Name: Anti-beta Tubulin antibody [EPR16774]
Brand: RabMAb
SKU: ab179513
Rating: 5 (1 reviews)

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Western blot - Anti-beta Tubulin antibody [EPR16774] (ab179513)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16774] to beta Tubulin
Suitable for: IHC-P, WB, ICC/IF, Flow Cyt (Intra)
Reacts with: Mouse, Rat, Chicken, Cow, Dog, Human, Drosophila melanogaster, Zebrafish, African green monkey, Xenopus tropicalis, Recombinant fragment

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free HRP

Product name

Anti-beta Tubulin antibody [EPR16774]
See all beta Tubulin primary antibodies
Description

Rabbit monoclonal [EPR16774] to beta Tubulin
Host species

Rabbit
Tested applications

Suitable for: IHC-P, WB, ICC/IF, Flow Cyt (Intra)more details
Species reactivity

Reacts with: Mouse, Rat, Chicken, Cow, Dog, Human, Drosophila melanogaster, Zebrafish, African green monkey, Xenopus tropicalis, Recombinant fragment
Predicted to work with: Monkey
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human beta I Tubulin recombinant protein; Zebrafish whole lysate; Xenopus tropicalis lysate; Drosophila whole lysate; UMNSAH/DF-1, MDCK, MDBK, COS-1, HeLa, Jurkat, A431, C6, RAW 264.7, PC-12, and NIH/3T3 whole cell lysates; Human fetal brain and fetal kidney lysates; Mouse brain and Rat brain lysates. IHC-P: Human cerebral cortex, Human kidney, Human glioma, mouse cerebral cortex and rat cerebral cortex tissues. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16774
Isotype

IgG
Research areas
- Neuroscience
- Development

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab179513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-P		1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB		1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
ICC/IF		1/1000.
Flow Cyt (Intra)		1/150. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Notes
IHC-P 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
ICC/IF 1/1000.
Flow Cyt (Intra) 1/150. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Function

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Tissue specificity

Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
Involvement in disease

Cortical dysplasia, complex, with other brain malformations 6
Skin creases, congenital symmetric circumferential, 1
Sequence similarities

Belongs to the tubulin family.
Domain

The highly acidic C-terminal region may bind cations such as calcium.
Post-translational
modifications

Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.
Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
Cellular localization

Cytoplasm, cytoskeleton.
Target information above from: UniProt accession P07437 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 396254 Chicken
- Entrez Gene: 533307 Cow
- Entrez Gene: 203068 Human
- Entrez Gene: 22154 Mouse
- Entrez Gene: 29214 Rat
- Entrez Gene: 386701 Zebrafish
- Omim: 191130 Human
- SwissProt: Q2KJD0 Cow
- SwissProt: P07437 Human
- SwissProt: P99024 Mouse
- SwissProt: P69897 Rat
- Unigene: 636480 Human
see all
Alternative names
- Beta 4 tubulin antibody
- Beta 5 tubulin antibody
- beta Ib tubulin antibody
- Beta1 tubulin antibody
- Class I beta tubulin antibody
- M40 antibody
- MGC117247 antibody
- MGC16435 antibody
- OK/SW cl.56 antibody
- OK/SWcl.56 antibody
- TBB5_HUMAN antibody
- TUBB 1 antibody
- TUBB 2 antibody
- TUBB 5 antibody
- TUBB antibody
- TUBB1 antibody
- TUBB2 antibody
- TUBB5 antibody
- tubulin beta 1 chain antibody
- Tubulin beta 2 chain antibody
- tubulin beta 5 chain antibody
- Tubulin beta chain antibody
- Tubulin beta class I antibody
- tubulin beta polypeptide antibody
- Tubulin beta-5 chain antibody
see all

Western blot - Anti-beta Tubulin antibody [EPR16774] (ab179513)

Anti-beta Tubulin antibody [EPR16774] (ab179513) at 1/1000 dilution + Human beta I Tubulin recombinant protein at 0.01 µg

Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 50 kDa
Observed band size: 50 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

The immunogen of ab179513 has 67% identities with beta I Tubulin. The WB image shows it cross-reacts with beta I Tubulin.
Human beta I Tubulin is an in house recombinant protein (aa1-451) with a proprietary tag.
Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [EPR16774] (ab179513)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling beta Tubulin with ab179513 at 1/1000 dilution, followed by anti-rabbit Alexa Fluor® 488 (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and anti-mouse AlexaFluor® 594 (ab150120) at 1/500 dilution (red).
The negative controls are as follows;
-ve control 1: ab179513 at 1/1000 dilution followed by anti-mouse AlexaFluor® 594 (ab150120) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by anti-rabbit Alexa Fluor® 488 (ab150077) at 1/500 dilution.
Flow Cytometry (Intracellular) - Anti-beta Tubulin antibody [EPR16774] (ab179513)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling beta Tubulin with ab179513 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
Western blot - Anti-beta Tubulin antibody [EPR16774] (ab179513)

All lanes : Anti-beta Tubulin antibody [EPR16774] (ab179513) at 1/2000 dilution

Lane 1 : Zebrafish whole lysates
Lane 2 : Xenopus tropicalis lysates
Lane 3 : Drosophila whole lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 50 kDa
Observed band size: 50 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

The amino acid sequence of ab179513 immunogen is identical to those of tubulin beta 2A, 2B, 3, 4A, 4B, 5, 6 and 8 at the same region.
Western blot - Anti-beta Tubulin antibody [EPR16774] (ab179513)

All lanes : Anti-beta Tubulin antibody [EPR16774] (ab179513) at 1/2000 dilution

Lane 1 : UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) whole cell lysates
Lane 2 : MDCK (Canine kidney cell line) whole cell lysates
Lane 3 : MDBK (Bovine kidney cell line) whole cell lysates
Lane 4 : COS-1 (African green monkey kidney fibroblast-like cell line) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 50 kDa
Observed band size: 50 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

The amino acid sequence of ab179513 immunogen is identical to those of tubulin beta 2A, 2B, 3, 4A, 4B, 5, 6 and 8 at the same region.
Western blot - Anti-beta Tubulin antibody [EPR16774] (ab179513)

All lanes : Anti-beta Tubulin antibody [EPR16774] (ab179513) at 1/2000 dilution

Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates
Lane 3 : A431 (Human epidermoid carcinoma) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 50 kDa
Observed band size: 50 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

The amino acid sequence of ab179513 immunogen is identical to those of tubulin beta 2A, 2B, 3, 4A, 4B, 5, 6 and 8 at the same region.
Western blot - Anti-beta Tubulin antibody [EPR16774] (ab179513)

All lanes : Anti-beta Tubulin antibody [EPR16774] (ab179513) at 1/2000 dilution

Lane 1 : Human fetal brain lysates
Lane 2 : Human fetal kidney lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 50 kDa
Observed band size: 50 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

The amino acid sequence of ab179513 immunogen is identical to those of tubulin beta 2A, 2B, 3, 4A, 4B, 5, 6 and 8 at the same region.
Western blot - Anti-beta Tubulin antibody [EPR16774] (ab179513)

All lanes : Anti-beta Tubulin antibody [EPR16774] (ab179513) at 1/2000 dilution

Lane 1 : Mouse brain lysates
Lane 2 : Rat brain lysates
Lane 3 : C6 (Rat glial tumor cells) whole cell lysates
Lane 4 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
Lane 6 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 50 kDa
Observed band size: 50 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

The amino acid sequence of ab179513 immunogen is identical to those of tubulin beta 2A, 2B, 3, 4A, 4B, 5, 6 and 8 at the same region.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Tubulin antibody [EPR16774] (ab179513)

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling beta Tubulin with ab179513 at 1/250 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasmic staining on neurons of human cerebral cortex is observed. Counter stained with Hematoxylin.

Negative control: Using PBS instead of primary antibody, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Tubulin antibody [EPR16774] (ab179513)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling beta Tubulin with ab179513 at 1/250 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasmic staining on tubules and the glomerulus of human kidney is observed. Counter stained with Hematoxylin.

Negative control: Using PBS instead of primary antibody, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Tubulin antibody [EPR16774] (ab179513)

Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling beta Tubulin with ab179513 at 1/250 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasmic staining on human glioma is observed. Counter stained with Hematoxylin.

Negative control: Using PBS instead of primary antibody, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Tubulin antibody [EPR16774] (ab179513)

Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling beta Tubulin with ab179513 at 1/250 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasm staining on neurons of mouse cerebral cortex is observed. Counter stained with Hematoxylin.

Negative control: Using PBS instead of primary antibody, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Tubulin antibody [EPR16774] (ab179513)

Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling beta Tubulin with ab179513 at 1/250 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasm staining on neurons of rat cerebral cortex is observed. Counter stained with Hematoxylin.

Negative control: Using PBS instead of primary antibody, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-beta Tubulin antibody [EPR16774] (ab179513)

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab179513? Please let us know so that we can cite the reference in this datasheet.

ab179513 has been referenced in 51 publications.

Shannon CE et al. Insulin resistance is mechanistically linked to hepatic mitochondrial remodeling in non-alcoholic fatty liver disease. Mol Metab 45:101154 (2021). PubMed: 33359401
Zhang T et al. Polysaccharides extracted from Rheum tanguticum ameliorate radiation-induced enteritis via activation of Nrf2/HO-1. J Radiat Res 62:46-57 (2021). PubMed: 33140083
Peretti D et al. TrkB signaling regulates the cold-shock protein RBM3-mediated neuroprotection. Life Sci Alliance 4:N/A (2021). PubMed: 33563652
Chen Y et al. Effects of differential distributed-JUP on the malignancy of gastric cancer. J Adv Res 28:195-208 (2021). PubMed: 33364056
Dong H & Song J miR-142-3p reduces the viability of human cervical cancer cells by negatively regulating the cytoplasmic localization of HMGB1. Exp Ther Med 21:212 (2021). PubMed: 33500702

View all Publications for this product

Customer reviews and Q&As

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Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (Testis, adult)

Permeabilization

Yes - 0.1% Triton X-100 in PBS

Specification

Testis, adult

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Bryan Niedenberger

Verified customer

Submitted Aug 04 2016

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Conjugation kits

Isotype control

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Involvement in disease

Sequence similarities

Domain

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (51)

Customer reviews and Q&As

Immunohistochemistry (Frozen sections) abreview for Anti-beta Tubulin antibody [EPR16774]

Post-translational
modifications