Biotin Anti-CD34 antibody [4H11[APG]] (ab21893)
![Flow Cytometry - Biotin Anti-CD34 antibody [4H11[APG]] (ab21893)](https://www.abcam.com/ps/products/21/ab21893/Images/ab21893-455622-biotin-anti-cd34-antibody-4h11-apg-flow-cytometry-peripheral-blood-human.jpg "Flow Cytometry - Biotin Anti-CD34 antibody [4H11[APG]] (ab21893)")
Key features and details
- Biotin Mouse monoclonal [4H11[APG]] to CD34
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG1
Overview
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Product name
Biotin Anti-CD34 antibody [4H11[APG]]
See all CD34 primary antibodies -
Description
Biotin Mouse monoclonal [4H11[APG]] to CD34 -
Host species
Mouse -
Conjugation
Biotin -
Specificity
The antibody 4H11[APG] completely blocks binding of Class II antibody QBEnd10 and Class III antibodies BIRMA K3 and 8G12 on KG1a cell line. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to CD34. Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia
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Epitope
Class III epitope on CD34 antigen. -
Positive control
- Flow Cyt: Human peripheral blood.
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General notes
The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
4H11[APG] -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab21893 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use a concentration of 1 - 2 µg/ml.
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| Notes |
|---|
|
Flow Cyt
Use a concentration of 1 - 2 µg/ml. |
Target
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Function
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. -
Tissue specificity
Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. -
Sequence similarities
Belongs to the CD34 family. -
Developmental stage
On early hematopoietic progenitor cells. -
Post-translational
modificationsHighly glycosylated.
Phosphorylated on serine residues by PKC. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 947 Human
- Omim: 142230 Human
- SwissProt: P28906 Human
- Unigene: 374990 Human
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Alternative names
- CD34 antibody
- CD34 antigen antibody
- CD34 molecule antibody
see all
Images
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Flow Cytometry - Biotin Anti-CD34 antibody [4H11[APG]] (ab21893)
Flow Cytometry analysis of human peripheral blood labeling CD34 positive CD45dim stem cells with ab21893 (red-filled) from lymphocytes (black-dashed) and visualized with biotin / streptavidin-APC.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab21893 has not yet been referenced specifically in any publications.