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    products/primary-antibodies/biotin-human-serum-albumin-antibody-ab24207.pdf

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Cardiovascular Blood Serum Proteins
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Biotin Anti-Human Serum Albumin antibody (ab24207)

  • Datasheet
  • SDS
Reviews (1)Q&A (4)References (2)

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Western blot - Biotin Anti-Human Serum Albumin antibody (ab24207)

    Key features and details

    • Biotin Rabbit polyclonal to Human Serum Albumin
    • Suitable for: ELISA, IHC-P, WB, ICC/IF
    • Reacts with: Human
    • Conjugation: Biotin
    • Isotype: IgG

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    Overview

    • Product name

      Biotin Anti-Human Serum Albumin antibody
      See all Human Serum Albumin primary antibodies
    • Description

      Biotin Rabbit polyclonal to Human Serum Albumin
    • Host species

      Rabbit
    • Conjugation

      Biotin
    • Tested applications

      Suitable for: ELISA, IHC-P, WB, ICC/IFmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Full length protein corresponding to Human Human Serum Albumin.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.01% Sodium azide
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% Polyethylene glycol
    • Concentration information loading...
    • Purity

      Protein A purified
    • Purification notes

      Purified from monospecific antiserum by Protein A Sepharose Fast Flow followed by POROS EP antigen resin affinity purification. The eluate was conjugated to biotin, re-purified, and then dialyzed against PBS with sodium azide prior to lyophilization.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cardiovascular
      • Blood
      • Serum Proteins
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Metabolism of lipids and lipoproteins
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Lipid metabolism
      • Metabolism
      • Types of disease
      • Cancer

    Associated products

    • Isotype control

      • Biotin Rabbit IgG - Isotype Control (ab200208)
    • Recombinant Protein

      • Recombinant Human Serum Albumin protein (His tag) (ab217817)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab24207 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ELISA (1)
    Use at an assay dependent concentration.
    IHC-P
    Use at an assay dependent concentration.
    WB
    1/1000 - 1/5000. Predicted molecular weight: 69 kDa.
    ICC/IF
    Use at an assay dependent concentration.
    Notes
    ELISA
    Use at an assay dependent concentration.
    IHC-P
    Use at an assay dependent concentration.
    WB
    1/1000 - 1/5000. Predicted molecular weight: 69 kDa.
    ICC/IF
    Use at an assay dependent concentration.

    Target

    • Function

      Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
    • Tissue specificity

      Plasma.
    • Involvement in disease

      Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
    • Sequence similarities

      Belongs to the ALB/AFP/VDB family.
      Contains 3 albumin domains.
    • Post-translational
      modifications

      Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
      Glycated in diabetic patients.
      Phosphorylation sites are present in the extracelllular medium.
      Acetylated on Lys-223 by acetylsalicylic acid.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P02768 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 213 Human
      • Omim: 103600 Human
      • SwissProt: P02768 Human
      • Unigene: 418167 Human
      • Alternative names

        • alb antibody
        • ALBU_HUMAN antibody
        • Albumin (32 AA) antibody
        • Albumin (AA 34) antibody
        • Albumin antibody
        • Analbuminemia antibody
        • Bisalbuminemia antibody
        • Cell growth inhibiting protein 42 antibody
        • DKFZp779N1935 antibody
        • Dysalbuminemic hyperthyroxinemia antibody
        • Growth inhibiting protein 20 antibody
        • HSA antibody
        • Hyperthyroxinemia dysalbuminemic antibody
        • PRO0883 antibody
        • PRO0903 antibody
        • PRO1341 antibody
        • Serum albumin antibody
        see all

      Images

      • Western blot - Biotin Anti-Human Serum Albumin antibody (ab24207)
        Western blot - Biotin Anti-Human Serum Albumin antibody (ab24207)
        Biotin Anti-Human Serum Albumin antibody (ab24207) at 1/1000 dilution + Human Serum Albumin at 0.05 µg

        Secondary
        HRP Streptavidin at 1/40000 dilution

        Predicted band size: 69 kDa
        Observed band size: 61 kDa why is the actual band size different from the predicted?

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (2)

      Publishing research using ab24207? Please let us know so that we can cite the reference in this datasheet.

      ab24207 has been referenced in 2 publications.

      • Wang CH & Lee GB Screening of multiple hemoprotein-specific aptamers and their applications for the binding, quantification, and extraction of hemoproteins in a microfluidic system. Biomicrofluidics 14:024110 (2020). PubMed: 32549920
      • Tallman KA  et al. Phospholipid-protein adducts of lipid peroxidation: synthesis and study of new biotinylated phosphatidylcholines. Chem Res Toxicol 20:227-34 (2007). PubMed: 17305406

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-5 of 5 Abreviews or Q&A

      ELISA abreview for Anti-Human Serum Albumin antibody (Biotin)

      Good
      Abreviews
      Abreviews
      Application
      ELISA
      Sample
      Human Purified protein (Sigma product, cat no A-1653)
      Specification
      Sigma product, cat no A-1653
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 2
      Type
      Indirect
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Apr 11 2006

      Question

      I am trying to find various lots that go back as far as 2009 for purchase. I would also like to purchase multiple lots for this cat #. We are trying to see if there is a difference that is creating an issue with our assay. There are time constraints under which I will have to have this information and order the product. I would appreciate a call abck ASAP. Thank You

      Read More

      Abcam community

      Verified customer

      Asked on Oct 24 2012

      Answer

      Thank you for your patience whilst I tried to find the information you requested.

      I have talked to the lab and they have informed me that this polyclonal antibody was product against whole human serum albumin (HSA) and is tested against HSA by ELISA using Streptavidin HRP and TMBE for detection. By our testing a titer between 150K and 200K was obtained. This product also showed a single precipitin arc when tested by IEP against human serum, human serum albumin, anti biotin, and anti rabbit serum. The product was not tested against BSA.

      I am sorry that I could not help anymore with this particular enquiry but if there is anything else I can help you with, please let me know.

      Read More

      Abcam Scientific Support

      Answered on Oct 24 2012

      Question

      Thanks for the information.



      Do you have any information that you can send me regarding affinity or spec sheets for the following lots of material: *

      Read More

      Abcam community

      Verified customer

      Asked on Oct 23 2012

      Answer

      Thank you for your reply.

      I looked into all of the lot numbers that you provided and all of those lots actually came from the same master lot ie from the same batch. They were just aliquoted and put into stock at different times, hence the different lot numbers that were on the vials that you received.

      I cannot find any information about any issues about decreased specificity or affinity for the different aliquots that you received.

      If there is anything else I can help you with, or if you are experiencing any issues with the antibody that I could provide technical assistance with, then please let me know.

      Read More

      Abcam Scientific Support

      Answered on Oct 23 2012

      Question

      I am trying to find various lots that go back as far as 2009 for purchase. I would also like to purchase multiple lots for this cat #. We are trying to see if there is a difference that is creating an issue with our assay. There are time constraints under which I will have to have this information and order the product. I would appreciate a call abck ASAP. Thank You

      Read More

      Abcam community

      Verified customer

      Asked on Oct 22 2012

      Answer

      Thank you for contacting Abcam.

      I have looked at the current stock of ab24207 and the only lot that we have is one that came into stock in June 2013 (lot# --). We do not have any other lots available.

      I am sorry that I could not be of any more assistance in this matter, but please let me know if you have any further questions.

      Read More

      Abcam Scientific Support

      Answered on Oct 22 2012

      Question

      Can bovine serum albumin be used as a blocking agent with ab24207 or will it inhibit the antibody binding to human serum albumin?

      Read More

      Abcam community

      Verified customer

      Asked on Mar 26 2006

      Answer

      Thank you for your enquiry. Furhter to correspondence with the lab I have determined that BSA can be used as a blocking agent with this antibody. The conjugate itself is supplied with 10mg/mL of BSA as a stablizer. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      Answered on Mar 29 2006

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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