Biotin Anti-HLA Class I antibody [W6/32] (ab110665)
Key features and details
- Biotin Mouse monoclonal [W6/32] to HLA Class I
- Suitable for: Flow Cyt
- Reacts with: Cow, Cat, Human, Non human primates
- Conjugation: Biotin
- Isotype: IgG2a
Related conjugates and formulations
Overview
-
Product name
Biotin Anti-HLA Class I antibody [W6/32]
See all HLA Class I primary antibodies -
Description
Biotin Mouse monoclonal [W6/32] to HLA Class I -
Host species
Mouse -
Conjugation
Biotin -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Cow, Cat, Human, Non human primates
Does not react with: Rabbit -
Immunogen
Tissue, cells or virus corresponding to Human HLA Class I. Membrane of human tonsil cells
-
General notes
Suitable as a positive control for HLA tissue typing and crossmatching.
The purified antibody (>95% by SDS-PAGE) is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Size exclusion -
Primary antibody notes
Suitable as a positive control for HLA tissue typing and crossmatching. -
Clonality
Monoclonal -
Clone number
W6/32 -
Isotype
IgG2a -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110665 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use a concentration of 1 - 12 µg/ml.
|
Notes |
---|
Flow Cyt
Use a concentration of 1 - 12 µg/ml. |
Target
-
Relevance
HLA CLass I is involved in the presentation of foreign antigens to the immune system. -
Cellular localization
Plasma membrane -
Database links
- Entrez Gene: 3105 Human
- Entrez Gene: 3133 Human
- Entrez Gene: 3134 Human
- Entrez Gene: 3135 Human
- Omim: 142800 Human
- SwissProt: O15506 Human
- SwissProt: P04439 Human
- SwissProt: Q5MCQ6 Human
see all -
Alternative names
- DKFZp686P19218 antibody
- EA1.2 antibody
- EA2.1 antibody
see all
Images
-
Flow cytometry surface staining pattern of human peripheral whole blood stained using ab110665 at 4 μg/ml.
-
Separation of human leukocytes (red-filled) from HLA Class I negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood using ab110665 at 4 μg/ml.
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab110665 has been referenced in 4 publications.
- Wang D et al. Targeted Disruption of the ß2-Microglobulin Gene Minimizes the Immunogenicity of Human Embryonic Stem Cells. Stem Cells Transl Med 4:1234-45 (2015). PubMed: 26285657
- Choudhuri K et al. Peptide-major histocompatibility complex dimensions control proximal kinase-phosphatase balance during T cell activation. J Biol Chem 284:26096-105 (2009). PubMed: 19628870
- Tran TM et al. The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex. Immunogenetics 53:440-6 (2001). PubMed: 11685454
- Kievits F & Ivanyi P Monomorphic anti-HLA monoclonal antibody (W6/32) recognizes polymorphic H-2 heavy-chain determinants exposed by association with bovine or human but not murine beta 2-microglobulin. Hum Immunol 20:115-26 (1987). PubMed: 3316135