Biotin Anti-S100A4 antibody [S100A4/1481] (ab271820)
Key features and details
- Biotin Mouse monoclonal [S100A4/1481] to S100A4
- Suitable for: IHC-P, Protein Array
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG1
Related conjugates and formulations
Overview
-
Product name
Biotin Anti-S100A4 antibody [S100A4/1481]
See all S100A4 primary antibodies -
Description
Biotin Mouse monoclonal [S100A4/1481] to S100A4 -
Host species
Mouse -
Conjugation
Biotin -
Tested applications
Suitable for: IHC-P, Protein Arraymore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Mouse S100A4 aa 1-200. The exact sequence is proprietary.
Database link: P07091 -
Positive control
- IHC-P: Human placenta tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
-
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
S100A4/1481 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
-
Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
Protein Array |
Use at an assay dependent concentration.
|
Notes |
---|
IHC-P
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Protein Array
Use at an assay dependent concentration. |
Target
-
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the S-100 family.
Contains 2 EF-hand domains. - Information by UniProt
-
Database links
- Entrez Gene: 6275 Human
- Omim: 114210 Human
- SwissProt: P26447 Human
- Unigene: 654444 Human
-
Alternative names
- 18A2 antibody
- 42A antibody
- calcium Placental protein antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-S100A4 antibody [S100A4/1481] (ab271820)
Formalin-fixed, paraffin-embedded human Placenta tissue stained for S100A4 using ab271820 at 4 µg/ml in immunohistochemical analysis
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-S100A4 antibody [S100A4/1481] (ab271820)
Formalin-fixed, paraffin-embedded human Placenta tissue stained for S100A4 using ab271820 at 4 µg/ml in immunohistochemical analysis
-
Analysis of Protein Array containing more than 19,000 full-length human proteins using ab271820. Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (0)
ab271820 has not yet been referenced specifically in any publications.