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RecombinantRabMAb

Recombinant Anti-BOG antibody [EPR9905] (ab157202)

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Western blot - Anti-BOG antibody [EPR9905] (ab157202)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOG antibody [EPR9905] (ab157202)
  • Immunocytochemistry/ Immunofluorescence - Anti-BOG antibody [EPR9905] (ab157202)
  • Flow Cytometry (Intracellular) - Anti-BOG antibody [EPR9905] (ab157202)
  • Anti-BOG antibody [EPR9905] (ab157202)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9905] to BOG
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-BOG antibody [EPR9905]
    See all BOG primary antibodies

  • Description

    Rabbit monoclonal [EPR9905] to BOG

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide within Human BOG. The exact sequence is proprietary.
    Database link: O75884

  • Positive control

    • HepG2, MCF7, and HeLa cell lysates; kidney tissue; MCF7 and HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    pH: 7.2
    Constituents: 0.35% Sodium citrate, 0.17% Sodium chloride, 0.03% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR9905

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab157202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
Notes
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
Application notes
Is unsuitable for IP.

Target

  • Function

    May play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-beta1 through interaction with retinoblastoma and the subsequent displacement of E2F-1.

  • Tissue specificity

    Widely expressed. Expressed at higher levels in tumor tissues than in normal tissues.

  • Sequence similarities

    Belongs to the RBBP9 family.

  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • B5T overexpressed gene protein antibody
      • B5T-overexpressed gene protein antibody
      • BOG antibody
      • Bog protein antibody
      • MGC9236 antibody
      • OTTHUMP00000030360 antibody
      • Protein BOG antibody
      • Putative hydrolase RBBP9 antibody
      • RBBP 10 antibody
      • RBBP 9 antibody
      • RBBP-10 antibody
      • RBBP-9 antibody
      • RBBP10 antibody
      • RBBP9 antibody
      • RBBP9_HUMAN antibody
      • Retinoblastoma binding protein 10 antibody
      • Retinoblastoma binding protein 9 antibody
      • Retinoblastoma-binding protein 10 antibody
      • Retinoblastoma-binding protein 9 antibody
      • Retinoma binding protein 9 antibody
      see all

    Images

    • Western blot - Anti-BOG antibody [EPR9905] (ab157202)
      Western blot - Anti-BOG antibody [EPR9905] (ab157202)
      All lanes : Anti-BOG antibody [EPR9905] (ab157202) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : MCF-7 cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 21 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOG antibody [EPR9905] (ab157202)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOG antibody [EPR9905] (ab157202)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling BOG using ab157202 at a 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-BOG antibody [EPR9905] (ab157202)
      Immunocytochemistry/ Immunofluorescence - Anti-BOG antibody [EPR9905] (ab157202)

      Immunofluorescence analysis of MCF7 cells, labeling BOG using ab157202 at a 1/250 dilution.

    • Flow Cytometry (Intracellular) - Anti-BOG antibody [EPR9905] (ab157202)
      Flow Cytometry (Intracellular) - Anti-BOG antibody [EPR9905] (ab157202)

      Intracellular Flow Cytometry analysis of permeabilized HeLa cells labeling BOG (red), using ab157202 at a 1/100 dilution, and negative control cells probed with a Rabbit IgG (green) 

       

       

    • Anti-BOG antibody [EPR9905] (ab157202)
      Anti-BOG antibody [EPR9905] (ab157202)

    References (0)

    Publishing research using ab157202? Please let us know so that we can cite the reference in this datasheet.

    ab157202 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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