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    products/primary-antibodies/bin1-antibody-epr13463-25-ab185950.pdf

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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-BIN1 antibody [EPR13463-25] (ab185950)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (3) Submit a question References (3)

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Western blot - Anti-BIN1 antibody [EPR13463-25] (ab185950)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] (ab185950)
  • Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] (ab185950)
  • Flow Cytometry (Intracellular) - Anti-BIN1 antibody [EPR13463-25] (ab185950)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] (ab185950)
  • Western blot - Anti-BIN1 antibody [EPR13463-25] (ab185950)
  • Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] (ab185950)
  • Anti-BIN1 antibody [EPR13463-25] (ab185950)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13463-25] to BIN1
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt (Intra)
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-BIN1 antibody [EPR13463-25]
    See all BIN1 primary antibodies
  • Description

    Rabbit monoclonal [EPR13463-25] to BIN1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, Flow Cyt (Intra)more details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • U87-MG, Human fetal kidney and HeLa whole cell lysate (ab150035); Human fetal brain lysate;, Human skeletal muscle and mouse brain tissues; HeLa and U87-MG cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13463-25
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Other
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • Other
    • Cancer
    • Cell cycle
    • Cell cycle inhibitors
    • Other
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other
    • Neuroscience
    • Diseases

Associated products

  • Alternative Versions

    • Anti-BIN1 antibody [EPR13463-25] - BSA and Azide free (ab226006)
  • Compatible Secondaries

    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
    • Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human BIN1 protein (ab98238)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab185950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100.
WB (1)
1/1000. Detects a band of approximately 56, 65, 70, 90 kDa (predicted molecular weight: 65 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt (Intra)
1/90.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Notes
ICC/IF
1/100.
WB
1/1000. Detects a band of approximately 56, 65, 70, 90 kDa (predicted molecular weight: 65 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt (Intra)
1/90.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation.
  • Tissue specificity

    Ubiquitous. Highest expression in the brain and muscle. Isoform IIA is expressed only in the brain where it is concentrated in axon initial segments and nodes of Ranvier. Isoform BIN1 is widely expressed with highest expression in skeletal muscle.
  • Involvement in disease

    Defects in BIN1 are the cause of centronuclear myopathy autosomal recessive (ARCNM) [MIM:255200]; also known as autosomal recessive myotubular myopathy. Centronuclear myopathies are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers.
  • Sequence similarities

    Contains 1 BAR domain.
    Contains 1 SH3 domain.
  • Post-translational
    modifications

    Phosphorylated by protein kinase C.
  • Cellular localization

    Cytoplasm and Nucleus.
  • Target information above from: UniProt accession O00499 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 274 Human
    • Entrez Gene: 30948 Mouse
    • Entrez Gene: 117028 Rat
    • Omim: 601248 Human
    • SwissProt: O00499 Human
    • SwissProt: O08539 Mouse
    • SwissProt: O08839 Rat
    • Unigene: 193163 Human
    • Unigene: 4383 Mouse
    • Unigene: 17098 Rat
    see all
  • Alternative names

    • AMPH 2 antibody
    • AMPH2 antibody
    • Amphiphysin 2 antibody
    • Amphiphysin II antibody
    • Amphiphysin like protein antibody
    • amphiphysin-like antibody
    • Amphiphysin-like protein antibody
    • AMPHL antibody
    • Bin1 antibody
    • BIN1_HUMAN antibody
    • Box Dependant MYC Interacting Protein 1 antibody
    • Box-dependent myc-interacting protein 1 antibody
    • Bridging integrator 1 antibody
    • DKFZp547F068 antibody
    • MGC10367 antibody
    • MGC105358 antibody
    • Myc box dependent interacting protein 1 antibody
    • Myc box-dependent-interacting protein 1 antibody
    • SH3P9 antibody
    see all

Images

  • Western blot - Anti-BIN1 antibody [EPR13463-25] (ab185950)
    Western blot - Anti-BIN1 antibody [EPR13463-25] (ab185950)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: BIN1 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: U-87MG whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab185950 observed at 45-65 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab185950 was shown to specifically react with BIN1 when BIN1 knockout samples were used. Wild-type and BIN1 knockout samples were subjected to SDS-PAGE. Ab185950 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 µg/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] (ab185950)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] (ab185950)

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling BIN1 with ab185950 at 1/100 dilution. Secondary ab: Ready to use HRP Polymer for Rabbit IgG. Counter stain: Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] (ab185950)
    Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] (ab185950)

    Immunofluorescent analysis of HeLa cells labeling BIN1 with ab185950 at 1/100 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Fixative: -20℃ Acetone. Counter stain: Dapi (blue).

  • Flow Cytometry (Intracellular) - Anti-BIN1 antibody [EPR13463-25] (ab185950)
    Flow Cytometry (Intracellular) - Anti-BIN1 antibody [EPR13463-25] (ab185950)

    Intracellular flow cytometric analysis ofHela cells labeling BIN1 using ab185950 at 1/90 dilution (red). Secondary ab: Goat anti rabbit IgG (FITC) at 1/150 dilution. Fixative: 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG (green). 

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] (ab185950)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] (ab185950)

    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling BIN1 with ab185950 at 1/100 dilution. Secondary ab: Ready to use HRP Polymer for Rabbit IgG. Counter stain: Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-BIN1 antibody [EPR13463-25] (ab185950)
    Western blot - Anti-BIN1 antibody [EPR13463-25] (ab185950)
    All lanes : Anti-BIN1 antibody [EPR13463-25] (ab185950) at 1/1000 dilution

    Lane 1 : U87-MG cell lysate
    Lane 2 : Human fetal kidney lysate
    Lane 3 : Hela cell lysate
    Lane 4 : Human fetal brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 65 kDa



    Blocking buffer: 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] (ab185950)
    Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] (ab185950)

    Immunofluorescent analysis of U87-MG cells labeling BIN1 with ab185950 at 1/100 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Fixative: 4% paraformaldehyde. Counter stain: Dapi (blue)

  • Anti-BIN1 antibody [EPR13463-25] (ab185950)
    Anti-BIN1 antibody [EPR13463-25] (ab185950)

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (3)

Publishing research using ab185950? Please let us know so that we can cite the reference in this datasheet.

ab185950 has been referenced in 3 publications.

  • Zheng J  et al. microRNA-211 promotes proliferation, migration, and invasion ability of oral squamous cell carcinoma cells via targeting the bridging integrator 1 protein. J Cell Biochem 120:4644-4653 (2019). PubMed: 30260023
  • Andrew RJ  et al. Reduction of the expression of the late-onset Alzheimer's disease (AD) risk-factor BIN1 does not affect amyloid pathology in an AD mouse model. J Biol Chem 294:4477-4487 (2019). PubMed: 30692199
  • McKenzie AT  et al. Multiscale network modeling of oligodendrocytes reveals molecular components of myelin dysregulation in Alzheimer's disease. Mol Neurodegener 12:82 (2017). WB, IHC-P ; Mouse . PubMed: 29110684

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Western blot abreview for Anti-BIN1 antibody [EPR13463-25]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
30 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 17 2020

Immunohistochemistry (Frozen sections) abreview for Anti-BIN1 antibody [EPR13463-25]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart)
Permeabilization
Yes - Tween-20
Specification
Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 11 2020

Immunohistochemistry (Frozen sections) abreview for Anti-BIN1 antibody [EPR13463-25]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - Triton X-100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 07 2016

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