Recombinant Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901)
![Western blot - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901)](https://www.abcam.com/ps/products/52/ab52901/Images/ab52901-221361-anti-beta-tubulin-antibody-ep1331y-microtubule-marker-western-blot.jpg "Western blot - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1331Y] to beta Tubulin - Microtubule Marker
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker
See all beta Tubulin primary antibodies -
Description
Rabbit monoclonal [EP1331Y] to beta Tubulin - Microtubule Marker -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Zebrafish
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Immunogen
Synthetic peptide within Human beta Tubulin aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P07437 -
Positive control
- WB: PC12 and HeLa whole cell lysate (ab150035) and mouse brain, rat brain, mouse spinal cord and rat spinal cord tissue lysates. IHC-P: Human gastric carcinoma. ICC/IF: HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1331Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52901 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (6) |
1/20000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
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| IHC-P | (2) |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/20000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. -
Tissue specificity
Ubiquitously expressed with highest levels in spleen, thymus and immature brain. -
Involvement in disease
Cortical dysplasia, complex, with other brain malformations 6
Skin creases, congenital symmetric circumferential, 1 -
Sequence similarities
Belongs to the tubulin family. -
Domain
The highly acidic C-terminal region may bind cations such as calcium. -
Post-translational
modificationsSome glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.
Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules. -
Cellular localization
Cytoplasm, cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 203068 Human
- Entrez Gene: 22154 Mouse
- Entrez Gene: 29214 Rat
- Entrez Gene: 386701 Zebrafish
- Omim: 191130 Human
- SwissProt: P07437 Human
- SwissProt: P99024 Mouse
- SwissProt: P69897 Rat
see all -
Alternative names
- Beta 4 tubulin antibody
- Beta 5 tubulin antibody
- beta Ib tubulin antibody
see all
Images
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Western blot - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901)All lanes : Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901) at 1/20000 dilution
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : Spinal Cord (Mouse) Tissue Lysate
Lane 4 : Spinal Cord (Rat) Tissue Lysate
Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution
Predicted band size: 50 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab52901 overnight at 4°C. Antibody binding was detected using ab175781 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
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![Western blot - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901)](https://www.abcam.com/ps/products/52/ab52901/Images/ab52901-65379-anti-beta-tubulin-antibody-ep1331y-microtubule-marker-western-blot.gif)
Western blot - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901)Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901) at 1/20000 dilution + HeLa cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP-conjugated at 1/2000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901)](https://www.abcam.com/ps/products/52/ab52901/Images/ab52901-65378-anti-beta-tubulin-antibody-ep1331y-microtubule-marker-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901)ab52901 at 1/250 dilution staining beta Tubulin in human gastric carcinoma by Immunohistochemsitry, Paraffin embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901)](https://www.abcam.com/ps/products/52/ab52901/Images/ab52901-65376-anti-beta-tubulin-antibody-ep1331y-microtubule-marker-western-blot.jpg)
Western blot - Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901)This image is courtesy of an anonymous AbreviewAnti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901) at 1/1000 dilution (in PBS +0.5% Tween20 for 2 hours at 23°C) + 293 human embryonic kidney whole cell lysate at 25 µg
Secondary
An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 45 seconds
Blocking Step: 5% Milk for 1 hour at 23°C -
![Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901)](https://www.abcam.com/ps/products/52/ab52901/Images/ab52901-8-benefits-of-recombinant-antibodies.png)
Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker (ab52901)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (38)
ab52901 has been referenced in 38 publications.
- Okawa ER et al. Essential roles of insulin and IGF-1 receptors during embryonic lineage development. Mol Metab 47:101164 (2021). PubMed: 33453419
- Eisa A et al. The protein YWHAE (14-3-3 epsilon) in spermatozoa is essential for male fertility. Andrology 9:312-328 (2021). PubMed: 32657535
- Yang T et al. Enolase 1 regulates stem cell-like properties in gastric cancer cells by stimulating glycolysis. Cell Death Dis 11:870 (2020). PubMed: 33067426
- Chang B et al. Nanofibrous Tubular Three-Dimensional Platform for Single Dental Pulp Stem Cell Polarization. ACS Appl Mater Interfaces 12:54481-54488 (2020). PubMed: 33252216
- Van der Perren A et al. The structural differences between patient-derived a-synuclein strains dictate characteristics of Parkinson's disease, multiple system atrophy and dementia with Lewy bodies. Acta Neuropathol 139:977-1000 (2020). PubMed: 32356200