Recombinant Anti-BRD1/BRL antibody [EPR12960] - BSA and Azide free (ab250317)

This data was developed using ab181060, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling BRD1/BRL with ab181060 at 1/200 dilution (3.46 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on rat testis (PMID: 10602503). The section was incubated with ab181060 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

All lanes : Anti-BRD1/BRL antibody [EPR12960] (ab181060) at 1/2000 dilution

Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 2 : Raji cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 120 kDa

This data was developed using ab181060, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

ab181060 at 1/30 dilution (2μg in 0.35mg lysates) immunoprecipitating BRD1/BRL in K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate.

Lane 1 (input): K-562 whole cell lysate (10µg)

Lane 2 (+): ab181060 + K-562 whole cell lysate

Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab181060 in K-562 whole cell lysate

For western blotting, ab181060 at 1/1000 dilution (0.691 μg/mL) followed by VeriBlot for IP (HRP) (ab131366) was used for detection (1/5000).

Blocking and Diluting buffer and concentration: 5% NFDM/TBST.

This data was developed using ab181060, the same antibody clone in a different buffer formulation.

 

This data was developed using ab181060, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling BRD1/BRL with ab181060 at 1/200 dilution (3.46 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse testis (PMID: 10602503). The section was incubated with ab181060 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

ab181060 at 1/30 dilution (2μg in 0.35mg lysates) immunoprecipitating BRD1/BRL in mouse testis tissue lysate.

Lane 1 (input): Mouse testis tissue lysate (10µg)

Lane 2 (+): ab181060 + Mouse testis tissue lysate

Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab181060 in mouse testis tissue lysate

For western blotting, ab181060 at 1/1000 dilution (0.691 μg/mL) followed by VeriBlot for IP (HRP) (ab131366) was used for detection (1/5000).

Blocking and Diluting buffer and concentration: 5% NFDM/TBST.

This data was developed using ab181060, the same antibody clone in a different buffer formulation.

 

This data was developed using ab181060, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling BRD1/BRL with ab181060 at 1/200 dilution (3.46 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human lung carcinoma. The section was incubated with ab181060 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using ab181060, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling BRD1/BRL with ab181060 at 1/200 dilution (3.46 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human testis (PMID: 10602503). The section was incubated with ab181060 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).