For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    products/primary-antibodies/bim-antibody-y36-ab32158.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cell Biology Apoptosis Intracellular Associated Proteins
Share by email
Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Bim antibody [Y36] (ab32158)

  • Datasheet
  • SDS
Reviews (6)Q&A (1)References (78)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-Bim antibody [Y36] (ab32158)
  • Flow Cytometry (Intracellular) - Anti-Bim antibody [Y36] (ab32158)
  • Immunocytochemistry/ Immunofluorescence - Anti-Bim antibody [Y36] (ab32158)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bim antibody [Y36] (ab32158)
  • Flow Cytometry (Intracellular) - Anti-Bim antibody [Y36] (ab32158)
  • Immunoprecipitation - Anti-Bim antibody [Y36] (ab32158)
  • Western blot - Anti-Bim antibody [Y36] (ab32158)
  • Immunocytochemistry/ Immunofluorescence - Anti-Bim antibody [Y36] (ab32158)
  • Flow Cytometry (Intracellular) - Anti-Bim antibody [Y36] (ab32158)
  • Western blot - Anti-Bim antibody [Y36] (ab32158)
  • Western blot - Anti-Bim antibody [Y36] (ab32158)
  • Anti-Bim antibody [Y36] (ab32158)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y36] to Bim
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, IP, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

APC Carrier Free HRP PE

You may also be interested in

Protein
Product image
Recombinant Human Bim protein (ab112387)
Assay
Annexin V/ANXA5-Cy5 Apoptosis Staining / Detection Reagent (ab14147)
Primary
Product image
Anti-Bcl-XL antibody [E18] - BSA and Azide free (ab199099)

View more associated products

Overview

  • Product name

    Anti-Bim antibody [Y36]
    See all Bim primary antibodies
  • Description

    Rabbit monoclonal [Y36] to Bim
  • Host species

    Rabbit
  • Specificity

    The antibody can recognize all isoforms of Bim. The antibody does not cross-react with other Bcl-2 family members.
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Bim aa 1-100. The exact sequence is proprietary.
    (Peptide available as ab179844)

  • Positive control

    • WB : Raji, Jurkat, A431, Molt-4, A20, MEF, Raw264.7 and PC-12 cell lysate; Human and mouse thymus, mouse and rat spleen tissue lysate. IHC : breast carcinoma tissue. ICC/IF : A20 and Raji cells. Flow Cyt (intra) : A431 and Raji whole cell lysate. HAP1-WT cells. IP : Raji whole cell lysate.
  • General notes

    Mouse and Rat species are recommended based on WB results, we do not guarantee IHC-P for Mouse and Rat.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    Y36
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Associated Proteins
    • Cell Biology
    • Apoptosis
    • Intracellular
    • Bcl2 Family
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • MAPK Pathway
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Bcl 2 family
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Cell survival & death
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Bcl 2 family
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Alternative Versions

    • Anti-Bim antibody [Y36] - BSA and Azide free (ab170589)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Bim peptide (ab179844)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human Bim protein (ab112387)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab32158 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/50 - 1/100.
WB (5)
1/500 - 1/2000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).Can be blocked with Bim peptide (ab179844).
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Mouse and Rat species are recommended based on WB results, we do not guarantee IHC-P for Mouse and Rat.

IP (1)
1/40 - 1/50.
ICC/IF
1/100 - 1/250.
Notes
Flow Cyt (Intra)
1/50 - 1/100.
WB
1/500 - 1/2000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).Can be blocked with Bim peptide (ab179844).
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Mouse and Rat species are recommended based on WB results, we do not guarantee IHC-P for Mouse and Rat.

IP
1/40 - 1/50.
ICC/IF
1/100 - 1/250.

Target

  • Function

    Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.
  • Tissue specificity

    Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney.
  • Sequence similarities

    Belongs to the Bcl-2 family.
  • Domain

    The BH3 motif is required for Bcl-2 binding and cytotoxicity.
  • Cellular localization

    Mitochondrion and Endomembrane system. Associated with intracytoplasmic membranes.
  • Target information above from: UniProt accession O43521 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10018 Human
    • Entrez Gene: 12125 Mouse
    • Entrez Gene: 64547 Rat
    • Omim: 603827 Human
    • SwissProt: O43521 Human
    • SwissProt: O54918 Mouse
    • SwissProt: O88498 Rat
    • Unigene: 469658 Human
    • Unigene: 141083 Mouse
    • Unigene: 453214 Mouse
    • Unigene: 82709 Rat
    see all
  • Alternative names

    • BCL2 like 11 antibody
    • B2L11_HUMAN antibody
    • BAM antibody
    • Bcl 2 interacting protein Bim antibody
    • Bcl 2 related ovarian death agonist antibody
    • Bcl-2-like protein 11 antibody
    • BCL2 interacting mediator of cell death antibody
    • BCL2 like 11 (apoptosis facilitator) antibody
    • BCL2 like protein 11 antibody
    • Bcl2-interacting mediator of cell death antibody
    • Bcl2-L-11 antibody
    • Bcl2l11 antibody
    • BIM alpha6 antibody
    • BIM antibody
    • BIM beta6 antibody
    • BIM beta7 antibody
    • BimEL antibody
    • BimL antibody
    • BOD antibody
    see all

Images

  • Western blot - Anti-Bim antibody [Y36] (ab32158)
    Western blot - Anti-Bim antibody [Y36] (ab32158)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: Bim knockout HAP1 whole cell lysate (20 µg)
    Lane 3: Raji whole cell lysate (20 µg)
    Lane 4: Jurkat whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab32158 observed at 25 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab32158 was shown to specifically react with Bim when Bim knockout samples were used. Wild-type and Bim knockout samples were subjected to SDS-PAGE. ab32158 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry (Intracellular) - Anti-Bim antibody [Y36] (ab32158)
    Flow Cytometry (Intracellular) - Anti-Bim antibody [Y36] (ab32158)

    Overlay histogram showing HAP1 wildtype (green line) and HAP1-BCL2L11 knockout cells (red line) stained with ab32158. The cells were fixed 80% methanol (5 min), and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab32158, 1µg/ml) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) presorbed (ab150081) at 1/2000 dilution for 30 min at 22°C. A rabbit IgG1 isotype control antibody (ab172730) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-BCL2L11 knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity). Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. 

     

     

  • Immunocytochemistry/ Immunofluorescence - Anti-Bim antibody [Y36] (ab32158)
    Immunocytochemistry/ Immunofluorescence - Anti-Bim antibody [Y36] (ab32158)

    Immunocytochemistry/Immunofluorescence analysis of Raji (Human Burkitt's lymphoma cell line) labeling Bim with ab32158 at a dilution of 1/250. Cells were fixed with 100% methanol. Ab150077 (1/1000) was used as the secondary antibody. Cells were co-stained with ab7291, a mouse anti-tubulin antibody (1/200) using ab150120 as the secondary. Nuclei were counterstained with DAPI (blue).

    Secondary antibody only control, cells without incubation with the primary antibody was used as negative control.

    Confocal image showing cytoplasmic staining on Raji cell line

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bim antibody [Y36] (ab32158)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bim antibody [Y36] (ab32158)

    ab32158 staining Bim in human breast cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediated antigen retrieval using Tris/EDTA Buffer, PH9 (ab93684). Samples were incubated with primary antibody (1/100 in blocking buffer) and a Biotin-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody. Cytoplasmic staining can be seen in the human breast cancer cells. Hematoxylin was used as a counter stain.

  • Flow Cytometry (Intracellular) - Anti-Bim antibody [Y36] (ab32158)
    Flow Cytometry (Intracellular) - Anti-Bim antibody [Y36] (ab32158)

    Intracellular Flow Cytometry analysis of Raji (human Burkitt's lymphoma) whole cell lysate labeling Bim with ab32158 at 1/100 (red). Cells were fixed with 4% paraformaldehyde. An Alexa Fluorr® 488)-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG (ab172730). Blue - Unlabelled control, cells without incubation with primary and secondary antibodies. 

     

     

  • Immunoprecipitation - Anti-Bim antibody [Y36] (ab32158)
    Immunoprecipitation - Anti-Bim antibody [Y36] (ab32158)

    Ab32158 at 1/50 immunoprecipitating Bim in Raji (human Burkitt's lymphoma) whole cell lysate.

    Lane 1 (input): Raji whole cell lysate (10µg)

    Lane 2 (+): ab32158 + Raji whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32158 in Raji whole cell lysate.

    For western blotting, ab32158 (1/1000) was used as the primary antibody and ab131366 VeriBlot for IP Detection Reagent (HRP) was used for detection (1/10 000).

     

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-Bim antibody [Y36] (ab32158)
    Western blot - Anti-Bim antibody [Y36] (ab32158)
    All lanes : Anti-Bim antibody [Y36] (ab32158) at 1/2000 dilution

    Lane 1 : Raji (human Burkitt's lymphoma) whole cell lysate
    Lane 2 : A431 (human epidermoid carcinoma) whole cell lysate
    Lane 3 : Molt-4 (human acute lymphoblastic leukemia) whole cell lysate
    Lane 4 : Human thymus tissue lysate
    Lane 5 : Mouse thymus tissue lysate
    Lane 6 : A20 (mouse reticulum cell sarcoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 22 kDa



    Observed band size : 22, 18 kDa

    Exposure time : Lane 1- 5: 3 minutes; Lane 6: 2 seconds

    Blocking/Diluting buffer and concentration : 5% NFDM /TBST 

    The observed molecular weight is consistent with the literature (PMID: 24872388)

  • Immunocytochemistry/ Immunofluorescence - Anti-Bim antibody [Y36] (ab32158)
    Immunocytochemistry/ Immunofluorescence - Anti-Bim antibody [Y36] (ab32158)

    Immunocytochemistry/Immunofluorescence analysis of A20 (Mouse reticulum sarcoma cell line) labeling Bim with ab32158 at a dilution of 1/250. Cells were fixed with 100% methanol. Ab150077 (1/1000) was used as the secondary antibody. Cells were co-stained with ab7291, a mouse anti-tubulin antibody (1/200) using ab150120 as the secondary. Nuclei were counterstained with DAPI (blue). 

    Secondary antibody only control, cells without incubation with the primary antibody was used as negative control.

    Confocal image showing cytoplasmic staining on A20 cell line

  • Flow Cytometry (Intracellular) - Anti-Bim antibody [Y36] (ab32158)
    Flow Cytometry (Intracellular) - Anti-Bim antibody [Y36] (ab32158)

    Intracellular Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labelling Bim with ab32158 at 1/50 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. An Alexa Fluorr®488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies. 

     

     

  • Western blot - Anti-Bim antibody [Y36] (ab32158)
    Western blot - Anti-Bim antibody [Y36] (ab32158)
    All lanes : Anti-Bim antibody [Y36] (ab32158) at 1/2000 dilution

    Lane 1 : Mouse spleen tissue lysate
    Lane 2 : Rat spleen tissue lysate
    Lane 3 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 4 : Raw264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 22 kDa



    Observed band size : 22, 18 kDa

    Exposure time : Lane 1-3: 3 minutes; Lane 4: 10 seconds

    Blocking/Diluting buffer and concentration : 5% NFDM /TBST

    The observed molecular weight is consistent with the literature (PMID: 24872388)

  • Western blot - Anti-Bim antibody [Y36] (ab32158)
    Western blot - Anti-Bim antibody [Y36] (ab32158)This image is courtesy of an anonymous abreview.
    Anti-Bim antibody [Y36] (ab32158) at 1/500 dilution + Whole cell lysates prepared from human jurkat cells at 200000 cells

    Secondary
    HRP conjugated Donkey polyclonal to rabbit IgG at 1/2000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 22 kDa
    Observed band size: 22 kDa


    Exposure time: 30 seconds


    Primary diluted in PBS (5% BSA + 0.1% tween20) and incubated with sample for 1 hour and 30 minutes at 20°C.

    See Abreview

  • Anti-Bim antibody [Y36] (ab32158)
    Anti-Bim antibody [Y36] (ab32158)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (78)

Publishing research using ab32158? Please let us know so that we can cite the reference in this datasheet.

ab32158 has been referenced in 78 publications.

  • Xie W  et al. Single-nucleotide polymorphism rs4142441 and MYC co-modulated long non-coding RNA OSER1-AS1 suppresses non-small cell lung cancer by sequestering ELAVL1. Cancer Sci 112:2272-2286 (2021). PubMed: 33113263
  • Zhuang X  et al. All-Trans Retinoic Acid Attenuates Blue Light-Induced Apoptosis of Retinal Photoreceptors by Upregulating MKP-1 Expression. Mol Neurobiol 58:4157-4168 (2021). PubMed: 33950345
  • Chen L  et al. Mangiferin prevents myocardial infarction-induced apoptosis and heart failure in mice by activating the Sirt1/FoxO3a pathway. J Cell Mol Med 25:2944-2955 (2021). PubMed: 33523605
  • Rys RN  et al. Apoptotic Blocks in Primary Non-Hodgkin B Cell Lymphomas Identified by BH3 Profiling. Cancers (Basel) 13:N/A (2021). PubMed: 33670870
  • Yan S  et al. The effect of maternal high-fat-diet mediated oxidative stress on ovarian function in mice offspring. Exp Ther Med 20:135 (2020). PubMed: 33082867
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-7 of 7 Abreviews or Q&A

Western blot abreview for Anti-Bim antibody [Y36]

Poor
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Balb/C mice brain)
Gel Running Conditions
Reduced Denaturing (4-20% tris-glycin gel)
Loading amount
40 µg
Specification
Balb/C mice brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 08 2021

Western blot abreview for Anti-Bim antibody [Y36]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (SHSY5Y cells)
Gel Running Conditions
Reduced Denaturing (4-20% tris-glycin gel)
Loading amount
40 µg
Specification
SHSY5Y cells
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 03 2021

Immunoprecipitation abreview for Anti-Bim antibody [Y36]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Immuno-precipitation step
Protein A/G
Sample
Human Cell lysate - whole cell (HCT116 p53-/- cell line)
Specification
HCT116 p53-/- cell line
Total protein in input
100 µg
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Christian Marx

Verified customer

Submitted Aug 27 2013

Western blot abreview for Anti-Bim antibody [Y36]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF cell line)
Loading amount
60 µg
Specification
MEF cell line
Treatment
DMSO control and 2 µM SAHA for 24 hrs
Gel Running Conditions
Reduced Denaturing (13%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Christian Marx

Verified customer

Submitted Feb 13 2013

Western blot abreview for Anti-Bim antibody [Y36]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - other (HCT116 colon cancer cell line wt and p53 negative)
Loading amount
60 µg
Specification
HCT116 colon cancer cell line wt and p53 negative
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Christian Marx

Verified customer

Submitted Oct 26 2012

Western blot abreview for Anti-Bim antibody [Y36]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Jurkat cells)
Loading amount
200000 cells
Specification
Jurkat cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 24 2009

Question

Thanks for your reply. I had beta tubulin as the loading control which worked perfectly fine. So I do not think there is a problem with the cell lysate. I stored the antibody at -20 degree celsius afeter alliquoting it in order to avoid repeated freeze/thaw cycles. Unfortunately I do not have access to K562 or A549 whole cell lysates. I did an overnight incubation with the primary antibody at 4 degree celsius. I saw on your website that you also have a monoclonal antibody against BIM ( ab32158). I was wondering if you could send me that antibody ie Anti-BIm antibody (Y36) catalog number ab32158 as a replacement so that I can test it on my samples.

Read More

Abcam community

Verified customer

Asked on Dec 18 2012

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab32158.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Abcam Scientific Support

Answered on Dec 18 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.