Biotin Anti-CD42b antibody [AK2] (ab30400)
Key features and details
- Biotin Mouse monoclonal [AK2] to CD42b
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG1
Overview
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Product name
Biotin Anti-CD42b antibody [AK2]
See all CD42b primary antibodies -
Description
Biotin Mouse monoclonal [AK2] to CD42b -
Host species
Mouse -
Conjugation
Biotin -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Human peripheral blood platelets
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General notes
Clone AK2 has been reported to block the binding of von Willebrand Factor to platelets.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Primary antibody notes
Clone AK2 has been reported to block the binding of von Willebrand Factor to platelets. -
Clonality
Monoclonal -
Clone number
AK2 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab30400 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
Use neat or 10µl for 100µl of whole blood.
ab18434 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Notes |
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Flow Cyt
Use at an assay dependent concentration. Use neat or 10µl for 100µl of whole blood.
ab18434 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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Function
GP-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium. -
Involvement in disease
Non-arteritic anterior ischemic optic neuropathy
Bernard-Soulier syndrome
Bernard-Soulier syndrome A2, autosomal dominant
Pseudo-von Willebrand disease -
Sequence similarities
Contains 7 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain. -
Post-translational
modificationsGlycocalicin, which is approximately coextensive with the extracellular part of the molecule, is cleaved off by calpain during platelet lysis. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 2811 Human
- Omim: 606672 Human
- SwissProt: P07359 Human
- Unigene: 1472 Human
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Alternative names
- Antigen CD42b alpha antibody
- Antigen CD42b-alpha antibody
- BDPLT1 antibody
see all
Images
Datasheets and documents
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Datasheet download
References (2)
ab30400 has been referenced in 2 publications.
- Montoro-García S et al. Small-size platelet microparticles trigger platelet and monocyte functionality and modulate thrombogenesis via P-selectin. Br J Haematol 166:571-80 (2014). PubMed: 24766273
- Kumar RA et al. Kinetics of GPIbalpha-vWF-A1 tether bond under flow: effect of GPIbalpha mutations on the association and dissociation rates. Biophys J 85:4099-109 (2003). PubMed: 14645097