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  1. Link

    products/primary-antibodies/bpifb1-antibody-ab183143.pdf

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Immunology Secreted Molecules Other secreted molecules
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Anti-BPIFB1 antibody (ab183143)

  • Datasheet
  • SDS
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Western blot - Anti-BPIFB1 antibody (ab183143)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BPIFB1 antibody (ab183143)

Key features and details

  • Rabbit polyclonal to BPIFB1
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
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Recombinant Human BPIFB1 protein (His tag) (ab276525)

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Overview

  • Product name

    Anti-BPIFB1 antibody
    See all BPIFB1 primary antibodies
  • Description

    Rabbit polyclonal to BPIFB1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human BPIFB1 aa 100-200. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_149974).
    Database link: Q8TDL5

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human tongue and laryngocarcinoma tissue lysates; Human breast cancer tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.05% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine)

    Note: PBS without Mg2+ and Ca2+.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Secreted Molecules
    • Other secreted molecules
    • Microbiology
    • Interspecies Interaction
    • Host Immune Response

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab183143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/2000. Predicted molecular weight: 52 kDa.
IHC-P
1/25 - 1/100.
Notes
WB
1/500 - 1/2000. Predicted molecular weight: 52 kDa.
IHC-P
1/25 - 1/100.

Target

  • Function

    May play a role in innate immunity in mouth, nose and lungs.
  • Tissue specificity

    Detected in trachea, nasal septal epithelium and lung.
  • Sequence similarities

    Belongs to the BPI/LBP/Plunc superfamily. Plunc family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession Q8TDL5 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 92747 Human
    • SwissProt: Q8TDL5 Human
    • Unigene: 65551 Human
    • Alternative names

      • BPI fold containing family B, member 1 antibody
      • C20orf114 antibody
      • Long palate antibody
      • Long palate, lung and nasal epithelium carcinoma associated 1 antibody
      • Long palate, lung and nasal epithelium carcinoma associated protein 1 antibody
      • LPLC1_HUMAN antibody
      • Lplunc1 antibody
      • lung and nasal epithelium carcinoma-associated protein 1 antibody
      • VEMSGP antibody
      • Von Ebner minor salivary gland protein antibody
      see all

    Images

    • Western blot - Anti-BPIFB1 antibody (ab183143)
      Western blot - Anti-BPIFB1 antibody (ab183143)
      All lanes : Anti-BPIFB1 antibody (ab183143) at 1/500 dilution

      Lane 1 : Human tongue tissue lysate
      Lane 2 : Human laryngocarcinoma tissue lysate

      Lysates/proteins at 50 µg per lane.

      Secondary
      All lanes : Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution

      Developed using the ECL technique.

      Predicted band size: 52 kDa


      Exposure time: 1 minute


      Gel: 10% SDS-PAGE

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BPIFB1 antibody (ab183143)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BPIFB1 antibody (ab183143)

      Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling BPIFB1 with ab183143 at 1/25 dilution, in the presence (right panel) or absence (left panel) of immunizing peptide.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab183143? Please let us know so that we can cite the reference in this datasheet.

    ab183143 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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