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Biotin Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18445)

Q&A (1)References (3)

Conjugates logo Related conjugates and formulations

FITC Unconjugated

Overview

  • Product name

    Biotin Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control
    See all Rat isotype controls

  • Conjugation

    Biotin

  • Conjugation notes

    ab18445 is conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.

  • Tested applications

    Suitable for: Flow Cytmore details

  • Immunogen

    Trinitrophenol + KLH

  • General notes


    ab18445 RTK2758 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining, bioassay, and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    ab18445 was purified by affinity chromatography,

  • Isotype control notes

    ab18445 RTK2758 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining, bioassay, and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

  • Clonality

    Monoclonal

  • Clone number

    RTK2758

  • Isotype

    IgG2a

  • Light chain type

    kappa

  • Research areas

  • Alternative names

    • Rat Isotype Control

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab18445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use at an assay dependent concentration.

Surface or intracellular staining.
Notes
Flow Cyt
Use at an assay dependent concentration.

Surface or intracellular staining.

References (3)

Publishing research using ab18445? Please let us know so that we can cite the reference in this datasheet.

ab18445 has been referenced in 3 publications.

  • Akiyama S  et al. Th17 cells increase during maturation in peripheral blood of healthy dogs. Vet Immunol Immunopathol 209:17-21 (2019). PubMed: 30885301
  • Zeng SL  et al. Mesenchymal stem cells abrogate experimental asthma by altering dendritic cell function. Mol Med Rep 12:2511-20 (2015). Mouse . PubMed: 25936350
  • Yan F  et al. Down-regulation of extracellular signal-regulated kinase 1/2 activity in P-glycoprotein-mediated multidrug resistant hepatocellular carcinoma cells. World J Gastroenterol 15:1443-51 (2009). Flow Cyt . PubMed: 19322916

Customer reviews and Q&As

Q&A
Submit a question

Question

We are customers of abcam antibody products. We recently purchased ABCAM CN:ab18445 200ug (0.5mg/mL) Rat IgG2a-Biotin LN: GR76161-2

And ABCAM CN:ab37394 200ug (0.5mg/mL) Goat F(ab')2 IgG-Fitc LN: GR41305-1.

On 28Nov12 we had a temperature excursion on our 2-8c Refrigerator which recorded -20c for 4 hrs. We use this product to test all our final products. So we have a Deviation to record and to justify the antibody performance.

Could you please provide any information on the stability of the product after freeze and thaw. I would greatly appreciate of any information.

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Answer

Thank you for contacting us.

We do not have any stability testing data for either of these antibodies. In general, it is recommended to avoid freezing either of these antibodies but it is expected that a single freeze-thaw cycle will not affect the performance of either antibody. Please note that the lot of ab37394 that you have expired in August. (Our records show it was purchased in June 2011). It is possible it may still perform adequately, however. The lot of ab18445 that you have, GR76161-2, expires in May 2013.

Please contact us if you have any more questions.

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