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RecombinantRabMAb

Recombinant Anti-BMP7 antibody [EPR5897] (ab129156)

Reviews (1)Q&A (2)References (5)
Western blot - Anti-BMP7 antibody [EPR5897] (ab129156)
  • Western blot - Anti-BMP7 antibody [EPR5897] (ab129156)
  • Immunoprecipitation - Anti-BMP7 antibody [EPR5897] (ab129156)
  • Western blot - Anti-BMP7 antibody [EPR5897] (ab129156)
  • Western blot - Anti-BMP7 antibody [EPR5897] (ab129156)
  • Anti-BMP7 antibody [EPR5897] (ab129156)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5897] to BMP7
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-BMP7 antibody [EPR5897]
    See all BMP7 primary antibodies

  • Description

    Rabbit monoclonal [EPR5897] to BMP7

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human BMP7 aa 300-400. The exact sequence is proprietary.

  • Positive control

    • WB: Rat and mouse kidney lysate, Fetal kidney, Human prostate and HT-1376 lysates; recombinant Osteogenic Growth Peptide IP: MCF7 whole cell lysate

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab129156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 49 kDa.
IP
1/150.

For unpurified use at 1/10 - 1/100.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 49 kDa.
IP
1/150.

For unpurified use at 1/10 - 1/100.
Application notes
Is unsuitable for ICC/IF or IHC-P.

Target

  • Function

    Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.

  • Tissue specificity

    Expressed in the kidney and bladder. Lower levels seen in the brain.

  • Sequence similarities

    Belongs to the TGF-beta family.

  • Developmental stage

    Expressed in the developing eye, brain and ear during embryogenesis.

  • Post-translational
    modifications

    Several N-termini starting at positions 293, 300, 315 and 316 have been identified by direct sequencing resulting in secretion of different mature forms (PubMed:17977014).

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • BMP 7 antibody
      • BMP-7 antibody
      • Bmp7 antibody
      • BMP7_HUMAN antibody
      • Bone morphogenetic protein 7 antibody
      • Bone morphogenic protein 7 antibody
      • Eptotermin alfa antibody
      • OP 1 antibody
      • OP-1 antibody
      • OP1 antibody
      • Osteogenic protein 1 antibody
      see all

    Images

    • Western blot - Anti-BMP7 antibody [EPR5897] (ab129156)
      Western blot - Anti-BMP7 antibody [EPR5897] (ab129156)
      All lanes : Anti-BMP7 antibody [EPR5897] (ab129156) at 1/5000 dilution (purified)

      Lane 1 : Rat kidney lysate
      Lane 2 : Mouse kidney lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size: 49 kDa
      Observed band size: 49 kDa



      Blocking/Diluting buffer 5% NFDM/TBST
    • Western blot - Anti-BMP7 antibody [EPR5897] (ab129156)
      Western blot - Anti-BMP7 antibody [EPR5897] (ab129156)
      Anti-BMP7 antibody [EPR5897] (ab129156) at 1/1000 dilution (purified) + MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 1/15 dilution

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 49 kDa
      Observed band size: 49 kDa



      Blocking/Diluting buffer 5% NFDM/TBST
    • Immunoprecipitation - Anti-BMP7 antibody [EPR5897] (ab129156)
      Immunoprecipitation - Anti-BMP7 antibody [EPR5897] (ab129156)

      Purified ab129156 at 1/150 immunoprecipitating BMP7 in MCF7 (Human breast adenocarcinoma cell line) whole cell lysate observed at 49 KDa (lanes 1 and 2).

      Lane 1 (input): MCF7 whole cell lysate 10μg

      Lane 2 (+): ab129156 + MCF7 whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab129156 in MCF7 whole cell lysate

      For western blotting, ab129156 at 1/150 dilution and VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Blocking and Diluting buffer and concentration: 5% NFDM/TBST.

    • Western blot - Anti-BMP7 antibody [EPR5897] (ab129156)
      Western blot - Anti-BMP7 antibody [EPR5897] (ab129156)
      All lanes : Anti-BMP7 antibody [EPR5897] (ab129156) at 1/1000 dilution (unpurified)

      Lane 1 : Fetal kidney lysate
      Lane 2 : Human prostate lysate
      Lane 3 : HT-1376 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 49 kDa

    • Western blot - Anti-BMP7 antibody [EPR5897] (ab129156)
      Western blot - Anti-BMP7 antibody [EPR5897] (ab129156)
      Anti-BMP7 antibody [EPR5897] (ab129156) at 1/1000 dilution (unpurified) + recombinant Human BMP7 (amino acids 316 - 431) at 0.01 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 49 kDa



      The recombinant peptide should give a band of approximately 13.7 kDa.
    • Anti-BMP7 antibody [EPR5897] (ab129156)
      Anti-BMP7 antibody [EPR5897] (ab129156)

    References (5)

    Publishing research using ab129156? Please let us know so that we can cite the reference in this datasheet.

    ab129156 has been referenced in 5 publications.

    • Zhu H  et al. Glucocorticoid counteracts cellular mechanoresponses by LINC01569-dependent glucocorticoid receptor-mediated mRNA decay. Sci Adv 7:N/A (2021). PubMed: 33627425
    • Li M  et al. GAS5 protects against osteoporosis by targeting UPF1/SMAD7 axis in osteoblast differentiation. Elife 9:N/A (2020). PubMed: 33006314
    • Tang S  et al. LncRNA-OG Promotes the Osteogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells Under the Regulation of hnRNPK. Stem Cells 37:270-283 (2019). PubMed: 30372559
    • Lettlova S  et al. MiR-301a-3p Suppresses Estrogen Signaling by Directly Inhibiting ESR1 in ERa Positive Breast Cancer. Cell Physiol Biochem 46:2601-2615 (2018). PubMed: 29763890
    • Yao H  et al. BMP7 antagonizes proliferative vitreoretinopathy through retinal pigment epithelial fibrosis in vivo and in vitro. FASEB J N/A:fj201800858RR (2018). PubMed: 30383450

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-BMP7 antibody [EPR5897]

     Inconclusive
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (brain)
    Permeabilization
    Yes - 0.3% TritonX-100
    Specification
    brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 27 2015

    Question

    The customer has now asked:

    Are you aware whether it will or can interfere in any which way? I am going to Immunoprecipitate human chondrocytes against this antibody. I do not want any molecule of the OP-1 produced by my cells to go undetected due to some resistance issue.

    As far as I can tell the concern is that he wants to ensure that all of the OP-1 in his system will be immunoprecipitated by the antibody. Any thoughts?

    Read More
    Answer

    We unfortunately do not have data that would answer your customer's question about whether the antibody has more affinity for the BMP7 precursor or mature form. What is needed is a comparison of the recovery of each protein form (precursor and mature) in the IP product and in the flow-through fraction of proteins that are not precipitated.

    Read More

    Question

    1) As per the data sheet there is an image showing both precursor and the mature form of human BMP7….is it specific for both forms or more for mature? Does the antibody bind both forms with equal affinity, so that the signal strength on the Western is dependent on the amount of each form in the sample?
    2) I will be using human chondrocytres isolated from cartilage for IP work. Do you have any references for cartilage?

    Read More
    Answer

    The BMP7 antibody ab129156 is raised against an amino acid sequence that is present in the precursor as well as the mature protein. For western blotting, the antibody should be able to detect both forms with equal affinity, if the samples are denatured and reduced. If experimental samples contain native, non-denatured protein, the antibody may have higher affinity for one or the other, depending on their native conformation, which will determine how available the amino acid sequence is for antibody recognition. We do not have data demonstrating this, though.

    We are not aware of any references that have used this antibody for IP of cartilage, or for any assay of BMP7 in cartilage or chondrocytes.

    Read More

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