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Anti-BPTF / FALZ antibody [2343C3a] (ab54356)

Reviews (2)Q&A (2)
Western blot - Anti-BPTF / FALZ antibody [2343C3a] (ab54356)

    Key features and details

    • Mouse monoclonal [2343C3a] to BPTF / FALZ
    • Suitable for: WB
    • Reacts with: Recombinant fragment
    • Isotype: IgG1

    Overview

    • Product name

      Anti-BPTF / FALZ antibody [2343C3a]
      See all BPTF / FALZ primary antibodies

    • Description

      Mouse monoclonal [2343C3a] to BPTF / FALZ

    • Host species

      Mouse

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Recombinant fragment

    • Immunogen

      Recombinant fragment (human) from an internal sequence.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab54356 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (1)
    Use at an assay dependent concentration. Predicted molecular weight: 311 kDa.
    Notes
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 311 kDa.

    Target

    • Function

      Histone-binding component of NURF (nucleosome-remodeling factor), a complex which catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin. Specifically recognizes H3 tails trimethylated on 'Lys-4' (H3K4me3), which mark transcription start sites of virtually all active genes. May also regulate transcription through direct binding to DNA or transcription factors.

    • Tissue specificity

      Ubiquitously expressed, with highest levels in testis. Present in kidney, liver and brain. In the brain, highest levels are found in motor cortex (at protein level).

    • Sequence similarities

      Belongs to the PBTF family.
      Contains 1 bromo domain.
      Contains 1 DDT domain.
      Contains 2 PHD-type zinc fingers.

    • Developmental stage

      Abundantly expressed in the fetal brain. Present throughout the gray and white matter of the developing spinal cord at 18-22 gestational weeks. Expressed at low levels in adult brain and spinal cord and reexpressed in neurodegenerative diseases (at protein level).

    • Domain

      The second PHD-type zinc finger mediates binding to histone H3K4Me3. Has specificity for trimethyllysine; introducing a mutation in the Tyr-2876 residue can induce binding to dimethyllysine.

    • Post-translational
      modifications

      Phosphorylation enhances DNA-binding.
      Highly susceptible to proteolysis.

    • Cellular localization

      Cytoplasm. Nucleus. In brains of Alzheimer disease patients, present in a subset of amyloid-containing plaques.
    • Information by UniProt

    • Alternative names

      • BPTF antibody
      • BPTF_HUMAN antibody
      • Bromodomain and PHD finger containing transcription factor antibody
      • Bromodomain and PHD finger-containing transcription factor antibody
      • Bromodomain PHD finger transcription factor antibody
      • FAC 1 antibody
      • fac1 antibody
      • FALZ antibody
      • Fetal Alz 50 clone 1 protein antibody
      • Fetal Alz 50 reactive clone 1 antibody
      • Fetal Alz-50 clone 1 protein antibody
      • Fetal Alzheimer antigen antibody
      • Nucleosome remodeling factor antibody
      • Nucleosome remodeling factor subunit BPTF antibody
      • Nucleosome remodeling factor, large subunit antibody
      • Nucleosome-remodeling factor subunit BPTF antibody
      • NURF 301 antibody
      • NURF301 antibody
      see all

    Images

    • Western blot - Anti-BPTF / FALZ antibody [2343C3a] (ab54356)
      Western blot - Anti-BPTF / FALZ antibody [2343C3a] (ab54356)
      Anti-BPTF / FALZ antibody [2343C3a] (ab54356) + immunising recombinant protein fragment.

      Predicted band size: 311 kDa

    References (0)

    Publishing research using ab54356? Please let us know so that we can cite the reference in this datasheet.

    ab54356 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-BPTF / FALZ antibody [2343C3a]

     Poor
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U2-OS cells)
    Loading amount
    50 µg
    Specification
    U2-OS cells
    Gel Running Conditions
    Non-reduced Denaturing (6)
    Blocking step
    Milk as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Ms. Ayelet Star

    Verified customer

    Submitted Feb 22 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-BPTF / FALZ antibody [2343C3a]

     Poor
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U2-OS cells)
    Specification
    U2-OS cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Triton 0.5%
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 22 2013

    Question

    The customer is planning to check the BPTF antibody for immunoflourecence staining in human cell lines (such as Hela or U2-OS cells).
    She wants to test it's specificity by also staining BPTF depleted cells.

    Thank you for the reply.

    Warm Regards,

    Read More
    Answer

    Thank you very much for getting back to me and for confirming the sample type. It is much appreciated.
    DISCOUNT CODE: 40403
    Expiration date: 04/04/2013
    Value: £227.00
    I am very pleased to hear you would like to accept our offer and test ab54356 in Human ICC/IF. This code will give you £227.00 off your next order before the expiration date. To redeem this offer, please submit an Abreview for Human ICC/IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
    The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

    Read More

    Question

    Dear Tech Support Team,

    Our customer is willing to test ab54356 in ICC/IF with human samples. Are you interested in this type of the information for the AbTrial program? (The customer buys certain antibodies and upload Abreview on Abcam's website and then we give 100% credit note for this antibody to buy next antibody)?

    Thanks in advance.

    Have a wonderful week.

    Read More
    Answer

    Thank you for your enquiry and your interest.
    We can certainly consider offering a testing discount code. Would you be so kind to discuss with the customer what type human samples (cells, tissue sections, fixation) will be used for testing.
    Thank you very much in advance. I look forward to hearing from you soon.

    Read More

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