Recombinant Anti-BRD2 antibody [BL-167-2A2] (ab243865)
![Western blot - Anti-BRD2 antibody [BL-167-2A2] (ab243865)](https://www.abcam.com/ps/products/243/ab243865/Images/ab243865-393593-anti-brd2-antibody-bl-167-2a2-western-blot-human-knockout-lysate.jpg "Western blot - Anti-BRD2 antibody [BL-167-2A2] (ab243865)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BL-167-2A2] to BRD2
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Mouse, Human
Overview
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Product name
Anti-BRD2 antibody [BL-167-2A2]
See all BRD2 primary antibodies -
Description
Rabbit monoclonal [BL-167-2A2] to BRD2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human BRD2 aa 675-725. The exact sequence is proprietary. NP_005095.1 and Gene ID 6046.
Database link: P25440 -
Positive control
- WB: Wild-type HEK293T, BRD2 knockout HEK293T, HeLa, RenCa, NIH/3T3 and C2C12 whole cell lysates. IP: HEK-293T cell lysates.
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General notes
This product is sold under License from Bethyl Laboratories, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA -
Concentration information loading... -
Purification notes
Recombinant antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BL-167-2A2 -
Isotype
IgG -
Research areas
Associated products
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab243865 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000.
|
|
| IP |
Use at an assay dependent concentration.
Use 10µl/0.5mg lysate. |
| Notes |
|---|
|
WB
1/1000. |
|
IP
Use at an assay dependent concentration. Use 10µl/0.5mg lysate. |
Target
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Function
May play a role in spermatogenesis or folliculogenesis. -
Sequence similarities
Contains 2 bromo domains.
Contains 1 ET domain. -
Domain
One bromodomain is sufficient for a partial interaction with histone H4 acetylated at 'Lys-13'. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6046 Human
- Entrez Gene: 14312 Mouse
- Omim: 601540 Human
- SwissProt: P25440 Human
- SwissProt: Q7JJ13 Mouse
- Unigene: 75243 Human
- Unigene: 3444 Mouse
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Alternative names
- BRD 2 antibody
- Brd2 antibody
- BRD2_HUMAN antibody
see all
Images
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Western blot - Anti-BRD2 antibody [BL-167-2A2] (ab243865)All lanes : Anti-BRD2 antibody [BL-167-2A2] (ab243865) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : BRD2 knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Observed band size: 110 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab243865 observed at 110 kDa. Red - loading control ab8245 observed at 36 kDa.
ab243865 Anti-BRD2 antibody [BL-167-2A2] was shown to specifically react with BRD2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267265 (knockout cell lysate ab257191) was used. Wild-type and BRD2 knockout samples were subjected to SDS-PAGE. ab243865 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunoprecipitation - Anti-BRD2 antibody [BL-167-2A2] (ab243865)](https://www.abcam.com/ps/products/243/ab243865/Images/ab243865-334255-anti-brd2-antibody-bl-167-2a2-immunoprecipitation.jpg)
Immunoprecipitation - Anti-BRD2 antibody [BL-167-2A2] (ab243865)BRD2 was immunoprecipitated from 0.5 mg HEK-293T whole cell lysate with ab243865 at 10 µl per reaction Western blot was performed on the immunoprecipitate using ab243865 at 1/1000 dilution.Lane 1: ab243865 IP in HEK-293T cell lysate.
Lane 2: Contol IgG in HEK-293T cell lysate.
Detection: Chemiluminescence with an exposure time of 3 minutes.
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![Western blot - Anti-BRD2 antibody [BL-167-2A2] (ab243865)](https://www.abcam.com/ps/products/243/ab243865/Images/ab243865-468465-anti-brd2-antibody-bl-167-2a2-western-blot-human.jpg)
Western blot - Anti-BRD2 antibody [BL-167-2A2] (ab243865)All lanes : Anti-BRD2 antibody [BL-167-2A2] (ab243865) at 1/1000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 5 : SW620 (Human colon carcinoma cell line) whole cell lysate
Lane 6 : SK-MEL-28 (Human melanoma cell line) whole cell lysate
Lane 7 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, HRP conjugated
Exposure time: 30 seconds -
![Western blot - Anti-BRD2 antibody [BL-167-2A2] (ab243865)](https://www.abcam.com/ps/products/243/ab243865/Images/ab243865-468466-anti-brd2-antibody-bl-167-2a2-western-blot-mouse.jpg)
Western blot - Anti-BRD2 antibody [BL-167-2A2] (ab243865)All lanes : Anti-BRD2 antibody [BL-167-2A2] (ab243865) at 1/1000 dilution
Lane 1 : RenCa (Mouse epithelial kidney cell line) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 3 : C2C12 (Mouse myoblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, HRP conjugated
Exposure time: 3 seconds -
![Anti-BRD2 antibody [BL-167-2A2] (ab243865)](https://www.abcam.com/ps/products/243/ab243865/Images/ab243865-4-benefits-of-recombinant-antibodies.png)
Anti-BRD2 antibody [BL-167-2A2] (ab243865)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab243865 has not yet been referenced specifically in any publications.