Anti-Brd4 antibody [CL1115] (ab244221)
Key features and details
- Mouse monoclonal [CL1115] to Brd4
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Brd4 antibody [CL1115]
See all Brd4 primary antibodies -
Description
Mouse monoclonal [CL1115] to Brd4 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Brd4 aa 1000-1200.
Database link: O60885 -
Epitope
Binds to an epitope located within the peptidesequence PQPAKPQQVIQHHHSasdetermined by overlapping synthetic peptides. -
Positive control
- IHC-P: Human stomach cancer, testis, cerebral cortex and kidney tissues. WB: U-251 MG cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CL1115 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab244221 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 152 kDa.
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ICC/IF | (1) |
Use a concentration of 2 - 10 µg/ml.
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Notes |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 152 kDa. |
ICC/IF
Use a concentration of 2 - 10 µg/ml. |
Target
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Function
Plays a role in a process governing chromosomal dynamics during mitosis. -
Tissue specificity
Ubiquitously expressed. -
Involvement in disease
Note=A chromosomal aberration involving BRD4 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;19)(q14;p13) with NUT which produces a BRD4-NUT fusion protein. -
Sequence similarities
Contains 2 bromo domains. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 23476 Human
- Omim: 608749 Human
- SwissProt: O60885 Human
- Unigene: 187763 Human
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Alternative names
- Brd4 antibody
- BRD4-NUT FUSION antibody
- BRD4-NUT fusion oncoprotein antibody
see all
Images
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Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labeling BRD4 using ab244221 at 10 µg/ml, showing specific staining in the nucleoplasm in green. Microtubles are visualized in red.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [CL1115] (ab244221)
Paraffin-embedded human stomach cancer tissue stained for Brd4 using ab244221 at 1/200 dilution in immunohistochemical analysis.
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All lanes : Anti-Brd4 antibody [CL1115] (ab244221) at 1 µg/ml
Lane 1 : U-251 MG (human brain glioma cell line) cell lysate transfected with control siRNA
Lane 2 : U-251 MG cell lysate transfected with target specific siRNA probe #1
Lane 3 : U-251 MG cell lysate transfected with target specific siRNA probe #2
Predicted band size: 152 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [CL1115] (ab244221)
Paraffin-embedded human testis tissue stained for Brd4 using ab244221 at 1/200 dilution in immunohistochemical analysis.
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Anti-Brd4 antibody [CL1115] (ab244221) at 1 µg/ml + U-251 MG (human brain glioma cell line) cell lysate
Predicted band size: 152 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [CL1115] (ab244221)
Paraffin-embedded human cerebral cortex tissue stained for Brd4 using ab244221 at 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [CL1115] (ab244221)
Paraffin-embedded human kidney tissue stained for Brd4 using ab244221 at 1/200 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab244221 has been referenced in 1 publication.
- Li M et al. MicroRNA-608 promotes apoptosis via BRD4 downregulation in pancreatic ductal adenocarcinoma. Oncol Lett 19:1418-1426 (2020). PubMed: 32002032