Recombinant Anti-BIN1 antibody [EPR13463-25] (ab185950)
![Western blot - Anti-BIN1 antibody [EPR13463-25] (ab185950)](https://www.abcam.com/ps/products/185/ab185950/Images/ab185950-277386-anti-bin1-antibody-epr13463-25-western-blot.jpg "Western blot - Anti-BIN1 antibody [EPR13463-25] (ab185950)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13463-25] to BIN1
- Suitable for: ICC/IF, WB, IHC-P, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-BIN1 antibody [EPR13463-25]
See all BIN1 primary antibodies -
Description
Rabbit monoclonal [EPR13463-25] to BIN1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-P, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- U87-MG, Human fetal kidney and HeLa whole cell lysate (ab150035); Human fetal brain lysate;, Human skeletal muscle and mouse brain tissues; HeLa and U87-MG cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13463-25 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab185950 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100.
|
|
| WB | (1) |
1/1000. Detects a band of approximately 56, 65, 70, 90 kDa (predicted molecular weight: 65 kDa).
|
| IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| Flow Cyt (Intra) |
1/90.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
ICC/IF
1/100. |
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WB
1/1000. Detects a band of approximately 56, 65, 70, 90 kDa (predicted molecular weight: 65 kDa). |
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IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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Flow Cyt (Intra)
1/90. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation. -
Tissue specificity
Ubiquitous. Highest expression in the brain and muscle. Isoform IIA is expressed only in the brain where it is concentrated in axon initial segments and nodes of Ranvier. Isoform BIN1 is widely expressed with highest expression in skeletal muscle. -
Involvement in disease
Defects in BIN1 are the cause of centronuclear myopathy autosomal recessive (ARCNM) [MIM:255200]; also known as autosomal recessive myotubular myopathy. Centronuclear myopathies are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers. -
Sequence similarities
Contains 1 BAR domain.
Contains 1 SH3 domain. -
Post-translational
modificationsPhosphorylated by protein kinase C. -
Cellular localization
Cytoplasm and Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 274 Human
- Entrez Gene: 30948 Mouse
- Entrez Gene: 117028 Rat
- Omim: 601248 Human
- SwissProt: O00499 Human
- SwissProt: O08539 Mouse
- SwissProt: O08839 Rat
- Unigene: 193163 Human
see all -
Alternative names
- AMPH 2 antibody
- AMPH2 antibody
- Amphiphysin 2 antibody
see all
Images
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Western blot - Anti-BIN1 antibody [EPR13463-25] (ab185950)
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: BIN1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: U-87MG whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab185950 observed at 45-65 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab185950 was shown to specifically react with BIN1 when BIN1 knockout samples were used. Wild-type and BIN1 knockout samples were subjected to SDS-PAGE. Ab185950 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 µg/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] (ab185950)](https://www.abcam.com/ps/products/185/ab185950/Images/ab185950-219417-anti-bin1-antibody-epr13463-25-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] (ab185950)Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling BIN1 with ab185950 at 1/100 dilution. Secondary ab: Ready to use HRP Polymer for Rabbit IgG. Counter stain: Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] (ab185950)](https://www.abcam.com/ps/products/185/ab185950/Images/ab185950-219419-anti-bin1-antibody-epr13463-25-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] (ab185950)Immunofluorescent analysis of HeLa cells labeling BIN1 with ab185950 at 1/100 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Fixative: -20℃ Acetone. Counter stain: Dapi (blue).
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![Flow Cytometry (Intracellular) - Anti-BIN1 antibody [EPR13463-25] (ab185950)](https://www.abcam.com/ps/products/185/ab185950/Images/ab185950-219423-anti-bin1-antibody-epr13463-25-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-BIN1 antibody [EPR13463-25] (ab185950)Intracellular flow cytometric analysis ofHela cells labeling BIN1 using ab185950 at 1/90 dilution (red). Secondary ab: Goat anti rabbit IgG (FITC) at 1/150 dilution. Fixative: 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG (green).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] (ab185950)](https://www.abcam.com/ps/products/185/ab185950/Images/ab185950-219418-anti-bin1-antibody-epr13463-25-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] (ab185950)Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling BIN1 with ab185950 at 1/100 dilution. Secondary ab: Ready to use HRP Polymer for Rabbit IgG. Counter stain: Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Western blot - Anti-BIN1 antibody [EPR13463-25] (ab185950)](https://www.abcam.com/ps/products/185/ab185950/Images/ab185950-219413-anti-bin1-antibody-epr13463-25-western-blot.jpg)
Western blot - Anti-BIN1 antibody [EPR13463-25] (ab185950)All lanes : Anti-BIN1 antibody [EPR13463-25] (ab185950) at 1/1000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Hela cell lysate
Lane 4 : Human fetal brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 65 kDaBlocking buffer: 5% NFDM/TBST
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![Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] (ab185950)](https://www.abcam.com/ps/products/185/ab185950/Images/ab185950-219422-anti-bin1-antibody-epr13463-25-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] (ab185950)Immunofluorescent analysis of U87-MG cells labeling BIN1 with ab185950 at 1/100 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Fixative: 4% paraformaldehyde. Counter stain: Dapi (blue)
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![Anti-BIN1 antibody [EPR13463-25] (ab185950)](https://www.abcam.com/ps/products/185/ab185950/Images/ab185950-8-benefits-of-recombinant-antibodies.png)
Anti-BIN1 antibody [EPR13463-25] (ab185950)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab185950 has been referenced in 3 publications.
- Zheng J et al. microRNA-211 promotes proliferation, migration, and invasion ability of oral squamous cell carcinoma cells via targeting the bridging integrator 1 protein. J Cell Biochem 120:4644-4653 (2019). PubMed: 30260023
- Andrew RJ et al. Reduction of the expression of the late-onset Alzheimer's disease (AD) risk-factor BIN1 does not affect amyloid pathology in an AD mouse model. J Biol Chem 294:4477-4487 (2019). PubMed: 30692199
- McKenzie AT et al. Multiscale network modeling of oligodendrocytes reveals molecular components of myelin dysregulation in Alzheimer's disease. Mol Neurodegener 12:82 (2017). WB, IHC-P ; Mouse . PubMed: 29110684