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  1. Link

    products/primary-antibodies/brca2-antibody-ab90541.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair Homologous Recomb.
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Anti-BRCA2 antibody (ab90541)

  • Datasheet
Reviews (2)Q&A (1)References (4)

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Western blot - Anti-BRCA2 antibody (ab90541)

    Key features and details

    • Rabbit polyclonal to BRCA2
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    Overview

    • Product name

      Anti-BRCA2 antibody
      See all BRCA2 primary antibodies
    • Description

      Rabbit polyclonal to BRCA2
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Synthetic peptide (Human)

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      Constituent: PBS
    • Concentration information loading...
    • Purity

      Affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • DNA Damage & Repair
      • Homologous Recomb.
      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • DNA Damage & Repair
      • DNA Damage Response
      • BRCT Domain Proteins
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Cancer susceptibility
      • Tumor Suppressors
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Transcription Factors
      • Cancer
      • Oncoproteins/suppressors
      • Tumor suppressors
      • Other

    Associated products

    • ChIP Related Products

      • ChIP Kit (ab500)
    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human BRCA2 protein (ab112253)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab90541 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (1)
    Use at an assay dependent concentration. Predicted molecular weight: 384 kDa.
    Notes
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 384 kDa.

    Target

    • Function

      Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures.
    • Tissue specificity

      Highest levels of expression in breast and thymus, with slightly lower levels in lung, ovary and spleen.
    • Involvement in disease

      Defects in BRCA2 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
      Defects in BRCA2 are the cause of pancreatic cancer type 2 (PNCA2) [MIM:613347]. It is a malignant neoplasm of the pancreas. Tumors can arise from both the exocrine and endocrine portions of the pancreas, but 95% of them develop from the exocrine portion, including the ductal epithelium, acinar cells, connective tissue, and lymphatic tissue.
      Defects in BRCA2 are a cause of susceptibility to breast-ovarian cancer familial type 2 (BROVCA2) [MIM:612555]. A condition associated with familial predisposition to cancer of the breast and ovaries. Characteristic features in affected families are an early age of onset of breast cancer (often before age 50), increased chance of bilateral cancers (cancer that develop in both breasts, or both ovaries, independently), frequent occurrence of breast cancer among men, increased incidence of tumors of other specific organs, such as the prostate.
      Defects in BRCA2 are the cause of Fanconi anemia complementation group D type 1 (FANCD1) [MIM:605724]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
      Defects in BRCA2 are a cause of glioma type 3 (GLM3) [MIM:613029]. Gliomas are benign or malignant central nervous system neoplasms derived from glial cells. They comprise astrocytomas and glioblastoma multiforme that are derived from astrocytes, oligodendrogliomas derived from oligodendrocytes and ependymomas derived from ependymocytes.
    • Sequence similarities

      Contains 8 BRCA2 repeats.
    • Post-translational
      modifications

      Phosphorylated by ATM upon irradiation-induced DNA damage.
      Ubiquitinated in the absence of DNA damage; this does not lead to proteasomal degradation. In contrast, ubiquitination in response to DNA damage leads to proteasomal degradation.
    • Target information above from: UniProt accession P51587 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 675 Human
      • Omim: 600185 Human
      • SwissProt: P51587 Human
      • Unigene: 34012 Human
      • Alternative names

        • BRCA 2 antibody
        • BRCA1/BRCA2 containing complex subunit 2 antibody
        • Brca2 antibody
        • BRCA2, DNA repair associated antibody
        • BRCA2_HUMAN antibody
        • BRCC 2 antibody
        • BRCC2 antibody
        • Breast and ovarian cancer susceptibility gene early onset antibody
        • breast and ovarian cancer susceptibility protein 2 antibody
        • Breast cancer 2 early onset antibody
        • Breast Cancer 2 tumor suppressor antibody
        • Breast cancer susceptibility protein BRCA2 antibody
        • Breast cancer type 2 susceptibility protein antibody
        • BROVCA2 antibody
        • FACD antibody
        • FAD 1 antibody
        • FAD antibody
        • FAD1 antibody
        • FANCB antibody
        • FANCD 1 antibody
        • FANCD antibody
        • FANCD1 antibody
        • FANCD1 gene antibody
        • Fanconi anemia complementation group D1 antibody
        • Fanconi anemia group D1 protein antibody
        • GLM3 antibody
        • mutant BRCA2 antibody
        • OTTHUMP00000018803 antibody
        • OTTHUMP00000042401 antibody
        • PNCA2 antibody
        • XRCC11 antibody
        see all

      Images

      • Western blot - Anti-BRCA2 antibody (ab90541)
        Western blot - Anti-BRCA2 antibody (ab90541)
        All lanes : Anti-BRCA2 antibody (ab90541)

        Lane 1 : Capan 1
        Lane 2 : HeLa

        Predicted band size: 384 kDa
        Observed band size: 370 kDa why is the actual band size different from the predicted?

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (4)

      Publishing research using ab90541? Please let us know so that we can cite the reference in this datasheet.

      ab90541 has been referenced in 4 publications.

      • Zhong J  et al. Acetylation of hMOF Modulates H4K16ac to Regulate DNA Repair Genes in Response to Oxidative Stress. Int J Biol Sci 13:923-934 (2017). PubMed: 28808424
      • Lev A  et al. Preclinical rationale for combination of crizotinib with mitomycin C for the treatment of advanced colorectal cancer. Cancer Biol Ther 18:694-704 (2017). PubMed: 28886275
      • Zhang N  et al. FoxM1 Inhibition Sensitizes Resistant Glioblastoma Cells to Temozolomide by Downregulating the Expression of DNA-Repair Gene Rad51. Clin Cancer Res 18:5961-71 (2012). WB . PubMed: 22977194
      • Badie S  et al. BRCA2 acts as a RAD51 loader to facilitate telomere replication and capping. Nat Struct Mol Biol 17:1461-9 (2010). ChIP . PubMed: 21076401

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-3 of 3 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-BRCA2 antibody

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (pancreatic tumor)
      Specification
      pancreatic tumor
      Fixative
      Formaldehyde
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: citrate buffer pH6
      Permeabilization
      No
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted May 06 2013

      Western blot abreview for Anti-BRCA2 antibody

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (U2OS)
      Loading amount
      25 µg
      Specification
      U2OS
      Gel Running Conditions
      Reduced Denaturing
      Blocking step
      BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      DR. Andrew Cobb

      Verified customer

      Submitted Apr 03 2012

      Question

      I would like to do a BRCA2 IHC in mouse tissue and I'm looking for an antibody against mouse BRCA2. Ideally I like to have both an antibody against the N terminus and an antibody against the C terminus of murine BRCA2 protein. I've found a research article that used an Abcam BRCA2 antibody to do IHC on mouse tissue, but they don't provide the catalog number. I couldn't find more information on your website. Could you please let me know if you have such antibodies? Thank you for your attention.

      Read More

      Abcam community

      Verified customer

      Asked on Feb 26 2013

      Answer

      We do offer two anti- BRCA2 products which are guaranteed for use in mouse samples. Ab27976 is against the N-terminal region and has been referenced in publication with mouse samples. This product is tested in IHC-P and is covered by our six month Abpromise guarantee. We also offer ab90541 which is C-terminal directed and is guaranteed for reactivity with mouse. We have not yet had the opportunity to test this product in IHC on this species. However, by participating in our AbTrial program, you can now use our products in an untested application or species without financial risk.

      Read More

      Abcam Scientific Support

      Answered on Feb 26 2013

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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