Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-Brd4 antibody [CL1115] (ab244221)

Reviews (1) Submit a question References (1)
Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody [CL1115] (ab244221)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [CL1115] (ab244221)
  • Western blot - Anti-Brd4 antibody [CL1115] (ab244221)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [CL1115] (ab244221)
  • Western blot - Anti-Brd4 antibody [CL1115] (ab244221)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [CL1115] (ab244221)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [CL1115] (ab244221)

Key features and details

  • Mouse monoclonal [CL1115] to Brd4
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Brd4 antibody [CL1115]
    See all Brd4 primary antibodies

  • Description

    Mouse monoclonal [CL1115] to Brd4

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human Brd4 aa 1000-1200.
    Database link: O60885

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Epitope

    Binds to an epitope located within the peptidesequence PQPAKPQQVIQHHHSasdetermined by overlapping synthetic peptides.

  • Positive control

    • IHC-P: Human stomach cancer, testis, cerebral cortex and kidney tissues. WB: U-251 MG cell lysate.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab244221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 152 kDa.
ICC/IF (1)
Use a concentration of 2 - 10 µg/ml.
Notes
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 152 kDa.
ICC/IF
Use a concentration of 2 - 10 µg/ml.

Target

  • Function

    Plays a role in a process governing chromosomal dynamics during mitosis.

  • Tissue specificity

    Ubiquitously expressed.

  • Involvement in disease

    Note=A chromosomal aberration involving BRD4 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;19)(q14;p13) with NUT which produces a BRD4-NUT fusion protein.

  • Sequence similarities

    Contains 2 bromo domains.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Brd4 antibody
      • BRD4-NUT FUSION antibody
      • BRD4-NUT fusion oncoprotein antibody
      • BRD4_HUMAN antibody
      • Bromodomain containing 4 antibody
      • bromodomain containing protein 4 antibody
      • Bromodomain-containing protein 4 antibody
      • CAP antibody
      • chromosome associated protein antibody
      • HUNK1 antibody
      • HUNKI antibody
      • MCAP antibody
      • Mitotic chromosome-associated protein antibody
      • Protein HUNK1 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody [CL1115] (ab244221)
      Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody [CL1115] (ab244221)

      Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labeling BRD4 using ab244221 at 10 µg/ml, showing specific staining in the nucleoplasm in green. Microtubles are visualized in red.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [CL1115] (ab244221)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [CL1115] (ab244221)

      Paraffin-embedded human stomach cancer tissue stained for Brd4 using ab244221 at 1/200 dilution in immunohistochemical analysis.

    • Western blot - Anti-Brd4 antibody [CL1115] (ab244221)
      Western blot - Anti-Brd4 antibody [CL1115] (ab244221)
      All lanes : Anti-Brd4 antibody [CL1115] (ab244221) at 1 µg/ml

      Lane 1 : U-251 MG (human brain glioma cell line) cell lysate transfected with control siRNA
      Lane 2 : U-251 MG cell lysate transfected with target specific siRNA probe #1
      Lane 3 : U-251 MG cell lysate transfected with target specific siRNA probe #2

      Predicted band size: 152 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [CL1115] (ab244221)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [CL1115] (ab244221)

      Paraffin-embedded human testis tissue stained for Brd4 using ab244221 at 1/200 dilution in immunohistochemical analysis.

    • Western blot - Anti-Brd4 antibody [CL1115] (ab244221)
      Western blot - Anti-Brd4 antibody [CL1115] (ab244221)
      Anti-Brd4 antibody [CL1115] (ab244221) at 1 µg/ml + U-251 MG (human brain glioma cell line) cell lysate

      Predicted band size: 152 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [CL1115] (ab244221)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [CL1115] (ab244221)

      Paraffin-embedded human cerebral cortex tissue stained for Brd4 using ab244221 at 1/200 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [CL1115] (ab244221)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [CL1115] (ab244221)

      Paraffin-embedded human kidney tissue stained for Brd4 using ab244221 at 1/200 dilution in immunohistochemical analysis.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab244221? Please let us know so that we can cite the reference in this datasheet.

    ab244221 has been referenced in 1 publication.

    • Li M  et al. MicroRNA-608 promotes apoptosis via BRD4 downregulation in pancreatic ductal adenocarcinoma. Oncol Lett 19:1418-1426 (2020). PubMed: 32002032

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

    Filter by Species

    Filter by Ratings

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Brd4 antibody [CL1115]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Bone Osteosarcoma Epithelial Cells)
    Permeabilization
    Yes - 0.2% triton
    Specification
    Bone Osteosarcoma Epithelial Cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 29 2021

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com