Recombinant Anti-BRD7 antibody [2D3] (ab252820)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [2D3] (ab252820)](https://www.abcam.com/ps/products/252/ab252820/Images/ab252820-383315-AntiBRD7-antibody-2D3-ihc-rat-testis.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [2D3] (ab252820)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [2D3] to BRD7
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-BRD7 antibody [2D3]
See all BRD7 primary antibodies -
Description
Rat monoclonal [2D3] to BRD7 -
Host species
Rat -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is considered to be commercially sensitive.
-
Positive control
- WB: HeLa, C6, RAW264.7, PC-12, MCF7 and PC-3 whole cell lysate. Mouse brain tissue lysate. IHC-P: Human colon carcinoma tissue. Mouse and rat testis tissue.
-
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
2D3 -
Isotype
IgG2a -
Research areas
Associated products
-
Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab252820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.567 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 74 kDa).
|
|
| IHC-P |
Use a concentration of 5.67 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
Use a concentration of 0.567 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 74 kDa). |
|
IHC-P
Use a concentration of 5.67 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Acts both as coactivator and as corepressor. May play a role in chromatin remodeling. Activator of the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity. Induces dephosphorylation of GSK3B at 'Tyr-216'. Down-regulates TRIM24-mediated activation of transcriptional activation by AR (By similarity). Transcriptional corepressor that down-regulates the expression of target genes. Binds to target promoters, leading to increased histone H3 acetylation at 'Lys-9' (H3K9ac). Binds to the ESR1 promoter. Recruits BRCA1 and POU2F1 to the ESR1 promoter. Coactivator for TP53-mediated activation of transcription of a set of target genes. Required for TP53-mediated cell-cycle arrest in response to oncogene activation. Promotes acetylation of TP53 at 'Lys-382', and thereby promotes efficient recruitment of TP53 to target promoters. Inhibits cell cycle progression from G1 to S phase. -
Sequence similarities
Contains 1 bromo domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 29117 Human
- Entrez Gene: 26992 Mouse
- Entrez Gene: 361374 Rat
- SwissProt: Q9NPI1 Human
- SwissProt: O88665 Mouse
- Unigene: 437894 Human
- Unigene: 5400 Mouse
-
Alternative names
- 75 kDa bromodomain protein antibody
- BP75 antibody
- BRD 7 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [2D3] (ab252820)
Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling BRD7 with ab252820 at 5.67µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on rat testis tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using citrate buffer (pH 6.0, epitope retrieval solution 1). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [2D3] (ab252820)](https://www.abcam.com/ps/products/252/ab252820/Images/ab252820-383314-AntiBRD7-antibody-2D3-ihc-mouse-testis.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [2D3] (ab252820)Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling BRD7 with ab252820 at 5.67µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on mouse testis tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using citrate buffer (pH 6.0, epitope retrieval solution 1). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [2D3] (ab252820)](https://www.abcam.com/ps/products/252/ab252820/Images/ab252820-383313-AntiBRD7-antibody-2D3-ihc-human-colon-carcinoma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [2D3] (ab252820)Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling BRD7 with ab252820 at 5.67µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on human colon carcinoma tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using citrate buffer (pH 6.0, epitope retrieval solution 1). -
![Western blot - Anti-BRD7 antibody [2D3] (ab252820)](https://www.abcam.com/ps/products/252/ab252820/Images/ab252820-383312-AntiBRD7-antibody-2D3-wb-humanrat-and-mouse-cell-lysates.jpg)
Western blot - Anti-BRD7 antibody [2D3] (ab252820)All lanes : Anti-BRD7 antibody [2D3] (ab252820) at 0.567 µg/ml
Lane 1 : C6 (rat glial tumor glial cell), whole cell lysate
Lane 2 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution
Predicted band size: 74 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 15 secs.
-
![Western blot - Anti-BRD7 antibody [2D3] (ab252820)](https://www.abcam.com/ps/products/252/ab252820/Images/ab252820-383311-AntiBRD7-antibody-2D3-wb-human-and-mouse-cell-lysates-and-tissue.jpg)
Western blot - Anti-BRD7 antibody [2D3] (ab252820)All lanes : Anti-BRD7 antibody [2D3] (ab252820) at 0.567 µg/ml
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : PC-3 (human prostate adenocarcinoma epithelial cell), whole cell lysate
Lane 3 : Mouse brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution
Predicted band size: 74 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1-2: 15 secs; Lane 3: 3 mins.
-
![Anti-BRD7 antibody [2D3] (ab252820)](https://www.abcam.com/ps/products/252/ab252820/Images/ab252820-6-benefits-of-recombinant-antibodies.png)
Anti-BRD7 antibody [2D3] (ab252820)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab252820 has not yet been referenced specifically in any publications.