Recombinant Anti-Biotin antibody [BTN/2032R] (ab234284)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biotin antibody [BTN/2032R] (ab234284)](https://www.abcam.com/ps/products/234/ab234284/Images/ab234284-308356-anti-biotin-antibody-btn-2032r-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biotin antibody [BTN/2032R] (ab234284)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BTN/2032R] to Biotin
- Suitable for: IHC-P
Related conjugates and formulations
Overview
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Product name
Anti-Biotin antibody [BTN/2032R]
See all Biotin primary antibodies -
Description
Rabbit monoclonal [BTN/2032R] to Biotin -
Host species
Rabbit -
Specificity
ab234284 recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. The antibody is highly specific to biotin and shows no cross-reaction with other structurally related compounds. -
Tested applications
Suitable for: IHC-Pmore details -
Immunogen
Chemical/ Small Molecule corresponding to Biotin. (Biotinylated sheep immunoglobulin).
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Positive control
- IHC-P: Human tonsil tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
BTN/2032R -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 0.5 - 1 µg/ml.
(Primary incubation for 10 minutes at room temperature). |
| Notes |
|---|
|
IHC-P
Use a concentration of 0.5 - 1 µg/ml. (Primary incubation for 10 minutes at room temperature). |
Target
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Relevance
Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da. -
Alternative names
- Vitamin B4 antibody
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biotin antibody [BTN/2032R] (ab234284)
Formalin-fixed, paraffin-embedded human tonsil tissue stained with biotinylated Kappa Light Chain probe and then for Biotin using ab234284 at 1 μg/ml in immunohistochemical analysis.
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![Anti-Biotin antibody [BTN/2032R] (ab234284)](https://www.abcam.com/ps/products/234/ab234284/Images/ab234284-2-benefits-of-recombinant-antibodies.png)
Anti-Biotin antibody [BTN/2032R] (ab234284)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab234284 has been referenced in 1 publication.
- Paldor M et al. Single-cell transcriptomics reveals a senescence-associated IL-6/CCR6 axis driving radiodermatitis. EMBO Mol Med 14:e15653 (2022). PubMed: 35785521
