Biotin Anti-CD9 antibody [A1501] (ab286172)
Key features and details
- Biotin Mouse monoclonal [A1501] to CD9
- Suitable for: WB, ELISA, IHC-Fr, IP
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG1
Overview
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Product name
Biotin Anti-CD9 antibody [A1501]
See all CD9 primary antibodies -
Description
Biotin Mouse monoclonal [A1501] to CD9 -
Host species
Mouse -
Conjugation
Biotin -
Specificity
WB (1:1000), ELISA (1:500), IHC, IP
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Tested applications
Suitable for: WB, ELISA, IHC-Fr, IPmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: COLO1 cell lysates, Plasma purified exosomes ELISA: purified exosomes from human plasma (PEP), urine (PEU)
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General notes
This product is manufactured by BioVision, an Abcam company and was previously called A1501 Anti-human CD9 biotin conjugated antibody. A1501-50 is the same size as the 50 µg size of ab286172.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: 99.9% PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified from tissue culture supernatant or ascites by protein A affinity chromatography. -
Clonality
Monoclonal -
Clone number
A1501 -
Isotype
IgG1 -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab286172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Use under non reducing condition. Predicted molecular weight: 24 kDa.
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ELISA |
1/500.
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IHC-Fr |
Use a concentration of 2 - 10 µg/ml.
This information is only intended as a guide. The optimal dilutions must be determined by the user. |
|
IP |
Use at an assay dependent concentration.
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Notes |
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WB
1/1000. Use under non reducing condition. Predicted molecular weight: 24 kDa. |
ELISA
1/500. |
IHC-Fr
Use a concentration of 2 - 10 µg/ml. This information is only intended as a guide. The optimal dilutions must be determined by the user. |
IP
Use at an assay dependent concentration. |
Target
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Function
Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. -
Tissue specificity
Expressed by a variety of hematopoietic and epithelial cells. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Post-translational
modificationsProtein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 928 Human
- Omim: 143030 Human
- SwissProt: P21926 Human
- Unigene: 114286 Human
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Alternative names
- Tetraspanin 29 antibody
- 5H9 antibody
- 5H9 antigen antibody
see all
Images
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All lanes : Biotin Anti-CD9 antibody [A1501] (ab286172)
Lane 1 : COLO1 cell lysates
Lane 2 : Plasma purified exosomes
Lysates/proteins at 20 µg per lane.
Performed under non-reducing conditions.
Predicted band size: 24 kDaThis information is only intended as a guide. The optimal dilutions must be determined by the user.
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CD9 detection in purified exosomes from human plasma (PEP) and urine (PEU), 30 µg.
This information is only intended as a guide. The optimal dilutions must be determined by the user.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab286172 has not yet been referenced specifically in any publications.