Recombinant Anti-BRD3 antibody [BLR069G] (ab264420)
![Western blot - Anti-BRD3 antibody [BLR069G] (ab264420)](https://www.abcam.com/ps/products/264/ab264420/Images/ab264420-366135-AntiBRD3-antibody-BLR069G-western-blot-hek293t-rko-jurkat-hela-mcf7-k562-hepg2-human.jpg "Western blot - Anti-BRD3 antibody [BLR069G] (ab264420)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR069G] to BRD3
- Suitable for: ICC, IP, WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-BRD3 antibody [BLR069G]
See all BRD3 primary antibodies -
Description
Rabbit monoclonal [BLR069G] to BRD3 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IP, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human BRD3 aa 676-726. The exact sequence is proprietary. NP_031397.1.
Database link: Q15059 -
Positive control
- IHC-P: Human lung carcinoma and colon carcinoma tissues. ICC: OVCAR-3 cells. WB: HEK-293T, RKO, Jurkat, LNCaP, HeLa, MCF7, K562 and HepG2 whole cell lysates. IP: HEK-293T whole cell lysate.
-
General notes
This product is sold under License from Bethyl Laboratories, Inc.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Borate buffered saline -
Concentration information loading... -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BLR069G -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab264420 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
1/100 - 1/500.
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. |
|
| IP |
Use at an assay dependent concentration.
Use 20 µl/mg lysate. |
|
| WB |
1/1000. Predicted molecular weight: 80 kDa.
|
|
| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
ICC
1/100 - 1/500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. |
|
IP
Use at an assay dependent concentration. Use 20 µl/mg lysate. |
|
WB
1/1000. Predicted molecular weight: 80 kDa. |
|
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Tissue specificity
Ubiquitous. -
Involvement in disease
Note=A chromosomal aberration involving BRD3 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;9)(q14;q34) with NUT which produces a BRD3-NUT fusion protein. -
Sequence similarities
Contains 2 bromo domains. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 8019 Human
- Omim: 601541 Human
- SwissProt: Q15059 Human
- Unigene: 522472 Human
- Unigene: 654869 Human
-
Alternative names
- BRD 3 antibody
- Brd3 antibody
- BRD3_HUMAN antibody
see all
Images
-
Western blot - Anti-BRD3 antibody [BLR069G] (ab264420)All lanes : Anti-BRD3 antibody [BLR069G] (ab264420) at 1/1000 dilution
Lane 1 : HEK-293T whole cell lysate
Lane 2 : RKO whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : LNcaP whole cell lysate
Lane 5 : HeLa whole cell lysate
Lane 6 : MCF7 whole cell lysate
Lane 7 : K562 whole cell lysate
Lane 8 : HepG2 whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 80 kDa
Exposure time: 10 secondsLysates prepared using NETN lysis buffer.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] (ab264420)](https://www.abcam.com/ps/products/264/ab264420/Images/ab264420-2-anti-brd3-antibody-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] (ab264420)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling BRD3 with ab264420 at 1/200 dilution. Detection: DAB. Secondary: DyLight® 594-conjugated goat anti-rabbit IgG. Counterstain: DAPI (blue).
-
![Immunocytochemistry - Anti-BRD3 antibody [BLR069G] (ab264420)](https://www.abcam.com/ps/products/264/ab264420/Images/ab264420-3-anti-brd3-antibody-immunocytochemistry.jpg)
Immunocytochemistry - Anti-BRD3 antibody [BLR069G] (ab264420)Immunocytochemical analysis of formalin-fixed, paraffin-embedded OVCAR-3 cells labeling BRD3 with ab264420 at 1/250 dilution. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] (ab264420)](https://www.abcam.com/ps/products/264/ab264420/Images/ab264420-1-anti-brd3-antibody-immunohistochemistry-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] (ab264420)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue labeling BRD3 with ab264420 at 1/250 dilution. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
-
![Immunoprecipitation - Anti-BRD3 antibody [BLR069G] (ab264420)](https://www.abcam.com/ps/products/264/ab264420/Images/ab264420-366134-AntiBRD3-antibody-BLR069G-immunoprecipitation-hek293t-human.jpg)
Immunoprecipitation - Anti-BRD3 antibody [BLR069G] (ab264420)BRD3 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab264420 at 20 µl/mg lysate. Western blot was performed from the immunoprecipitates using ab264420 at 1/1000 dilution.
Lane 1: ab264420 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.Detection: Chemiluminescence with an exposure time of 1 second.
Lysates prepared using NETN lysis buffer.
-
![Anti-BRD3 antibody [BLR069G] (ab264420)](https://www.abcam.com/ps/products/264/ab264420/Images/ab264420-6-benefits-of-recombinant-antibodies.png)
Anti-BRD3 antibody [BLR069G] (ab264420)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab264420 has been referenced in 2 publications.
- Tian T et al. BET degrader inhibits tumor progression and stem-like cell growth via Wnt/ß-catenin signaling repression in glioma cells. Cell Death Dis 11:900 (2020). PubMed: 33093476
- Zhang H et al. Targeting BET Proteins With a PROTAC Molecule Elicits Potent Anticancer Activity in HCC Cells. Front Oncol 9:1471 (2019). PubMed: 31993368