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  1. Link

    products/primary-antibodies/brca2-antibody-ab123491.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair Homologous Recomb.
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Anti-BRCA2 antibody (ab123491)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (13)

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Western blot - Anti-BRCA2 antibody (ab123491)
  • Immunoprecipitation - Anti-BRCA2 antibody (ab123491)
  • Western blot - Anti-BRCA2 antibody (ab123491)

Key features and details

  • Rabbit polyclonal to BRCA2
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-BRCA2 antibody
    See all BRCA2 primary antibodies
  • Description

    Rabbit polyclonal to BRCA2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 450-500 of Human BRCA2 using the numbering given in entry NP_000050.1.

  • Positive control

    • Whole cell lysate from 293T, HeLa and Jurkat cells
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Homologous Recomb.
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • BRCT Domain Proteins
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human BRCA2 protein (ab112253)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab123491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/2000 - 1/10000. Predicted molecular weight: 384 kDa.
IP
Use at 2-10 µg/mg of lysate.
Notes
WB
1/2000 - 1/10000. Predicted molecular weight: 384 kDa.
IP
Use at 2-10 µg/mg of lysate.

Target

  • Function

    Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures.
  • Tissue specificity

    Highest levels of expression in breast and thymus, with slightly lower levels in lung, ovary and spleen.
  • Involvement in disease

    Defects in BRCA2 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
    Defects in BRCA2 are the cause of pancreatic cancer type 2 (PNCA2) [MIM:613347]. It is a malignant neoplasm of the pancreas. Tumors can arise from both the exocrine and endocrine portions of the pancreas, but 95% of them develop from the exocrine portion, including the ductal epithelium, acinar cells, connective tissue, and lymphatic tissue.
    Defects in BRCA2 are a cause of susceptibility to breast-ovarian cancer familial type 2 (BROVCA2) [MIM:612555]. A condition associated with familial predisposition to cancer of the breast and ovaries. Characteristic features in affected families are an early age of onset of breast cancer (often before age 50), increased chance of bilateral cancers (cancer that develop in both breasts, or both ovaries, independently), frequent occurrence of breast cancer among men, increased incidence of tumors of other specific organs, such as the prostate.
    Defects in BRCA2 are the cause of Fanconi anemia complementation group D type 1 (FANCD1) [MIM:605724]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
    Defects in BRCA2 are a cause of glioma type 3 (GLM3) [MIM:613029]. Gliomas are benign or malignant central nervous system neoplasms derived from glial cells. They comprise astrocytomas and glioblastoma multiforme that are derived from astrocytes, oligodendrogliomas derived from oligodendrocytes and ependymomas derived from ependymocytes.
  • Sequence similarities

    Contains 8 BRCA2 repeats.
  • Post-translational
    modifications

    Phosphorylated by ATM upon irradiation-induced DNA damage.
    Ubiquitinated in the absence of DNA damage; this does not lead to proteasomal degradation. In contrast, ubiquitination in response to DNA damage leads to proteasomal degradation.
  • Target information above from: UniProt accession P51587 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 675 Human
    • Omim: 600185 Human
    • SwissProt: P51587 Human
    • Unigene: 34012 Human
    • Alternative names

      • BRCA 2 antibody
      • BRCA1/BRCA2 containing complex subunit 2 antibody
      • Brca2 antibody
      • BRCA2, DNA repair associated antibody
      • BRCA2_HUMAN antibody
      • BRCC 2 antibody
      • BRCC2 antibody
      • Breast and ovarian cancer susceptibility gene early onset antibody
      • breast and ovarian cancer susceptibility protein 2 antibody
      • Breast cancer 2 early onset antibody
      • Breast Cancer 2 tumor suppressor antibody
      • Breast cancer susceptibility protein BRCA2 antibody
      • Breast cancer type 2 susceptibility protein antibody
      • BROVCA2 antibody
      • FACD antibody
      • FAD 1 antibody
      • FAD antibody
      • FAD1 antibody
      • FANCB antibody
      • FANCD 1 antibody
      • FANCD antibody
      • FANCD1 antibody
      • FANCD1 gene antibody
      • Fanconi anemia complementation group D1 antibody
      • Fanconi anemia group D1 protein antibody
      • GLM3 antibody
      • mutant BRCA2 antibody
      • OTTHUMP00000018803 antibody
      • OTTHUMP00000042401 antibody
      • PNCA2 antibody
      • XRCC11 antibody
      see all

    Images

    • Western blot - Anti-BRCA2 antibody (ab123491)
      Western blot - Anti-BRCA2 antibody (ab123491)
      All lanes : Anti-BRCA2 antibody (ab123491) at 0.1 µg/ml

      Lane 1 : 293T whole cell lysate at 50 µg
      Lane 2 : 293T whole cell lysate at 15 µg
      Lane 3 : 293T whole cell lysate at 5 µg
      Lane 5 : HeLa whole cell lysate at 50 µg
      Lane 6 : HeLa whole cell lysate at 15 µg

      Predicted band size: 384 kDa


      Exposure time: 30 seconds
    • Immunoprecipitation - Anti-BRCA2 antibody (ab123491)
      Immunoprecipitation - Anti-BRCA2 antibody (ab123491)
      Detection of Human BRCA2 by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab123491 at 6 µg/mg lysate.
      Subsequent Western blot detection used ab123491 at 1 µg/ml.
      Detection: Chemiluminescence with an exposure time of 10 seconds.
    • Western blot - Anti-BRCA2 antibody (ab123491)
      Western blot - Anti-BRCA2 antibody (ab123491)
      All lanes : Anti-BRCA2 antibody (ab123491) at 0.1 µg/ml

      Lane 1 : Whole cell lysate from HeLa at 50 µg/ml
      Lane 2 : Whole cell lysate from HeLa at 15 µg/ml
      Lane 3 : Whole cell lysate from 293T at 50 µg/ml
      Lane 4 : Whole cell lysate from Jurkat at 50 µg/ml

      Developed using the ECL technique.

      Predicted band size: 384 kDa


      Exposure time: 10 minutes

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (13)

    Publishing research using ab123491? Please let us know so that we can cite the reference in this datasheet.

    ab123491 has been referenced in 13 publications.

    • Goldsmith J  et al. Ribosome profiling reveals a functional role for autophagy in mRNA translational control. Commun Biol 3:388 (2020). PubMed: 32681145
    • Ji W  et al. Non-small cell lung cancer cells with deficiencies in homologous recombination genes are sensitive to PARP inhibitors. Biochem Biophys Res Commun 522:121-126 (2020). PubMed: 31753490
    • Shang Y  et al. MEIOK21: a new component of meiotic recombination bridges required for spermatogenesis. Nucleic Acids Res N/A:N/A (2020). PubMed: 32463460
    • Chen HD  et al. Novel mutations in BRCA2 intron 11 and overexpression of COX-2 and BIRC3 mediate cellular resistance to PARP inhibitors. Am J Cancer Res 10:2813-2831 (2020). PubMed: 33042619
    • Le HP  et al. DSS1 and ssDNA regulate oligomerization of BRCA2. Nucleic Acids Res 48:7818-7833 (2020). PubMed: 32609828
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-BRCA2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa Cells (Cervical Cancer))
    Permeabilization
    Yes - 0.5% Triton X-100 in PBS
    Specification
    HeLa Cells (Cervical Cancer)
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 04 2015

    Western blot abreview for Anti-BRCA2 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Glioblastoma and Melanoma)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    30 µg
    Specification
    Glioblastoma and Melanoma
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 22 2019

    Western blot abreview for Anti-BRCA2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    40 µg
    Gel Running Conditions
    Reduced Denaturing (6%)
    Sample
    Human Cell lysate - whole cell (HeLa cell line)
    Specification
    HeLa cell line
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 12 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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