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RecombinantRabMAb

Recombinant Anti-BMP4 antibody [EPR6211] (ab124715)

Submit a review Submit a question References (17)
Western blot - Anti-BMP4 antibody [EPR6211] (ab124715)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody [EPR6211] (ab124715)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody [EPR6211] (ab124715)
  • Immunoprecipitation - Anti-BMP4 antibody [EPR6211] (ab124715)
  • Flow Cytometry (Intracellular) - Anti-BMP4 antibody [EPR6211] (ab124715)
  • Immunocytochemistry/ Immunofluorescence - Anti-BMP4 antibody [EPR6211] (ab124715)
  • Western blot - Anti-BMP4 antibody [EPR6211] (ab124715)
  • OI-RD Scanning - Anti-BMP4 antibody [EPR6211] (ab124715)
  • Anti-BMP4 antibody [EPR6211] (ab124715)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6211] to BMP4
  • Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 Biotin Carrier Free HRP

Overview

  • Product name

    Anti-BMP4 antibody [EPR6211]
    See all BMP4 primary antibodies

  • Description

    Rabbit monoclonal [EPR6211] to BMP4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to Human BMP4 aa 350 to the C-terminus.

  • Positive control

    • WB: Human fetal liver, A549, HeLa, A431, and Human heart lysates IP: A549 whole cell lysate. IHC-P: Human breast cancer, and colon Tissue Flow Cyt (intra): HeLa cells ICC/IF: A549 cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Predicted molecular weight: 47 kDa.
IP
1/10 - 1/100.
IHC-P
1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified use at 1/100 - 1/250. See IHC antigen retrieval protocols.
ICC/IF
1/500.

For unpurified use at 1/50 - 1/100. 
Notes
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Predicted molecular weight: 47 kDa.
IP
1/10 - 1/100.
IHC-P
1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified use at 1/100 - 1/250. See IHC antigen retrieval protocols.
ICC/IF
1/500.

For unpurified use at 1/50 - 1/100. 

Target

  • Function

    Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction.

  • Tissue specificity

    Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines.

  • Involvement in disease

    Defects in BMP4 are the cause of microphthalmia syndromic type 6 (MCOPS6) [MIM:607932]; also known as microphthalmia and pituitary anomalies or microphthalmia with brain and digit developmental anomalies. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS6 is characterized by microphthalmia/anophthalmia associated with facial, genital, skeletal, neurologic and endocrine anomalies.
    Defects in BMP4 are the cause of non-syndromic orofacial cleft type 11 (OFC11) [MIM:600625]. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. OFC11 is an unusual anomaly consisting of a paramedian scar of the upper lip with an appearance suggesting that a typical cleft lip was corrected in utero.

  • Sequence similarities

    Belongs to the TGF-beta family.

  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

  • Database links

    • Alternative names

      • zgc:100779 antibody
      • BMP 2B antibody
      • BMP 4 antibody
      • BMP-2B antibody
      • BMP-4 antibody
      • BMP2B antibody
      • BMP2B1 antibody
      • BMP4 antibody
      • BMP4_HUMAN antibody
      • Bone morphogenetic protein 2B antibody
      • Bone morphogenetic protein 4 antibody
      • DVR4 antibody
      • MCOPS6 antibody
      • MGC100779 antibody
      • OFC11 antibody
      • zbmp-4 antibody
      • ZYME antibody
      see all

    Images

    • Western blot - Anti-BMP4 antibody [EPR6211] (ab124715)
      Western blot - Anti-BMP4 antibody [EPR6211] (ab124715)
      Anti-BMP4 antibody [EPR6211] (ab124715) at 0.05 µg/ml (purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 47 kDa



      Blocking and diluting buffer: 5% NFDM/TBST
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody [EPR6211] (ab124715)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody [EPR6211] (ab124715)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast cancer tissue sections labeling BMP4 with Purified ab124715 at 1:400 dilution (2.44 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody [EPR6211] (ab124715)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody [EPR6211] (ab124715)

      Unpurified ab124715, at 1/100, staining BMP4 in paraffin embedded Human colon tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-BMP4 antibody [EPR6211] (ab124715)
      Immunoprecipitation - Anti-BMP4 antibody [EPR6211] (ab124715)

      ab124715 (purified) at 1:60 dilution (2µg) immunoprecipitating BMP4 in A549 whole cell lysate.
      Lane 1 (input): A549 (Human lung carcinoma epithelial cell) whole cell lysate 10µg
      Lane 2 (+): ab124715 & A549 whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124715 in A549 whole cell lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • Flow Cytometry (Intracellular) - Anti-BMP4 antibody [EPR6211] (ab124715)
      Flow Cytometry (Intracellular) - Anti-BMP4 antibody [EPR6211] (ab124715)

      Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling BMP4 with purified ab124715 at 1/120 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

       

    • Immunocytochemistry/ Immunofluorescence - Anti-BMP4 antibody [EPR6211] (ab124715)
      Immunocytochemistry/ Immunofluorescence - Anti-BMP4 antibody [EPR6211] (ab124715)

      Immunocytochemistry/Immunofluorescence analysis of A549 (human lung carcinoma) cells labelling BMP4 with purified ab124715 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

      Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

    • Western blot - Anti-BMP4 antibody [EPR6211] (ab124715)
      Western blot - Anti-BMP4 antibody [EPR6211] (ab124715)
      All lanes : Anti-BMP4 antibody [EPR6211] (ab124715) at 1/10000 dilution (Unpurified)

      Lane 1 : Human fetal liver lysate
      Lane 2 : A549 lysate
      Lane 3 : HeLa lysate
      Lane 4 : A431 lysate
      Lane 5 : Human heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 47 kDa

    • OI-RD Scanning - Anti-BMP4 antibody [EPR6211] (ab124715)
      OI-RD Scanning - Anti-BMP4 antibody [EPR6211] (ab124715)

      Produced using unpurified ab124715

      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    • Anti-BMP4 antibody [EPR6211] (ab124715)
      Anti-BMP4 antibody [EPR6211] (ab124715)

    References (17)

    Publishing research using ab124715? Please let us know so that we can cite the reference in this datasheet.

    ab124715 has been referenced in 17 publications.

    • Ding R  et al. Antrodia camphorata extract (ACE)-induced apoptosis is associated with BMP4 expression and p53-dependent ROS generation in human colon cancer cells. J Ethnopharmacol 268:113570 (2021). PubMed: 33181285
    • Zhang X  et al. Exploring the five different genes associated with PKCa in bladder cancer based on gene expression microarray. J Cell Mol Med 25:1759-1770 (2021). PubMed: 33452764
    • Chen H  et al. CHRDL2 promotes osteosarcoma cell proliferation and metastasis through the BMP-9/PI3K/AKT pathway. Cell Biol Int 45:623-632 (2021). PubMed: 33245175
    • Liu L  et al. A high level of lncFGD5-AS1 inhibits epithelial-to-Mesenchymal transition by regulating the miR-196a-5p/SMAD6/BMP axis in gastric Cancer. BMC Cancer 21:453 (2021). PubMed: 33892661
    • Li M  et al. GAS5 protects against osteoporosis by targeting UPF1/SMAD7 axis in osteoblast differentiation. Elife 9:N/A (2020). PubMed: 33006314
    View all Publications for this product

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