Biotin Anti-CD63 antibody [MEM-259] (ab134331)
![Flow Cytometry - Biotin Anti-CD63 antibody [MEM-259] (ab134331)](https://www.abcam.com/ps/products/134/ab134331/Images/ab134331-449635-anti-cd63-antibody-flow-cytometry-peripheral-blood-human.jpg "Flow Cytometry - Biotin Anti-CD63 antibody [MEM-259] (ab134331)")
Key features and details
- Biotin Mouse monoclonal [MEM-259] to CD63
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Biotin Anti-CD63 antibody [MEM-259]
See all CD63 primary antibodies -
Description
Biotin Mouse monoclonal [MEM-259] to CD63 -
Host species
Mouse -
Conjugation
Biotin -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD63. HPB-ALL T cell line
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Positive control
- Activated platelets, neutrophils and basophils. Human spleen tissue.
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General notes
The antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
MEM-259 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134331 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
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| Notes |
|---|
|
Flow Cyt
Use at an assay dependent concentration. |
Target
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Function
This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation. -
Tissue specificity
Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Cellular localization
Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules. - Information by UniProt
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Database links
- Entrez Gene: 967 Human
- Omim: 155740 Human
- SwissProt: P08962 Human
- Unigene: 445570 Human
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Alternative names
- Lysosomal associated membrane protein 3 antibody
- CD 63 antibody
- CD63 antibody
see all
Images
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Flow Cytometry - Biotin Anti-CD63 antibody [MEM-259] (ab134331)
Flow cytometry multicolor surface staining of IgE stimulated peripheral blood mononuclear cells stained usingab134331 (concentration in sample 0.6 μg/mL, Streptavidin APC).
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![Flow Cytometry - Biotin Anti-CD63 antibody [MEM-259] (ab134331)](https://www.abcam.com/ps/products/134/ab134331/Images/ab134331-449633-anti-cd63-antibody-flow-cytometry-peripheral-blood-human1.jpg)
Flow Cytometry - Biotin Anti-CD63 antibody [MEM-259] (ab134331)Flow cytometry multicolor surface staining of IgE stimulated peripheral blood mononuclear cells stained usingab134331 (concentration in sample 0.6 μg/mL, Streptavidin APC) and anti-human CD203c PE antibody (20 μL reagent / 100 μL of peripheral whole blood).
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![Flow Cytometry - Biotin Anti-CD63 antibody [MEM-259] (ab134331)](https://www.abcam.com/ps/products/134/ab134331/Images/ab134331-449632-anti-cd63-antibody-flow-cytometry-basophil-granulocytes-human.jpg)
Flow Cytometry - Biotin Anti-CD63 antibody [MEM-259] (ab134331)Flow Cytometry analysis of (surface staining) of IgE stimulated human peripheral whole blood using ab134331 (concentration in sample 0.6 μg/mL, Streptavidin APC). CD63 positive CD203c positive basophil granulocytes (red-filled) from neutrophil granulocytes (black-dashed).
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab134331 has been referenced in 6 publications.
- Temperini ME et al. Antenna-enhanced mid-infrared detection of extracellular vesicles derived from human cancer cell cultures. J Nanobiotechnology 20:530 (2022). PubMed: 36514065
- Wang JM et al. Comparative evaluation of methods for isolating small extracellular vesicles derived from pancreatic cancer cells. Cell Biosci 11:37 (2021). PubMed: 33568197
- Neo SY et al. CD73 immune checkpoint defines regulatory NK cells within the tumor microenvironment. J Clin Invest 130:1185-1198 (2020). PubMed: 31770109
- Zhang W et al. Enabling Sensitive Phenotypic Profiling of Cancer-Derived Small Extracellular Vesicles Using Surface-Enhanced Raman Spectroscopy Nanotags. ACS Sens 5:764-771 (2020). PubMed: 32134252
- Fan Z et al. Exosome-specific tumor diagnosis via biomedical analysis of exosome-containing microRNA biomarkers. Analyst 144:5856-5865 (2019). PubMed: 31482867
- Huang L et al. A dual-signal amplification platform for sensitive fluorescence biosensing of leukemia-derived exosomes. Nanoscale 10:20289-20295 (2018). PubMed: 30371719