Anti-BrdU antibody [IIB5] (ab8955)
![Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (ab8955)](https://www.abcam.com/ps/products/8/ab8955/Images/ab8955-287553-anti-brdu-antibody-iib5-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (ab8955)")
Key features and details
- Mouse monoclonal [IIB5] to BrdU
- Suitable for: ICC/IF, Flow Cyt, IHC-Fr, IHC-P
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-BrdU antibody [IIB5]
See all BrdU primary antibodies -
Description
Mouse monoclonal [IIB5] to BrdU -
Host species
Mouse -
Specificity
This antibody also reacts with bromodeoxyuridine when incorporated into nuclear DNA. It cross reacts with iododeoxyuridine (IdU). -
Tested applications
Suitable for: ICC/IF, Flow Cyt, IHC-Fr, IHC-Pmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to BrdU conjugated to bovine serum albumin.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
IIB5 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8955 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
Using immunocytochemistry, a combination of this antibody and Mouse monoclonal to Mitotic Cells (ab8956) can distinguish and quantitate the four major fractions of the cell cycle. |
|
| Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| IHC-Fr |
1/100 - 1/200.
ABC method. |
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| IHC-P | (1) |
1/100 - 1/200.
ABC method. |
| Notes |
|---|
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ICC/IF
Use at an assay dependent concentration. Using immunocytochemistry, a combination of this antibody and Mouse monoclonal to Mitotic Cells (ab8956) can distinguish and quantitate the four major fractions of the cell cycle. |
|
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
IHC-Fr
1/100 - 1/200. ABC method. |
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IHC-P
1/100 - 1/200. ABC method. |
Target
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Relevance
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis. -
Cellular localization
Nuclear -
Alternative names
- Bromodeoxyuridine antibody
- BUdr antibody
Images
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Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (ab8955)
Indirect immunofluorescence staining of BrdU-labeled MR65 lung cancer cells with ab8955.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (ab8955)](https://www.abcam.com/ps/products/8/ab8955/Images/ab8955-301028-anti-brdu-antibody-iib5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (ab8955)BrdU staining in sponge Halisarca caerulea using ab8955. Metacrylate embedded tissue sections were incubated with 1:200 diluted primary antibody. An avidin-biotin complex kit using biotinylated rabbit anti-mouse antibody and then incubation in avidin-biotin-peroxidase complex was used for detection.
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![Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (ab8955)](https://www.abcam.com/ps/products/8/ab8955/Images/ab8955-391620-anti-brdu-antibody-iib5-immunocytochemistry-mr65.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (ab8955)Indirect immunofluorescence staining of BrdU-labeled MR65 lung cancer cells using ab8955 (1:1000).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (ab8955)](https://www.abcam.com/ps/products/8/ab8955/Images/ab8955-301027-anti-brdu-antibody-iib5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (ab8955)BrdU staining in paraffin embedded rat intestine using ab8955. Primary antibody diluted 1:200. Peroxidase-conjugated goat anti mouse secondary antibody was used for detection.
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![Flow Cytometry - Anti-BrdU antibody [IIB5] (ab8955)](https://www.abcam.com/ps/products/8/ab8955/Images/ab8955-301026-anti-brdu-antibody-iib5-flow-cytometry.jpg)
Flow Cytometry - Anti-BrdU antibody [IIB5] (ab8955)Flow cytometric BrdU-DNA cell cycle analysis in a lung cancer cell line. Dilution 1:100 of primary antibody ab8955. FITC-conjugated goat anti mouse secondary antibody was used for detection.
Protocols
Datasheets and documents
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Datasheet download
References (27)
ab8955 has been referenced in 27 publications.
- Rozen-Gagnon K et al. Argonaute-CLIP delineates versatile, functional RNAi networks in Aedes aegypti, a major vector of human viruses. Cell Host Microbe 29:834-848.e13 (2021). PubMed: 33794184
- O'Connor S et al. AGO HITS-CLIP reveals distinct miRNA regulation of white and brown adipose tissue identity. Genes Dev 35:771-781 (2021). PubMed: 33832988
- Viais R et al. Augmin deficiency in neural stem cells causes p53-dependent apoptosis and aborts brain development. Elife 10:N/A (2021). PubMed: 34427181
- Hale CR et al. FMRP regulates mRNAs encoding distinct functions in the cell body and dendrites of CA1 pyramidal neurons. Elife 10:N/A (2021). PubMed: 34939924
- Naveed A et al. NEAT1 polyA-modulating antisense oligonucleotides reveal opposing functions for both long non-coding RNA isoforms in neuroblastoma. Cell Mol Life Sci 78:2213-2230 (2021). PubMed: 32914209
