Name: Anti-BRD1/BRL antibody [EPR12960]
Brand: RabMAb
SKU: ab181060
Rating: 5 (2 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD1/BRL antibody [EPR12960] (ab181060)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12960] to BRD1/BRL
Suitable for: WB, IHC-P, IP
Reacts with: Mouse, Rat, Human

Carrier Free

Product name

Anti-BRD1/BRL antibody [EPR12960]
See all BRD1/BRL primary antibodies
Description

Rabbit monoclonal [EPR12960] to BRD1/BRL
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, IPmore details
Unsuitable for: ICC/IF
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: K-562 and Raji cell lysate; IHC-P: Human testis and lung carcinoma tissue, Mouse testis tissue and Rat testis tissue. IP: K-562 whole cell lysate, Mouse testis tissue lysate
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR12960
Isotype

IgG
Research areas
- Epigenetics and Nuclear Signaling
- Bromodomains

Alternative Versions
- Anti-BRD1/BRL antibody [EPR12960] - BSA and Azide free (ab250317)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- Raji whole cell lysate (ab30124)
- K-562 whole cell lysate (ab7911)
Recombinant Protein
- Recombinant Human BRD1/BRL protein (ab187427)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab181060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB	(1)	1/1000 - 1/2000. Predicted molecular weight: 120 kDa.
IHC-P		1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP		1/30.

Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 120 kDa.
IHC-P 1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/30.

Application notes

Is unsuitable for ICC/IF.

Function

Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity.
Tissue specificity

Highly expressed in testis.
Sequence similarities

Contains 1 bromo domain.
Contains 1 PHD-type zinc finger.
Contains 1 PWWP domain.
Cellular localization

Nucleus.
Target information above from: UniProt accession O95696 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 23774 Human
- Entrez Gene: 223770 Mouse
- Entrez Gene: 315210 Rat
- Omim: 604589 Human
- SwissProt: O95696 Human
- Unigene: 127950 Human
Alternative names
- BR140-like protein antibody
- BRD1 antibody
- BRD1_HUMAN antibody
- BRL antibody
- Bromodomain and PHD finger-containing protein 2 antibody
- Bromodomain containing 1 antibody
- Bromodomain-containing protein 1 antibody
- BRPF2 antibody
- DKFZp686F0325 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD1/BRL antibody [EPR12960] (ab181060)

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling BRD1/BRL with ab181060 at 1/200 dilution (3.46 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on rat testis (PMID: 10602503). The section was incubated with ab181060 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Western blot - Anti-BRD1/BRL antibody [EPR12960] (ab181060)

All lanes : Anti-BRD1/BRL antibody [EPR12960] (ab181060) at 1/2000 dilution

Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 2 : Raji cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 120 kDa

Blocking/ Dilution buffer: 5% NFDM /TBST.
Immunoprecipitation - Anti-BRD1/BRL antibody [EPR12960] (ab181060)

ab181060 at 1/30 dilution (2μg in 0.35mg lysates) immunoprecipitating BRD1/BRL in K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate.

Lane 1 (input): K-562 whole cell lysate (10µg)

Lane 2 (+): ab181060 + K-562 whole cell lysate

Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab181060 in K-562 whole cell lysate

For western blotting, ab181060 at 1/1000 dilution (0.691 μg/mL) followed by VeriBlot for IP (HRP) (ab131366) was used for detection (1/5000).

Blocking and Diluting buffer and concentration: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD1/BRL antibody [EPR12960] (ab181060)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling BRD1/BRL with ab181060 at 1/200 dilution (3.46 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse testis (PMID: 10602503). The section was incubated with ab181060 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Immunoprecipitation - Anti-BRD1/BRL antibody [EPR12960] (ab181060)

ab181060 at 1/30 dilution (2μg in 0.35mg lysates) immunoprecipitating BRD1/BRL in mouse testis tissue lysate.

Lane 1 (input): Mouse testis tissue lysate (10µg)

Lane 2 (+): ab181060 + Mouse testis tissue lysate

Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab181060 in mouse testis tissue lysate

For western blotting, ab181060 at 1/1000 dilution (0.691 μg/mL) followed by VeriBlot for IP (HRP) (ab131366) was used for detection (1/5000).

Blocking and Diluting buffer and concentration: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD1/BRL antibody [EPR12960] (ab181060)

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling BRD1/BRL with ab181060 at 1/200 dilution (3.46 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human lung carcinoma. The section was incubated with ab181060 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD1/BRL antibody [EPR12960] (ab181060)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling BRD1/BRL with ab181060 at 1/200 dilution (3.46 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human testis (PMID: 10602503). The section was incubated with ab181060 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Anti-BRD1/BRL antibody [EPR12960] (ab181060)

Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab181060? Please let us know so that we can cite the reference in this datasheet.

ab181060 has been referenced in 2 publications.

Li Z et al. Expression Characteristics and Clinical Correlations of BRD1 in Colorectal Cancer Samples. Technol Cancer Res Treat 20:15330338211039678 (2021). PubMed: 34482774
Kulej K et al. Time-resolved Global and Chromatin Proteomics during Herpes Simplex Virus Type 1 (HSV-1) Infection. Mol Cell Proteomics 16:S92-S107 (2017). PubMed: 28179408

Customer reviews and Q&As

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Application

Western blot

Sample

Human Cell lysate - whole cell (Breast and Endometrial cell lines)

Gel Running Conditions

Reduced Denaturing (4-12% bis tris)

Loading amount

3 µg

Treatment

siCON or si-BRD1 smartpool for 24 hours

Specification

Breast and Endometrial cell lines

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Katie Lynch

Verified customer

Submitted Dec 31 2021

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Positive Controls

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (2)

Customer reviews and Q&As

Western blot abreview for Anti-BRD1/BRL antibody [EPR12960]