 30 seconds

    • Immunocytochemistry/ Immunofluorescence - Anti-beta IV Tubulin antibody [EPR16776] (ab179509)
      Immunocytochemistry/ Immunofluorescence - Anti-beta IV Tubulin antibody [EPR16776] (ab179509)

      Immunofluorescent analysis of 100% methanol fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cell line labeling beta IV Tubulin with ab179509 at 1/500 dilution, followed by Goat Anti-Rabbit IgG  (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Cytoplasm staining on HeLa cell line is observed.

      The nuclear counterstain is DAPI (blue).

      Tubulin is detected with Anti-alpha Tubulin antibody [EPR16776] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L  (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).

      The negative controls are as follows:-
      -ve control 1 - ab179509 at 1/500  followed by ab150120 at 1/1000.
      -ve control 2. - ab7291 at 1/1000 followed by ab150077  at 1/1000.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16776] (ab179509)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16776] (ab179509)

      Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling beta IV Tubulin with ab179509 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Mouse cerebrum is observed. Counter stained with Hematoxylin.

      Negative control: Used PBS instead of primary antibody,secondary antibody is ab97051 at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16776] (ab179509)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16776] (ab179509)

      Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling beta IV Tubulin with ab179509 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Mouse testis is observed. Counter stained with Hematoxylin.
      Negative control: Used PBS instead of primary antibody,secondary antibody is ab97051 at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-beta IV Tubulin antibody [EPR16776] (ab179509)
      Flow Cytometry (Intracellular) - Anti-beta IV Tubulin antibody [EPR16776] (ab179509)

      Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell linefrom cervix adenocarcinoma) cell linelabeling beta IV Tubulinwith ab179509 at 1/150 dilution (red) compared with a Rabbit IgG,monoclonal [EPR16776]-Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/500 dilutionwas used as the secondary antibody. 

       

       

    • Flow Cytometry (Intracellular) - Anti-beta IV Tubulin antibody [EPR16776] (ab179509)
      Flow Cytometry (Intracellular) - Anti-beta IV Tubulin antibody [EPR16776] (ab179509)

      Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed C6 (Rat glial tumor cell line) labeling beta IV Tubulin with ab179509 at 1/150 (red) compared with a Rabbit IgG,monoclonal [EPR16776]-Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/500 dilutionwas used as the secondary antibody. 

       

       

    • Anti-beta IV Tubulin antibody [EPR16776] (ab179509)
      Anti-beta IV Tubulin antibody [EPR16776] (ab179509)

    References (5)

    Publishing research using ab179509? Please let us know so that we can cite the reference in this datasheet.

    ab179509 has been referenced in 5 publications.

    • Ernst K  et al. Pharmacological targeting of host chaperones protects from pertussis toxin in vitro and in vivo. Sci Rep 11:5429 (2021). PubMed: 33686161
    • Calandria JM  et al. Elovanoids downregulate SARS-CoV-2 cell-entry, canonical mediators and enhance protective signaling in human alveolar cells. Sci Rep 11:12324 (2021). PubMed: 34112906
    • Liu WK  et al. Protein profile of well-differentiated versus un-differentiated human bronchial/tracheal epithelial cells. Heliyon 6:e04243 (2020). PubMed: 32613119
    • Yang J  et al. The Natural Compound Withaferin A Covalently Binds to Cys239 of ß-Tubulin to Promote Tubulin Degradation. Mol Pharmacol 96:711-719 (2019). PubMed: 31585985
    • Child KM  et al. The Neuroregenerative Capacity of Olfactory Stem Cells Is Not Limitless: Implications for Aging. J Neurosci 38:6806-6824 (2018). PubMed: 29934351

    Customer reviews and Q&As

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-beta IV Tubulin antibody [EPR16776]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (human tracheal cultures)
    Permeabilization
    Yes - Saponin
    Specification
    human tracheal cultures
    Blocking step
    Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 18 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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