Recombinant Anti-BPTF / FALZ antibody [EPR25422-35] (ab288159)
![Western blot - Anti-BPTF / FALZ antibody [EPR25422-35] (ab288159)](https://www.abcam.com/ps/products/288/ab288159/Images/ab288159-478897-anti-bptffalz-antibody-epr2542235-western-blot-adenocarcinoma2.jpg "Western blot - Anti-BPTF / FALZ antibody [EPR25422-35] (ab288159)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25422-35] to BPTF / FALZ
- Suitable for: ICC/IF, Flow Cyt (Intra), IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-BPTF / FALZ antibody [EPR25422-35]
See all BPTF / FALZ primary antibodies -
Description
Rabbit monoclonal [EPR25422-35] to BPTF / FALZ -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, Flow Cyt (Intra), IHC-P, WBmore details
Unsuitable for: ChIP or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa and HEK-293T whole cell lysates. IHC-P: Human astrocytoma, endometrial carcinoma and lung carcinoma tissues. ICC/IF: HeLa cells. Flow cyt: HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR25422-35 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab288159 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/50.
|
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| Flow Cyt (Intra) |
1/500.
|
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| IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| WB |
1/1000. Detects a band of approximately 320 kDa (predicted molecular weight: 338 kDa).
|
| Notes |
|---|
|
ICC/IF
1/50. |
|
Flow Cyt (Intra)
1/500. |
|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
WB
1/1000. Detects a band of approximately 320 kDa (predicted molecular weight: 338 kDa). |
Target
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Function
Histone-binding component of NURF (nucleosome-remodeling factor), a complex which catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin. Specifically recognizes H3 tails trimethylated on 'Lys-4' (H3K4me3), which mark transcription start sites of virtually all active genes. May also regulate transcription through direct binding to DNA or transcription factors. -
Tissue specificity
Ubiquitously expressed, with highest levels in testis. Present in kidney, liver and brain. In the brain, highest levels are found in motor cortex (at protein level). -
Sequence similarities
Belongs to the PBTF family.
Contains 1 bromo domain.
Contains 1 DDT domain.
Contains 2 PHD-type zinc fingers. -
Developmental stage
Abundantly expressed in the fetal brain. Present throughout the gray and white matter of the developing spinal cord at 18-22 gestational weeks. Expressed at low levels in adult brain and spinal cord and reexpressed in neurodegenerative diseases (at protein level). -
Domain
The second PHD-type zinc finger mediates binding to histone H3K4Me3. Has specificity for trimethyllysine; introducing a mutation in the Tyr-2876 residue can induce binding to dimethyllysine. -
Post-translational
modificationsPhosphorylation enhances DNA-binding.
Highly susceptible to proteolysis. -
Cellular localization
Cytoplasm. Nucleus. In brains of Alzheimer disease patients, present in a subset of amyloid-containing plaques. - Information by UniProt
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Database links
- Entrez Gene: 2186 Human
- Omim: 601819 Human
- SwissProt: Q12830 Human
- Unigene: 444200 Human
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Alternative names
- BPTF antibody
- BPTF_HUMAN antibody
- Bromodomain and PHD finger containing transcription factor antibody
see all
Images
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Western blot - Anti-BPTF / FALZ antibody [EPR25422-35] (ab288159)All lanes : Anti-BPTF / FALZ antibody [EPR25422-35] (ab288159) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : HeLa transfected with BPTF siRNA 1 whole cell lysate
Lane 3 : HeLa transfected with BPTF siRNA 2 whole cell lysate
Lane 4 : HeLa transfected with BPTF siRNA 3 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 338 kDa
Observed band size: 320 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The observed MW/expression profile is consistent with what has been described in the literature (PMID: 31498079).
Exposure time: 37 seconds
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![Western blot - Anti-BPTF / FALZ antibody [EPR25422-35] (ab288159)](https://www.abcam.com/ps/products/288/ab288159/Images/ab288159-478899-anti-bptffalz-antibody-epr2542235-western-blot-adenocarcinoma3.jpg)
Western blot - Anti-BPTF / FALZ antibody [EPR25422-35] (ab288159)All lanes : Anti-BPTF / FALZ antibody [EPR25422-35] (ab288159) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 338 kDa
Observed band size: 320 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Lysates should be made freshly and used in WB immediately to minimize protein degradation.
The observed MW/expression profile is consistent with what has been described in the literature (PMID: 31498079).
Exposure time: Lane1: 10 seconds; Lane2: 5.5 seconds;
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BPTF / FALZ antibody [EPR25422-35] (ab288159)](https://www.abcam.com/ps/products/288/ab288159/Images/ab288159-3-anti-bptffalz-antibody-epr2542235-immunohistochemistry-human-astrocytoma1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BPTF / FALZ antibody [EPR25422-35] (ab288159)Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labelling BPTF / FALZ with ab288159 at 1/500 (1.076 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on human astrocytoma (PMID: 30542695). The section was incubated with ab288159 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BPTF / FALZ antibody [EPR25422-35] (ab288159)](https://www.abcam.com/ps/products/288/ab288159/Images/ab288159-4-anti-bptffalz-antibody-epr2542235-immunohistochemistry-human-endometrial-carcinoma1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BPTF / FALZ antibody [EPR25422-35] (ab288159)Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labelling BPTF / FALZ with ab288159 at 1/500 (1.076 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on human endometrial carcinoma. The section was incubated with ab288159 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BPTF / FALZ antibody [EPR25422-35] (ab288159)](https://www.abcam.com/ps/products/288/ab288159/Images/ab288159-5-anti-bptffalz-antibody-epr2542235-immunohistochemistry-human-lung-carcinoma1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BPTF / FALZ antibody [EPR25422-35] (ab288159)Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelling BPTF / FALZ with ab288159 at 1/500 (1.076 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on human lung carcinoma (PMID: 26418899).The section was incubated with ab288159 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
![Immunocytochemistry/ Immunofluorescence - Anti-BPTF / FALZ antibody [EPR25422-35] (ab288159)](https://www.abcam.com/ps/products/288/ab288159/Images/ab288159-6-anti-bptffalz-antibody-epr2542235-immunocytochemistry-hela1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-BPTF / FALZ antibody [EPR25422-35] (ab288159)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling BPTF / FALZ with ab288159 at 1/50 (10.76 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
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![Flow Cytometry (Intracellular) - Anti-BPTF / FALZ antibody [EPR25422-35] (ab288159)](https://www.abcam.com/ps/products/288/ab288159/Images/ab288159-7-anti-bptffalz-antibody-epr2542235-flow-cytometry-hela1.jpg)
Flow Cytometry (Intracellular) - Anti-BPTF / FALZ antibody [EPR25422-35] (ab288159)Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling BPTF / FALZ with ab288159 at 1/500 dilution (0.1ug)(Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
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![Anti-BPTF / FALZ antibody [EPR25422-35] (ab288159)](https://www.abcam.com/ps/products/288/ab288159/Images/ab288159-8-Benefits-of-Recombinant-antibodies.jpg)
Anti-BPTF / FALZ antibody [EPR25422-35] (ab288159)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab288159 has not yet been referenced specifically in any publications.