Anti-BirA antibody [6C4c7] (ab232732)
![Immunocytochemistry/ Immunofluorescence - Anti-BirA antibody [6C4c7] (ab232732)](https://www.abcam.com/ps/products/232/ab232732/Images/ab232732-381164-antibira6c4c7icchek293transfecteddox.jpg "Immunocytochemistry/ Immunofluorescence - Anti-BirA antibody [6C4c7] (ab232732)")
Key features and details
- Mouse monoclonal [6C4c7] to BirA
- Suitable for: ICC/IF, WB
- Reacts with: Escherichia coli
- Isotype: IgG1
Overview
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Product name
Anti-BirA antibody [6C4c7]
See all BirA primary antibodies -
Description
Mouse monoclonal [6C4c7] to BirA -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Escherichia coli -
Immunogen
Recombinant fragment (GST-tag) within Escherichia coli BirA aa 1-150 (N terminal). The exact sequence is proprietary. Glutathione-S-transferase fused to E.coli BirA R118G
Database link: P06709 -
Positive control
- WB: BirA-transfected HEK293T whole cell lysates (induced). ICC: BirA transfected HEK293T (induced) cells
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
6C4c7 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232732 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 5 µg/ml.
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| WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa.
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| Notes |
|---|
|
ICC/IF
Use a concentration of 5 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. |
Target
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Relevance
BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins. -
Database links
- Entrez Gene: 4491453 Escherichia coli
- SwissProt: P06709 Escherichia coli
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Alternative names
- Bifunctional protein birA antibody
- Bio R antibody
- BioR antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-BirA antibody [6C4c7] (ab232732)
ab232732 staining BirA in normal HEK293 cells, BirA transfected HEK293T cells (uninduced) and BirA transfected HEK293T cells induced with DOX. The cells were fixed with 100% MeOH (5min), permeabilized with 0.1%PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab232732 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Cell samples kindly provided by the Brian Burke laboratory, A-Star Institute, Singapore.
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![Western blot - Anti-BirA antibody [6C4c7] (ab232732)](https://www.abcam.com/ps/products/232/ab232732/Images/ab232732-353191-ab232732AP47773435pcMilkPublish.jpg)
Western blot - Anti-BirA antibody [6C4c7] (ab232732)All lanes : Anti-BirA antibody [6C4c7] (ab232732) at 1 µg/ml
Lane 1 : HEK293 whole cell lysate
Lane 2 : HEK293T whole cell lysate
Lane 3 : BirA-SLAP75 transfected HEK293T whole cell lysate, mock induced
Lane 4 : BirA-SLAP75 transfected HEK293T whole cell lysate, DOX induced
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?This blot was produced using a 4-12% Bis-tris under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour using 5% milk before ab232732 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 1µg/ml concentration and 1/20,000 dilution respectively. Antibody binding was detected using Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
Cell samples kindly provided by the Brian Burke laboratory, A-Star Institute, Singapore.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab232732 has been referenced in 1 publication.
- Droujinine IA et al. Proteomics of protein trafficking by in vivo tissue-specific labeling. Nat Commun 12:2382 (2021). PubMed: 33888706