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    products/primary-antibodies/btk-antibody-10d11-ab254141.pdf

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Signal Transduction Protein Phosphorylation Tyrosine Kinases Other
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Recombinant

Recombinant Anti-BTK antibody [10D11] (ab254141)

  • Datasheet
  • SDS
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Western blot - Anti-BTK antibody [10D11] (ab254141)
  • Anti-BTK antibody [10D11] (ab254141)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [10D11] to BTK
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-BTK antibody [10D11]
    See all BTK primary antibodies
  • Description

    Mouse monoclonal [10D11] to BTK
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Raji, Ramos, K562 and NAMALWA whole cell lysates.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    10D11
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other
    • Immunology
    • Innate Immunity
    • TLR Signaling

Associated products

  • Alternative Versions

    • Anti-BTK antibody [10D11] - BSA and Azide free (ab255892)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab254141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).
Notes
WB
1/1000. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).
Application notes
Is unsuitable for Flow Cyt,IHC-P or IP.

Target

  • Function

    Plays a crucial role in B-cell ontogeny. Transiently phosphorylates GTF2I on tyrosine residues in response to B-cell receptor cross-linking. Required for the formation of functional ARID3A DNA-binding complexes.
  • Involvement in disease

    Defects in BTK are the cause of X-linked agammaglobulinemia (XLA) [MIM:300755]; also known as X-linked agammaglobulinemia type 1 (AGMX1) or immunodeficiency type 1 (IMD1). XLA is a humoral immunodeficiency disease which results in developmental defects in the maturation pathway of B-cells. Affected boys have normal levels of pre-B-cells in their bone marrow but virtually no circulating mature B-lymphocytes. This results in a lack of immunoglobulins of all classes and leads to recurrent bacterial infections like otitis, conjunctivitis, dermatitis, sinusitis in the first few years of life, or even some patients present overwhelming sepsis or meningitis, resulting in death in a few hours. Treatment in most cases is by infusion of intravenous immunoglobulin.
    Defects in BTK may be the cause of X-linked hypogammaglobulinemia and isolated growth hormone deficiency (XLA-IGHD) [MIM:307200]; also known as agammaglobulinemia and isolated growth hormone deficiency or Fleisher syndrome or isolated growth hormone deficiency type 3 (IGHD3). In rare cases XLA is inherited together with isolated growth hormone deficiency (IGHD).
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
    Contains 1 Btk-type zinc finger.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Post-translational
    modifications

    Autophosphorylated on Tyr-223 and Tyr-551. Phosphorylation of Tyr-223 may create a docking site for a SH2 containing protein.
  • Cellular localization

    Cytoplasm. Membrane. Nucleus.
  • Target information above from: UniProt accession Q06187 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 695 Human
    • Omim: 300300 Human
    • SwissProt: Q06187 Human
    • Unigene: 159494 Human
    • Alternative names

      • Agammaglobulinaemia tyrosine kinase antibody
      • AGMX 1 antibody
      • AGMX1 antibody
      • AT antibody
      • ATK antibody
      • B cell progenitor kinase antibody
      • B-cell progenitor kinase antibody
      • BPK antibody
      • Bruton agammaglobulinemia tyrosine kinase antibody
      • Bruton tyrosine kinase antibody
      • Bruton’s Tyrosine Kinase antibody
      • Btk antibody
      • BTK_HUMAN antibody
      • dominant-negative kinase-deficient Brutons tyrosine kinase antibody
      • IMD 1 antibody
      • IMD1 antibody
      • MGC126261 antibody
      • MGC126262 antibody
      • OTTHUMP00000063593 antibody
      • PSCTK 1 antibody
      • PSCTK1 antibody
      • truncated Bruton agammaglobulinemia tyrosine kinase antibody
      • Tyrosine protein kinase BTK antibody
      • Tyrosine-protein kinase BTK antibody
      • tyrosine-protein kinase BTK isoform (lacking exon 14 antibody
      • XLA antibody
      see all

    Images

    • Western blot - Anti-BTK antibody [10D11] (ab254141)
      Western blot - Anti-BTK antibody [10D11] (ab254141)
      All lanes : Anti-BTK antibody [10D11] (ab254141) at 1/1000 dilution

      Lane 1 : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate
      Lane 2 : Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate
      Lane 3 : NAMALWA (human Burkitt's lymphoma B lymphocyte), whole cell lysate
      Lane 4 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
      Lane 5 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
      Lane 6 : MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/100000 dilution

      Predicted band size: 76 kDa
      Observed band size: 76 kDa


      Exposure time: 114 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

      This blot was developed using a higher sensitivity ECL substrate.

      Negative control: Jurkat, MOLT-4 (PMID 24759210).

    • Anti-BTK antibody [10D11] (ab254141)
      Anti-BTK antibody [10D11] (ab254141)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab254141? Please let us know so that we can cite the reference in this datasheet.

    ab254141 has been referenced in 1 publication.

    • Bykowsky MJ  et al. Discordant phenotype in siblings with X-linked agammaglobulinemia. Am J Hum Genet 58:477-83 (1996). PubMed: 8644706

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