Biotin Anti-Human Serum Albumin antibody (ab24207)
Key features and details
- Biotin Rabbit polyclonal to Human Serum Albumin
- Suitable for: ELISA, IHC-P, WB, ICC/IF
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
Overview
-
Product name
Biotin Anti-Human Serum Albumin antibody
See all Human Serum Albumin primary antibodies -
Description
Biotin Rabbit polyclonal to Human Serum Albumin -
Host species
Rabbit -
Conjugation
Biotin -
Tested applications
Suitable for: ELISA, IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein corresponding to Human Human Serum Albumin.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% Polyethylene glycol -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from monospecific antiserum by Protein A Sepharose Fast Flow followed by POROS EP antigen resin affinity purification. The eluate was conjugated to biotin, re-purified, and then dialyzed against PBS with sodium azide prior to lyophilization. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab24207 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | (1) |
Use at an assay dependent concentration.
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 69 kDa.
|
|
| ICC/IF |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
|
WB
1/1000 - 1/5000. Predicted molecular weight: 69 kDa. |
|
ICC/IF
Use at an assay dependent concentration. |
Target
-
Function
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. -
Tissue specificity
Plasma. -
Involvement in disease
Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. -
Sequence similarities
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
Post-translational
modificationsKenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 213 Human
- Omim: 103600 Human
- SwissProt: P02768 Human
- Unigene: 418167 Human
-
Alternative names
- alb antibody
- ALBU_HUMAN antibody
- Albumin (32 AA) antibody
see all
Images
-
Western blot - Biotin Anti-Human Serum Albumin antibody (ab24207)Biotin Anti-Human Serum Albumin antibody (ab24207) at 1/1000 dilution + Human Serum Albumin at 0.05 µg
Secondary
HRP Streptavidin at 1/40000 dilution
Predicted band size: 69 kDa
Observed band size: 61 kDa why is the actual band size different from the predicted?
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab24207 has been referenced in 2 publications.
- Wang CH & Lee GB Screening of multiple hemoprotein-specific aptamers and their applications for the binding, quantification, and extraction of hemoproteins in a microfluidic system. Biomicrofluidics 14:024110 (2020). PubMed: 32549920
- Tallman KA et al. Phospholipid-protein adducts of lipid peroxidation: synthesis and study of new biotinylated phosphatidylcholines. Chem Res Toxicol 20:227-34 (2007). PubMed: 17305406