Biotin Anti-TLR9 antibody [5G5] (ab17236)
Key features and details
- Biotin Mouse monoclonal [5G5] to TLR9
- Suitable for: IHC-Fr, IHC-P, ICC/IF, Flow Cyt, WB
- Reacts with: Mouse, Dog, Human
- Conjugation: Biotin
- Isotype: IgG2a
Overview
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Product name
Biotin Anti-TLR9 antibody [5G5]
See all TLR9 primary antibodies -
Description
Biotin Mouse monoclonal [5G5] to TLR9 -
Host species
Mouse -
Conjugation
Biotin -
Tested applications
Suitable for: IHC-Fr, IHC-P, ICC/IF, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Dog, Human -
Immunogen
Recombinant fragment within TLR9. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- RAW macrophages and TLR9 transfected HEK293 cells. RAW264.7 macrophages stimulated with IFN gamma.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
Purification notes
0.2 µm filtered -
Clonality
Monoclonal -
Clone number
5G5 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab17236 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent concentration.
ab97679 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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| WB | (1) |
Use at an assay dependent concentration.
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| Notes |
|---|
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IHC-Fr
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. ab97679 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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WB
Use at an assay dependent concentration. |
Target
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Function
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
Tissue specificity
Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver. -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 26 LRR (leucine-rich) repeats.
Contains 1 TIR domain. -
Cellular localization
Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist. - Information by UniProt
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Database links
- Entrez Gene: 54106 Human
- Entrez Gene: 81897 Mouse
- Omim: 605474 Human
- SwissProt: Q9NR96 Human
- SwissProt: Q9EQU3 Mouse
- Unigene: 87968 Human
- Unigene: 44889 Mouse
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Alternative names
- CD 289 antibody
- CD289 antibody
- TLR 9 antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (5)
ab17236 has been referenced in 5 publications.
- Wang CY et al. TLR9 Binding to Beclin 1 and Mitochondrial SIRT3 by a Sodium-Glucose Co-Transporter 2 Inhibitor Protects the Heart from Doxorubicin Toxicity. Biology (Basel) 9:N/A (2020). PubMed: 33138323
- Liu FY et al. TLR9 is essential for HMGB1-mediated post-myocardial infarction tissue repair through affecting apoptosis, cardiac healing, and angiogenesis. Cell Death Dis 10:480 (2019). PubMed: 31209243
- Chen GY et al. Simultaneous induction of autophagy and toll-like receptor signaling pathways by graphene oxide. Biomaterials 33:6559-69 (2012). ICC/IF, Flow Cyt ; Mouse . PubMed: 22704844
- Chen GY et al. Baculovirus transduction of mesenchymal stem cells triggers the toll-like receptor 3 pathway. J Virol 83:10548-56 (2009). Flow Cyt ; Human . PubMed: 19656899
- Tomchuck SL et al. Toll-like receptors on human mesenchymal stem cells drive their migration and immunomodulating responses. Stem Cells 26:99-107 (2008). Flow Cyt ; Human . PubMed: 17916800
