Anti-Biotin antibody [Hyb-8] (ab201341)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biotin antibody [Hyb-8] (ab201341)](https://www.abcam.com/ps/products/201/ab201341/Images/ab201341-243025-anti-biotin-antibody-hyb-8-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biotin antibody [Hyb-8] (ab201341)")
Key features and details
- Mouse monoclonal [Hyb-8] to Biotin
- Suitable for: Flow Cyt, In situ hybridization, WB, ICC/IF, IHC-P
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-Biotin antibody [Hyb-8]
See all Biotin primary antibodies -
Description
Mouse monoclonal [Hyb-8] to Biotin -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, In situ hybridization, WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Biotin. (Biotinylated sheep immunoglobulin).
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Positive control
- Biotinylated proteins in solution or on tissues.
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General notes
ab201341 has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
ab201341 is purified from Bioreactor Concentrate by Protein A/G column chromatography. -
Clonality
Monoclonal -
Clone number
Hyb-8 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab201341 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 0.5-1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| In situ hybridization |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration.
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| ICC/IF |
Use a concentration of 0.5 - 1 µg/ml.
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| IHC-P |
Use a concentration of 0.5 - 1 µg/ml.
No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues. |
| Notes |
|---|
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Flow Cyt
Use 0.5-1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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In situ hybridization
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. |
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ICC/IF
Use a concentration of 0.5 - 1 µg/ml. |
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IHC-P
Use a concentration of 0.5 - 1 µg/ml. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues. |
Target
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Relevance
Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da. -
Alternative names
- Vitamin B4 antibody
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biotin antibody [Hyb-8] (ab201341)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue stained with biotinylated Lambda Light Chain probe followed by ab201341 at 1 µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab201341 has been referenced in 10 publications.
- Wang YC et al. Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA ubiquitination. Cell Rep 42:112296 (2023). PubMed: 36961817
- Domen A et al. Prognostic implications of cellular senescence in resected non-small cell lung cancer. Transl Lung Cancer Res 11:1526-1539 (2022). PubMed: 36090630
- Oo JA et al. Long non-coding RNA PCAT19 safeguards DNA in quiescent endothelial cells by preventing uncontrolled phosphorylation of RPA2. Cell Rep 41:111670 (2022). PubMed: 36384122
- Kohli J et al. Targeting anti-apoptotic pathways eliminates senescent melanocytes and leads to nevi regression. Nat Commun 13:7923 (2022). PubMed: 36564381
- Zepecki JP et al. miRNA-mediated loss of m6A increases nascent translation in glioblastoma. PLoS Genet 17:e1009086 (2021). PubMed: 33684100
- Kohli J et al. Algorithmic assessment of cellular senescence in experimental and clinical specimens. Nat Protoc 16:2471-2498 (2021). PubMed: 33911261
- Kulkarni NN et al. Sequence determinants in the cathelicidin LL-37 that promote inflammation via presentation of RNA to scavenger receptors. J Biol Chem 297:100828 (2021). PubMed: 34048712
- Shaw A et al. Binding to nanopatterned antigens is dominated by the spatial tolerance of antibodies. Nat Nanotechnol 14:184-190 (2019). PubMed: 30643273
- Powell ML et al. New Fpg probe chemistry for direct detection of recombinase polymerase amplification on lateral flow strips. Anal Biochem 543:108-115 (2018). PubMed: 29233680
- Roussis IM et al. RNA Whole-Mount In situ Hybridisation Proximity Ligation Assay (rISH-PLA), an Assay for Detecting RNA-Protein Complexes in Intact Cells. PLoS One 11:e0147967 (2016). PubMed: 26824753