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    products/primary-antibodies/bim-phospho-s65-antibody-ab17935.pdf

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Cell Biology Apoptosis Intracellular Associated Proteins
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Anti-Bim (phospho S65) antibody (ab17935)

  • Datasheet
  • SDS
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Western blot - Anti-Bim (phospho S65) antibody (ab17935)

    Key features and details

    • Rabbit polyclonal to Bim (phospho S65)
    • Suitable for: WB
    • Reacts with: Recombinant fragment
    • Isotype: IgG

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    Anti-Bcl-XL antibody [E18] (ab32370)

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    Overview

    • Product name

      Anti-Bim (phospho S65) antibody
      See all Bim primary antibodies
    • Description

      Rabbit polyclonal to Bim (phospho S65)
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Recombinant fragment
    • Immunogen

      Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

    • Positive control

      • Hek293T co-expressing rat Bim and active MEKK1
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7.30
      Preservative: 0.05% Sodium azide
      Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Bim. The final product is generated by affinity chromatography using a Bim-derived peptide that is phosphorylated at serine 65 (serine 69 in the human sequence).
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Apoptosis
      • Intracellular
      • Associated Proteins
      • Cell Biology
      • Apoptosis
      • Intracellular
      • Bcl2 Family
      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Alzheimer's disease
      • Other
      • Immunology
      • Innate Immunity
      • Macrophage / Inflamm.
      • Signal Transduction
      • Protein Phosphorylation
      • Ser / Thr Kinases
      • MAPK Pathway
      • Cancer
      • Invasion/microenvironment
      • Apoptosis
      • Bcl 2 family
      • Cancer
      • Oncoproteins/suppressors
      • Oncoproteins
      • Cell survival & death
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Apoptosis
      • Cancer
      • Cell Death
      • Apoptosis
      • Apoptosis Markers
      • Bcl 2 family
      • Cancer
      • Cell Death
      • Apoptosis
      • Metabolism

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Bim protein (ab112387)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab17935 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 17 kDa).
    Notes
    WB
    1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 17 kDa).

    Target

    • Function

      Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.
    • Tissue specificity

      Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney.
    • Sequence similarities

      Belongs to the Bcl-2 family.
    • Domain

      The BH3 motif is required for Bcl-2 binding and cytotoxicity.
    • Cellular localization

      Mitochondrion and Endomembrane system. Associated with intracytoplasmic membranes.
    • Target information above from: UniProt accession O43521 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • BCL2 like 11 antibody
      • B2L11_HUMAN antibody
      • BAM antibody
      • Bcl 2 interacting protein Bim antibody
      • Bcl 2 related ovarian death agonist antibody
      • Bcl-2-like protein 11 antibody
      • BCL2 interacting mediator of cell death antibody
      • BCL2 like 11 (apoptosis facilitator) antibody
      • BCL2 like protein 11 antibody
      • Bcl2-interacting mediator of cell death antibody
      • Bcl2-L-11 antibody
      • Bcl2l11 antibody
      • BIM alpha6 antibody
      • BIM antibody
      • BIM beta6 antibody
      • BIM beta7 antibody
      • BimEL antibody
      • BimL antibody
      • BOD antibody
      see all

    Images

    • Western blot - Anti-Bim (phospho S65) antibody (ab17935)
      Western blot - Anti-Bim (phospho S65) antibody (ab17935)

      Lysates prepared from Hek293T cells transfected with rat WT Bim alone (1, 4), cotransfected with rat WT Bim and activated MEKK1 (2, 5) or with
      mutant S65A Bim and MEKK1 (3, 6), were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were blocked with 3% Milk-TBST buffer for one hour at room temperature, and incubated with Bim Pan antibody (1, 2, 3) or ab17935 (4, 5, 6) for two hours at room temperature in 3% Milk-TBST buffer. After washing, membranes were incubated with a goat F(ab')2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal™ method.


      The data show that the signal is detected only in lysates co-expressing rat WT Bim and active MEKK1. This signal is abolished in cells expressing mutant rat Bim S65A and MEKK1, verifying that the signal is site-phosphospecific.

      Lysates prepared from Hek293T cells transfected with rat WT Bim alone (1, 4), cotransfected with rat WT Bim and activated MEKK1 (2

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (4)

    Publishing research using ab17935? Please let us know so that we can cite the reference in this datasheet.

    ab17935 has been referenced in 4 publications.

    • Wang L  et al. Danggui Buxue Tang Rescues Folliculogenesis and Ovarian Cell Apoptosis in Rats with Premature Ovarian Insufficiency. Evid Based Complement Alternat Med 2021:6614302 (2021). PubMed: 34035823
    • Li D  et al. Involvement of the JNK/FOXO3a/Bim Pathway in Neuronal Apoptosis after Hypoxic-Ischemic Brain Damage in Neonatal Rats. PLoS One 10:e0132998 (2015). IF ; Rat . PubMed: 26171786
    • Wang Y  et al. Tuberous sclerosis 1 (Tsc1)-dependent metabolic checkpoint controls development of dendritic cells. Proc Natl Acad Sci U S A 110:E4894-903 (2013). PubMed: 24282297
    • Wang Y  et al. Transforming growth factor beta-activated kinase 1 (TAK1)-dependent checkpoint in the survival of dendritic cells promotes immune homeostasis and function. Proc Natl Acad Sci U S A 109:E343-52 (2012). WB ; Mouse . PubMed: 22308391

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