Biotin Anti-Protein A antibody (ab18598)
Key features and details
- Biotin Chicken polyclonal to Protein A
- Suitable for: WB, ELISA
- Reacts with: Staphylococcus aureus
- Conjugation: Biotin
- Isotype: IgY
Overview
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Product name
Biotin Anti-Protein A antibody
See all Protein A primary antibodies -
Description
Biotin Chicken polyclonal to Protein A -
Host species
Chicken -
Conjugation
Biotin -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Staphylococcus aureus -
Immunogen
Recombinant full length protein corresponding to Staphylococcus aureus Protein A.
Database link: P02976 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
After multiple immunizations in Freund’s adjuvant, eggs were collected and delipidated. Antibodies were immunoaffinity purified off protein A agarose and conjugated with biotin. -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab18598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/5000.
|
|
| ELISA |
1/10000 - 1/50000.
|
| Notes |
|---|
|
WB
1/1000 - 1/5000. |
|
ELISA
1/10000 - 1/50000. |
Target
-
Relevance
Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology. -
Cellular localization
Secreted; cell wall. -
Database links
- Entrez Gene: 3919448 Staphylococcus aureus
- SwissProt: B3GGB2 Staphylococcus aureus
- SwissProt: P02976 Staphylococcus aureus
- SwissProt: P38507 Staphylococcus aureus
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Alternative names
- gG-binding protein A antibody
- Immunoglobulin G binding protein A antibody
- SPA antibody
- Staphylococcal protein A antibody
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab18598 has been referenced in 2 publications.
- Abuaita BH et al. Mitochondria-Derived Vesicles Deliver Antimicrobial Reactive Oxygen Species to Control Phagosome-Localized Staphylococcus aureus. Cell Host Microbe 24:625-636.e5 (2018). PubMed: 30449314
- Gilbert FB et al. Differential response of bovine mammary epithelial cells to Staphylococcus aureus or Escherichia coli agonists of the innate immune system. Vet Res 44:40 (2013). WB ; Staphylococcus aureus . PubMed: 23758654