Biotin Anti-C5b-9 + C5b-8 antibody [aE11] (ab237699)
Key features and details
- Biotin Mouse monoclonal [aE11] to C5b-9 + C5b-8
- Suitable for: ELISA
- Reacts with: Horse, Human, Pig, Baboon
- Conjugation: Biotin
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
Biotin Anti-C5b-9 + C5b-8 antibody [aE11] -
Description
Biotin Mouse monoclonal [aE11] to C5b-9 + C5b-8 -
Host species
Mouse -
Conjugation
Biotin -
Specificity
ab237699 binds both membrane-bound MAC (active) and fluid-phase SC5b-9 complexes (inactive).
The aE11 monoclonal was first reported to bind to a neoepitope exposed on polymerized C9 when incorporated into the human terminal complement complex (PubMed ID: 4035298). It was subsequently shown that this antibody also cross reacts with the C5b-8 complex and purified C8 alpha-gamma. It does not react with native C9 or C8 proteins (PubMed ID: 2424021).
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Tested applications
Suitable for: ELISAmore details -
Species reactivity
Reacts with: Horse, Human, Pig, Baboon -
Immunogen
Full length native protein (purified) corresponding to Human C5b-9.
Database link: P01031 -
Epitope
ab237699 binds to a neoepitope exposed on C9. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.0975% Sodium azide
Constituents: 0.16% Sodium phosphate, 0.81% Sodium chloride -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Biotinylated -
Clonality
Monoclonal -
Clone number
aE11 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab237699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| Notes |
|---|
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ELISA
Use at an assay dependent concentration. |
Target
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Cellular localization
C5b-9: Secreted -
Database links
- Entrez Gene: 727 Human
- Entrez Gene: 729 Human
- Entrez Gene: 730 Human
- Entrez Gene: 731 Human
- Entrez Gene: 735 Human
- SwissProt: P01031 Human
- SwissProt: P02748 Human
- SwissProt: P07357 Human
see all
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab237699 has not yet been referenced specifically in any publications.