Anti-BMP4 antibody (ab39973)
Key features and details
- Rabbit polyclonal to BMP4
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-BMP4 antibody
See all BMP4 primary antibodies -
Description
Rabbit polyclonal to BMP4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Pig
-
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human BMP4.
Read Abcam's proprietary immunogen policy (Peptide available as ab40140.) -
Positive control
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab39973 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (5) |
Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
|
| ICC/IF | (1) |
Use a concentration of 1 µg/ml.
|
| Notes |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). |
|
ICC/IF
Use a concentration of 1 µg/ml. |
Target
-
Function
Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction. -
Tissue specificity
Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines. -
Involvement in disease
Defects in BMP4 are the cause of microphthalmia syndromic type 6 (MCOPS6) [MIM:607932]; also known as microphthalmia and pituitary anomalies or microphthalmia with brain and digit developmental anomalies. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS6 is characterized by microphthalmia/anophthalmia associated with facial, genital, skeletal, neurologic and endocrine anomalies.
Defects in BMP4 are the cause of non-syndromic orofacial cleft type 11 (OFC11) [MIM:600625]. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. OFC11 is an unusual anomaly consisting of a paramedian scar of the upper lip with an appearance suggesting that a typical cleft lip was corrected in utero. -
Sequence similarities
Belongs to the TGF-beta family. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
-
Database links
- Entrez Gene: 652 Human
- Entrez Gene: 12159 Mouse
- Entrez Gene: 25296 Rat
- Omim: 112262 Human
- SwissProt: P12644 Human
- SwissProt: P21275 Mouse
- SwissProt: Q06826 Rat
- Unigene: 68879 Human
see all -
Alternative names
- zgc:100779 antibody
- BMP 2B antibody
- BMP 4 antibody
see all
Images
-
Western blot - Anti-BMP4 antibody (ab39973)All lanes : Anti-BMP4 antibody (ab39973) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-BMP4 antibody (ab39973)This image is courtesy of Ruma Raha-Chowdhury, University Of Cambridge, United KingdomICC/IF image of ab39973 staining primary Rat hippocampal neurons. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 5% normal donkey serum / 0.3M glycine in 0.1% PBS-Tritonx100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39973, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488donkey anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
-
Western blot - Anti-BMP4 antibody (ab39973)All lanes : Anti-BMP4 antibody (ab39973) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Mouse Tissue Lysate
Lane 3 : Human liver tissue lysate - total protein (ab29889)
Lane 4 : Human heart tissue lysate - total protein (ab29431)
Lane 5 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 6 : Human brain tissue lysate - total protein (ab29466) with Human BMP4 peptide (ab40140) at 1 µg/ml
Lane 7 : Brain (Mouse) Mouse Tissue Lysate with Human BMP4 peptide (ab40140) at 1 µg/ml
Lane 8 : Human liver tissue lysate - total protein (ab29889) with Human BMP4 peptide (ab40140) at 1 µg/ml
Lane 9 : Human heart tissue lysate - total protein (ab29431) with Human BMP4 peptide (ab40140) at 1 µg/ml
Lane 10 : Human skeletal muscle tissue lysate - total protein (ab29330) with Human BMP4 peptide (ab40140) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 47 kDa
ab39973 detects a 47kDa band that we believe corresponds to the pre-cleaved form of BMP4 with the immunizing peptide raised against a region located in the propeptide. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
Western blot - Anti-BMP4 antibody (ab39973)This image is courtesy of Ruma Raha-Chowdhury, University Of Cambridge, United KingdomAll lanes : Anti-BMP4 antibody (ab39973) at 1/1000 dilution
All lanes : mouse brain
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Dako Cytomation P0260
Conjugation: HRP at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Exposure time: 1 minute
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (77)
ab39973 has been referenced in 77 publications.
- Shido R et al. Gene-activated matrix harboring a miR20a-expressing plasmid promotes rat cranial bone augmentation. Regen Biomater 8:rbaa060 (2021). PubMed: 33738113
- Tan L et al. Overexpression of Long Non-coding RNA 4933425B07Rik Causes Urinary Malformations in Mice. Front Cell Dev Biol 9:594640 (2021). PubMed: 33681192
- Li H et al. Hypermethylation-mediated downregulation of long non-coding RNA MEG3 inhibits osteogenic differentiation of bone marrow mesenchymal stem cells and promotes pediatric aplastic anemia. Int Immunopharmacol 93:107292 (2021). PubMed: 33529912
- Zhang X et al. BMP4 overexpression induces the upregulation of APP/Tau and memory deficits in Alzheimer's disease. Cell Death Discov 7:51 (2021). PubMed: 33723239
- Zhang J et al. Augmented BMP4 signal impairs tongue myogenesis. J Mol Histol 52:651-659 (2021). PubMed: 34076834