Recombinant Anti-BRD7 antibody [EPR26709-4] (ab307140)
![Western blot - Anti-BRD7 antibody [EPR26709-4] (ab307140)](https://www.abcam.com/ps/products/307/ab307140/Images/ab307140-498344-anti-brd7-antibody-epr26709-4-western-blot-sw620-human-colorectal-adenocarcinoma-epithelial-cell-whole-cell-lysate-50-ug.jpg "Western blot - Anti-BRD7 antibody [EPR26709-4] (ab307140)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR26709-4] to BRD7
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-BRD7 antibody [EPR26709-4]
See all BRD7 primary antibodies -
Description
Rabbit monoclonal [EPR26709-4] to BRD7 -
Host species
Rabbit -
Specificity
IHC-P: Unsuitable for human samples.
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Tested applications
Suitable for: WB, IHC-P, IPmore details
Unsuitable for: ChIP,Flow Cyt (Intra),ICC/IF or IHC-Fr -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, Jurkat, 293T, NIH/3T3, RAW264.7, 2.4G2, PC-12 and SW620 whole cell lysate. IHC-P: Mouse testis and cerebrum tissue. Rat cerebrum tissue. IP: HeLa and PC-12 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR26709-4 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab307140 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 74 kDa.
|
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| IHC-P |
1/100.
|
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| IP |
1/30.
|
| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 74 kDa. |
|
IHC-P
1/100. |
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IP
1/30. |
Target
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Function
Acts both as coactivator and as corepressor. May play a role in chromatin remodeling. Activator of the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity. Induces dephosphorylation of GSK3B at 'Tyr-216'. Down-regulates TRIM24-mediated activation of transcriptional activation by AR (By similarity). Transcriptional corepressor that down-regulates the expression of target genes. Binds to target promoters, leading to increased histone H3 acetylation at 'Lys-9' (H3K9ac). Binds to the ESR1 promoter. Recruits BRCA1 and POU2F1 to the ESR1 promoter. Coactivator for TP53-mediated activation of transcription of a set of target genes. Required for TP53-mediated cell-cycle arrest in response to oncogene activation. Promotes acetylation of TP53 at 'Lys-382', and thereby promotes efficient recruitment of TP53 to target promoters. Inhibits cell cycle progression from G1 to S phase. -
Sequence similarities
Contains 1 bromo domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 29117 Human
- Entrez Gene: 26992 Mouse
- Entrez Gene: 361374 Rat
- SwissProt: Q9NPI1 Human
- SwissProt: O88665 Mouse
- Unigene: 437894 Human
- Unigene: 5400 Mouse
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Alternative names
- 75 kDa bromodomain protein antibody
- BP75 antibody
- BRD 7 antibody
see all
Images
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Western blot - Anti-BRD7 antibody [EPR26709-4] (ab307140)All lanes : Anti-BRD7 antibody [EPR26709-4] (ab307140) at 1/1000 dilution
Lane 1 : SW620 (human colorectal adenocarcinoma epithelial cell), whole cell lysate 50 µg
Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate 50 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: HepG2 (PMID: 19909775).
Exposure time: 81 seconds.
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![Western blot - Anti-BRD7 antibody [EPR26709-4] (ab307140)](https://www.abcam.com/ps/products/307/ab307140/Images/ab307140-498350-anti-brd7-antibody-epr26709-4-western-blot-hela-human-cervix-adenocarcinoma-epithelial-cell-whole-cell-lysate-20-ug.jpg)
Western blot - Anti-BRD7 antibody [EPR26709-4] (ab307140)All lanes : Anti-BRD7 antibody [EPR26709-4] (ab307140) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 20 µg
Lane 2 : 293T (human embryonic kidney epithelial cell), whole cell lysate 20 µg
Lane 3 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate 20 µg
Lane 4 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
Exposure time: 180 seconds.
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![Western blot - Anti-BRD7 antibody [EPR26709-4] (ab307140)](https://www.abcam.com/ps/products/307/ab307140/Images/ab307140-498353-anti-brd7-antibody-epr26709-4-western-blot-jurkat-human-t-cell-leukemia-t-lymphocyte-whole-cell-lysate-20ug.jpg)
Western blot - Anti-BRD7 antibody [EPR26709-4] (ab307140)All lanes : Anti-BRD7 antibody [EPR26709-4] (ab307140) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate 20µg
Lane 2 : Raw264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate 20µg
Lane 3 : 2.4G2 (rat B cell lymphoma B lymphocyte), whole cell lysate 20µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
Exposure time: 180 seconds.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [EPR26709-4] (ab307140)](https://www.abcam.com/ps/products/307/ab307140/Images/ab307140-4-anti-brd7-antibody-epr26709-4-immunohistochemistry-mouse-testis.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [EPR26709-4] (ab307140)Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling BRD7 with ab307140 at 1/100 dilution (4.94 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse testis.
The section was incubated with ab307140 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [EPR26709-4] (ab307140)](https://www.abcam.com/ps/products/307/ab307140/Images/ab307140-5-anti-brd7-antibody-epr26709-4-immunohistochemistry-mouse-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [EPR26709-4] (ab307140)Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling BRD7 with ab307140 at 1/100 dilution (4.94 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse cerebrum.
The section was incubated with ab307140 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [EPR26709-4] (ab307140)](https://www.abcam.com/ps/products/307/ab307140/Images/ab307140-6-anti-brd7-antibody-epr26709-4-immunohistochemistry-rat-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD7 antibody [EPR26709-4] (ab307140)Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling BRD7 with ab307140 at 1/100 dilution (4.94 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat cerebrum.
The section was incubated with ab307140 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
-
![Immunoprecipitation - Anti-BRD7 antibody [EPR26709-4] (ab307140)](https://www.abcam.com/ps/products/307/ab307140/Images/ab307140-2-anti-brd7-antibody-epr26709-4-immunoprecipitation-hela.jpg)
Immunoprecipitation - Anti-BRD7 antibody [EPR26709-4] (ab307140)BRD7 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 10 µg with ab307140 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307140 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: HeLa whole cell lysate 10 µg
Lane 2: ab307140 IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab307140 in Hela whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 180 seconds.
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![Immunoprecipitation - Anti-BRD7 antibody [EPR26709-4] (ab307140)](https://www.abcam.com/ps/products/307/ab307140/Images/ab307140-3-anti-brd7-antibody-epr26709-4-immunoprecipitation-pc-12.jpg)
Immunoprecipitation - Anti-BRD7 antibody [EPR26709-4] (ab307140)BRD7 was immunoprecipitated from 0.35 mg PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate 10 µg with ab307140 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307140 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: PC-12 whole cell lysate 10 µg
Lane 2: ab307140 IP in PC-12 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab307140 in PC-12 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 180 seconds.
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![Anti-BRD7 antibody [EPR26709-4] (ab307140)](https://www.abcam.com/ps/products/307/ab307140/Images/ab307140-1-benefitsofrecombinantantibodies.jpg)
Anti-BRD7 antibody [EPR26709-4] (ab307140)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab307140 has not yet been referenced specifically in any publications.