Biotin Anti-Human Serum Albumin antibody [AL-01] (ab81426)
![Western blot - Biotin Anti-Human Serum Albumin antibody [AL-01] (ab81426)](https://www.abcam.com/ps/products/81/ab81426/Images/ab81426-395137-AntiHuman-Serum-Albumin-antibody-AL01-Biotin-western-blot-plasma-human.jpg "Western blot - Biotin Anti-Human Serum Albumin antibody [AL-01] (ab81426)")
Key features and details
- Biotin Mouse monoclonal [AL-01] to Human Serum Albumin
- Suitable for: WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG1
Overview
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Product name
Biotin Anti-Human Serum Albumin antibody [AL-01]
See all Human Serum Albumin primary antibodies -
Description
Biotin Mouse monoclonal [AL-01] to Human Serum Albumin -
Host species
Mouse -
Conjugation
Biotin -
Specificity
Reacts only with Human Serum Albumin. No cross-reactivity was observed with other serum proteins. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Does not react with: Mammals -
Immunogen
Full length native protein (purified) corresponding to Human Human Serum Albumin. Fraction of proteins containing Albumin after ammonnium sulphate precipitation and DEAE-chromatography of Human serum.
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Positive control
- WB: Human plasma.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
AL-01 -
Isotype
IgG1 -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab81426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 2 - 5 µg/ml. Predicted molecular weight: 69 kDa.
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| Notes |
|---|
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WB
Use a concentration of 2 - 5 µg/ml. Predicted molecular weight: 69 kDa. |
Target
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Function
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. -
Tissue specificity
Plasma. -
Involvement in disease
Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. -
Sequence similarities
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
Post-translational
modificationsKenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 213 Human
- Omim: 103600 Human
- SwissProt: P02768 Human
- Unigene: 418167 Human
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Alternative names
- alb antibody
- ALBU_HUMAN antibody
- Albumin (32 AA) antibody
see all
Images
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Western blot - Biotin Anti-Human Serum Albumin antibody [AL-01] (ab81426)All lanes : Biotin Anti-Human Serum Albumin antibody [AL-01] (ab81426) at 5 µg/ml
Lane 1 : Human plasma (non-reducing conditions)
Lane 2 : Human plasma (reducing conditions)
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate (reducing conditions)
Secondary
All lanes : IRDye800-conjugated Streptavidin
Predicted band size: 69 kDaThe experiment was conducted under reducing and non-reducing conditions.Negative control: HeLa cell lysate.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab81426 has been referenced in 1 publication.
- Edwards W et al. Targeting the ion channel Kv1.3 with scorpion venom peptides engineered for potency, selectivity, and half-life. J Biol Chem 289:22704-14 (2014). ELISA . PubMed: 24939846