Recombinant Biotin Anti-IGLC1 antibody [RM127] (ab224180)
![ELISA - Biotin Anti-IGLC1 antibody [RM127] (ab224180)](https://www.abcam.com/ps/products/224/ab224180/Images/ab224180-284558-ab224180elisa2.jpg "ELISA - Biotin Anti-IGLC1 antibody [RM127] (ab224180)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Biotin Rabbit monoclonal [RM127] to IGLC1
- Suitable for: ELISA, IHC
- Reacts with: Human
- Conjugation: Biotin
Overview
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Product name
Biotin Anti-IGLC1 antibody [RM127]
See all IGLC1 primary antibodies -
Description
Biotin Rabbit monoclonal [RM127] to IGLC1 -
Host species
Rabbit -
Conjugation
Biotin -
Specificity
ab224180 reacts to the lambda light chain of human immunoglobulins. -
Tested applications
Suitable for: ELISA, IHCmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat, Goat -
Immunogen
Full length native protein (purified) corresponding to Human IGLC1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab224180 was purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM127 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224180 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use a concentration of 0.05 - 0.2 µg/ml.
|
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| IHC |
Use a concentration of 0.5 - 2 µg/ml.
|
| Notes |
|---|
|
ELISA
Use a concentration of 0.05 - 0.2 µg/ml. |
|
IHC
Use a concentration of 0.5 - 2 µg/ml. |
Target
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Cellular localization
Extracellular region, plasma membrane. -
Database links
- Entrez Gene: 3537 Human
- Omim: 147220 Human
- SwissProt: P0CG04 Human
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Alternative names
- Ig lambda-1 chain C regions antibody
- IGLC antibody
- immunoglobulin lambda constant 1 (Mcg marker) antibody
see all
Images
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ELISA - Biotin Anti-IGLC1 antibody [RM127] (ab224180)
A titer ELISA using ab224180. The plate was coated with different amounts of human IgG3λ. A serial dilution of ab224180 was used as the primary antibody. An alkaline phosphatase conjugated Anti-Rabbit IgG was used as the secondary antibody.
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![ELISA - Biotin Anti-IGLC1 antibody [RM127] (ab224180)](https://www.abcam.com/ps/products/224/ab224180/Images/ab224180-284557-ab224180elisa1.jpg)
ELISA - Biotin Anti-IGLC1 antibody [RM127] (ab224180)ELISA of human immunoglobulins shows ab224180 reacts to the lambda light chain of human immunoglobulins. No cross reactivity with the kappa light chain, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL or 50 ng/mL of ab224180 was used as the primary antibody. An alkaline phosphatase conjugated Anti-Rabbit IgG was used as the secondary antibody.
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![Immunohistochemistry - Biotin Anti-IGLC1 antibody [RM127] (ab224180)](https://www.abcam.com/ps/products/224/ab224180/Images/ab224180-284556-ab224180IHC.jpg)
Immunohistochemistry - Biotin Anti-IGLC1 antibody [RM127] (ab224180)Human tonsil tissue stained for IGLC1 using ab224180 at 2 µg/ml in immunohistochemical analysis.
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![Biotin Anti-IGLC1 antibody [RM127] (ab224180)](https://www.abcam.com/ps/products/224/ab224180/Images/ab224180-4-benefits-of-recombinant-antibodies.png)
Biotin Anti-IGLC1 antibody [RM127] (ab224180)
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab224180 has not yet been referenced specifically in any publications.